BBa_I742140
type: ComponentDefinition
persistent identity: BBa I742140
display id: BBa_I742140
version: 1
was generated by: iGEM to SBOL conversion
description: Vanillin Biosynthesis genes
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Both Sam genes (Sam5 and Sam8) were cloned from the bacterium from Saccharothrix espanaensis COMT cDNA sequence is from Medicago sativa (the alfalfa plant), but the actual construct was synthesised by gene art. Ech and Fcs genes both come from the bacterium Pseudomonas fluorescens. References for these genes: Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix esanaensis, Berner M, et al, Journal of Bacteriology 188(7): 2666, (2006) Gowri G, et al, Molecular cloning and expression of alfalfa S-adenosyl-L-methionine: caffeic acid 3-0-methyltransferase, a key enzyme of lignin biosynthesis, Plant Physiology 97(1):7 (1991) Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin, Guo D, et al, Plant Cell. January; 13(1): 73 (2001) Cloning and Characterization of the Ferulic Acid Catabolic Genes of Sphingomonas paucimobilis SYK-6, E Masai, et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(9): 4416???4424 (2002) Vanillin production using metabolically engineered Escherichia coli under non-growing conditions, P Barghini, et al, Microbial Cell Factories, 6:13 (2007) Characterization of a bacterial tyrosine ammonia lyase, a biosynthetic enzyme for the photoactive yellow Protein, JA Kyndt et al, FEBS Letters 512: 240-244 (2002) Vanilla flavour: production by conventional and biotechnological routes, S Ramachandra Rao & GA Ravishankar, Journal of the Science of Food and Agriculture, 80:289-304 (2000) Two O-Methyltransferases Isolated from Flower Petals of Rosa chinensis var. spontanea Involved in Scent Biosynthesis W Shuiqin, et al, Journal of Bioscience and Bioengineering, 96(2): 119-128 (2003) Applied biocatalysis for the synthesis of natural flavour compounds ??? current industrial processes and future prospects, J Schrader, Biotechnology Letters, 26: 463???472 (2004)
owned by: myers
owned by: james
mutable description: Construct contains all five genes required to synthesise vanillin (the major component of vanilla scent) from tyrosine. The five genes in this pathway are, Sam5, Sam8, COMT, ech and fcs. Details of the vanillin biosynthesis pathway may be found on the [http://parts.mit.edu/igem07/index.php/Edinburgh/Yoghurt/Design#Vanilla_Flavour_Production Edinburgh Yoghurt Wiki]
discontinued: false
dominant: false
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: COMT, ech and fcs were all synthesised by geneart. The genomic COMT clone contained introns, so we decided to have the cDNA sequence synthesised by geneart Ech and Fcs genes both contain a very high GC content, which presented many difficulties during cloning. Therefore both genes were synthesised by geneart so they both had optimum codon usage for E. coli
star: false
creator: sarah hollingshead
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I742106
component: BBa_I742105
component: BBa_I742112
component: BBa_J15001
component: BBa_I742107
component: BBa_I742114
sequence annotation: BBa_I742105
sequence annotation: BBa_I742106
sequence annotation: BBa_J15001
sequence annotation: BBa_I742107
sequence annotation: BBa_I742114
sequence annotation: BBa_I742112
sequence: https://synbiohub.org/public/igem/BBa_I742140_sequence/1

BBa_I742106
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BBa_I742105
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BBa_I742112
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BBa_J15001
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BBa_I742107
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display id: component1952440
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definition: COMT
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BBa_J15001
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BBa_I742114
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BBa_J15001
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BBa_I742105
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BBa_J15001
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BBa_I742112
type: SequenceAnnotation
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display id: annotation1952452
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BBa_I742105
type: ComponentDefinition
persistent identity: BBa I742105
display id: BBa_I742105
version: 1
was generated by: iGEM to SBOL conversion
description: Sam5 gene with ribosome binding site
created: 2007-10-05T11:00:00Z
modified: 2015-08-31T04:08:02Z
mutable provenance: Saccharothrix espeanensis genome (bactierum)
owned by: james
owned by: myers
mutable description: Sam5 encodes a 4-coumarate 3-hydroxylase. The enzymes substrate is p-coumaric acid, which is converted to caffeic acid. This gene is part of the vanillin biosynthesis pathway.
discontinued: false
dominant: true
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Added a ribosome binding site to the sequence
star: false
creator: sarah hollingshead
type: DnaRegion
role: partType/Composite
role: SO:0000804
sequence annotation: rbs
sequence annotation: sam5 coding region
sequence: https://synbiohub.org/public/igem/BBa_I742105_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1949955
display id: annotation1949955
version: 1
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role: feature/rbs
role: SO:0000139

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sam5 coding region
type: SequenceAnnotation
persistent identity: annotation1949956
display id: annotation1949956
version: 1
top level: BBa_I742105
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role: feature/cds
role: SO:0000316

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persistent identity: range1949956
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BBa_I742106
type: ComponentDefinition
persistent identity: BBa I742106
display id: BBa_I742106
version: 1
was generated by: iGEM to SBOL conversion
description: Sam8 gene with ribosome binding site
created: 2007-10-05T11:00:00Z
modified: 2015-08-31T04:08:02Z
mutable provenance: Saccharothirx espeanensis genome
owned by: james
owned by: myers
mutable description: Sam8 encodes a tyrosine ammonia lyase. Enzymes substrate is tyrosine, which is deamminated to p-coumaric acid
discontinued: false
dominant: true
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: sarah hollingshead
type: DnaRegion
role: SO:0000804
role: partType/Composite
sequence annotation: native rbs
sequence annotation: Sam8 coding region
sequence: https://synbiohub.org/public/igem/BBa_I742106_sequence/1

native rbs
type: SequenceAnnotation
persistent identity: annotation1949973
display id: annotation1949973
version: 1
top level: BBa_I742106
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role: SO:0000139
role: feature/rbs

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persistent identity: range1949973
display id: range1949973
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Sam8 coding region
type: SequenceAnnotation
persistent identity: annotation1949974
display id: annotation1949974
version: 1
top level: BBa_I742106
location: https://synbiohub.org/public/igem/BBa_I742106/annotation1949974/range1949974/1
role: SO:0000316
role: feature/cds

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persistent identity: range1949974
display id: range1949974
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start: 15
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orientation: inline

COMT
type: ComponentDefinition
persistent identity: BBa I742107
display id: BBa_I742107
version: 1
was generated by: iGEM to SBOL conversion
description: COMT
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
mutable provenance: Alfalfa cDNA Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix esanaensis, Berner M, et al, Journal of Bacteriology 188(7): 2666, (2006) Gowri G, et al, Molecular cloning and expression of alfalfa S-adenosyl-L-methionine: caffeic acid 3-0-methyltransferase, a key enzyme of lignin biosynthesis, Plant Physiology 97(1):7 (1991) Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin, Guo D, et al, Plant Cell. January; 13(1): 73 (2001)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: COMT coding sequence Encodes caffeic acid-O-methyl transferase (methylates -OH on C4 of the aromatic ring) from Medicago sativa (alfalfa), synthesised by Geneart
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Used cDNA as coding gene in Alfalfa genome contains introns.
star: true
creator: sarah hollingshead
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: COMT coding region
sequence: https://synbiohub.org/public/igem/BBa_I742107_sequence/1

COMT coding region
type: SequenceAnnotation
persistent identity: annotation1949817
display id: annotation1949817
version: 1
top level: COMT
location: https://synbiohub.org/public/igem/BBa_I742107/annotation1949817/range1949817/1
role: SO:0000316
role: feature/cds

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type: Range
persistent identity: range1949817
display id: range1949817
version: 1
top level: COMT
direction: direction/forward
start: 1
end: 1101
orientation: inline

BBa_I742112
type: ComponentDefinition
persistent identity: BBa I742112
display id: BBa_I742112
version: 1
was generated by: iGEM to SBOL conversion
description: enoyl CoA hydratase (ech) gene
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
mutable provenance: Pseudomonas fluorescens NCIMB 9046 Cloning and Characterization of the Ferulic Acid Catabolic Genes of Sphingomonas paucimobilis SYK-6, E Masai, et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(9): 4416???4424 (2002)
owned by: james
owned by: myers
mutable description: enoyl CoA hydratase (ech) was isolated from Pseudomonas fluorescens genome. Ech converts feruloyl CoA to vanillin (fig. 3) and catalyses the final stage of the vanillin biosynthesis pathway. Involved in the synthesis of 'natural' vanilla flavouring
discontinued: false
dominant: false
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Gene was synthesised by geneart as we experienced problems isolating native gene, due to the high GC nature of the Pseudomonas genome. The ech sequence has been optimised for expression in E. coli
star: false
creator: sarah hollingshead
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start
sequence annotation: ech coding region
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_I742112_sequence/1

start
type: SequenceAnnotation
persistent identity: annotation1949783
display id: annotation1949783
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role: SO:0000318
role: feature/start

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ech coding region
type: SequenceAnnotation
persistent identity: annotation1949782
display id: annotation1949782
version: 1
top level: BBa_I742112
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role: feature/cds
role: SO:0000316

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stop
type: SequenceAnnotation
persistent identity: annotation1949784
display id: annotation1949784
version: 1
top level: BBa_I742112
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role: feature/stop
role: SO:0000319

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display id: range1949784
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BBa_I742114
type: ComponentDefinition
persistent identity: BBa I742114
display id: BBa_I742114
version: 1
was generated by: iGEM to SBOL conversion
description: Feruloyl-CoA synthase (fcs)
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
mutable provenance: Pseudomonas fluorescens NCIMB 9046 Cloning and Characterization of the Ferulic Acid Catabolic Genes of Sphingomonas paucimobilis SYK-6, E Masai, et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(9): 4416???4424 (2002)
owned by: james
owned by: myers
mutable description: gene is from Pseudomonas fluorescens encodes feruoyl CoA synthase (ligates acetyl-CoA onto ferulic acid) produces feruloyl CoA from ferrulic acid catalyses the penultimate of the vanillin biosynthesis pathway
discontinued: false
dominant: false
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Gene was synthesised by geneart, as P. fluorescens has a high GC genome. Coding region was optimised for gene expression in E. coli
star: false
creator: sarah hollingshead
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: fcs coding region
sequence: https://synbiohub.org/public/igem/BBa_I742114_sequence/1

fcs coding region
type: SequenceAnnotation
persistent identity: annotation1949792
display id: annotation1949792
version: 1
top level: BBa_I742114
location: https://synbiohub.org/public/igem/BBa_I742114/annotation1949792/range1949792/1
role: feature/cds
role: SO:0000316

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type: Range
persistent identity: range1949792
display id: range1949792
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start: 1
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orientation: inline

BBa_J15001
type: ComponentDefinition
persistent identity: BBa J15001
display id: BBa_J15001
version: 1
was generated by: iGEM to SBOL conversion
description: strong synthetic E. coli ribosome binding site with SacI site.
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
mutable provenance: Synthetic.
owned by: james
owned by: myers
mutable description: This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
star: false
creator: Chris French
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: SacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J15001_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1938045
display id: annotation1938045
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
role: SO:0000110
role: feature/misc

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type: Range
persistent identity: range1938045
display id: range1938045
version: 1
top level: BBa_J15001
direction: direction/forward
start: 1
end: 3
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1938046
display id: annotation1938046
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
role: SO:0000139
role: feature/rbs

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type: Range
persistent identity: range1938046
display id: range1938046
version: 1
top level: BBa_J15001
direction: direction/forward
start: 4
end: 10
orientation: inline

https://synbiohub.org/public/igem/BBa_I742140_sequence/1
type: Sequence
persistent identity: BBa I742140 sequence
display id: BBa_I742140_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acggagaagcagcgaaatgaccatcacgtcacctgcgccggcgggccggctcaacaacgtccgcccgatgacgggcgaggagtacctggaatcactgcgggacggccgagaggtctacatctacggcgagcgggtcgacgacgtcaccacgcacctggccttccgcaacagcgtgcgctccatcgcgcggctctacgacgtgctgcacgacccggcgtccgaaggtgtgctgcgggtgcccaccgacaccggcaacggcgggttcacccacccgttcttcaagaccgcccggtcgtcggaggacctggtcgccgcgcgcgaggccatcgtcggctggcagcggctggtgtacgggtggatgggccgcaccccggactacaaggcggcgttcttcggcacgctcgacgccaacgccgagttctacgggccgttcgaggccaacgcccgccgctggtaccgcgacgcccaggaacgggtgctgtacttcaaccacgcgatcgtgcacccgccggtcgaccgggaccggcccgccgaccggaccgccgacatctgcgtgcacgtggaggaggagaccgacagcgggttgatcgtctccggcgccaaggtggtcgcgaccggctccgcgatgaccaacgcgaacctcatcgcgcactacgggcttccggtgcgggacaagaagttcggcctggtgttcacggtcccgatgaactcgcccggcctcaagctcatctgccgcacctcctacgagctgatggtcgcgacgcagggctcgcccttcgactacccgctgtcgagccggctcgacgagaacgactcgatcatgatcttcgaccgggtgctggtgccctgggagaacgtgttcatgtacgacgcgggcgcggccaactccttcgccaccgggtcaggcttcctcgaacgcttcaccttccacggctgcacccgcctcgcggtcaagctggacttcatcgccggctgcgtcatgaaggcggtggaggtcaccggcaccacgcacttccggggcgtgcaggcgcaggtcggcgaagtgctcaactggcgcgacgtgttctggggcctgtccgacgcgatggccaagtcgccgaactcgtgggtcggcggctcggtgcagccgaacctcaactacgggctcgcgtaccgcaccttcatgggcgtgggctacccgcgcatcaaggagatcatccagcagaccctcggcagcgggttgatctacctgaactcctcggccgccgactggaagaaccccgacgtccgcccgtacctcgaccgctacctgcgcggctcgcggggcatccaggcgatcgaccgggtcaagctgctgaagctgctgtgggacgcggtcggcaccgagttcgccggccggcacgagctctacgagcgcaactacggcggcgaccacgagggcatccgggtgcagaccctgcaggcgtaccaggcgaacggccaagccgccgcgctcaagggtttcgccgagcagtgcatgtccgagtacgacctcgacggctggacgaggcccgacctgatcaaccccggcacctaataatactagaggaggttgggacaagatgacgcaggtcgtggaacgtcaggctgatcggctcagcagcagggagtacctggcccgggtcgtgcgcagcgccgggtgggacgccggtctcacctcgtgcaccgacgaggagatcgtccggatgggcgcgagcgcgcgcaccatcgaggagtacctgaagtccgacaagcccatctacggcctgacgcagggcttcggtccgctggtgctgttcgacgccgactcggagctggagcagggcggctcgctgatctcgcacctgggcaccggccagggcgcgccactggccccggaggtgtcgcggctgatcctctggctgcgcatccagaacatgcgcaaggggtactcggcggtctcgccggtgttctggcagaagctcgccgacctgtggaacaaggggttcaccccggcgatcccc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742105_sequence/1
type: Sequence
persistent identity: BBa I742105 sequence
display id: BBa_I742105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acggagaagcagcgaaatgaccatcacgtcacctgcgccggcgggccggctcaacaacgtccgcccgatgacgggcgaggagtacctggaatcactgcgggacggccgagaggtctacatctacggcgagcgggtcgacgacgtcaccacgcacctggccttccgcaacagcgtgcgctccatcgcgcggctctacgacgtgctgcacgacccggcgtccgaaggtgtgctgcgggtgcccaccgacaccggcaacggcgggttcacccacccgttcttcaagaccgcccggtcgtcggaggacctggtcgccgcgcgcgaggccatcgtcggctggcagcggctggtgtacgggtggatgggccgcaccccggactacaaggcggcgttcttcggcacgctcgacgccaacgccgagttctacgggccgttcgaggccaacgcccgccgctggtaccgcgacgcccaggaacgggtgctgtacttcaaccacgcgatcgtgcacccgccggtcgaccgggaccggcccgccgaccggaccgccgacatctgcgtgcacgtggaggaggagaccgacagcgggttgatcgtctccggcgccaaggtggtcgcgaccggctccgcgatgaccaacgcgaacctcatcgcgcactacgggcttccggtgcgggacaagaagttcggcctggtgttcacggtcccgatgaactcgcccggcctcaagctcatctgccgcacctcctacgagctgatggtcgcgacgcagggctcgcccttcgactacccgctgtcgagccggctcgacgagaacgactcgatcatgatcttcgaccgggtgctggtgccctgggagaacgtgttcatgtacgacgcgggcgcggccaactccttcgccaccgggtcaggcttcctcgaacgcttcaccttccacggctgcacccgcctcgcggtcaagctggacttcatcgccggctgcgtcatgaaggcggtggaggtcaccggcaccacgcacttccggggcgtgcaggcgcaggtcggcgaagtgctcaactggcgcgacgtgttctggggcctgtccgacgcgatggccaagtcgccgaactcgtgggtcggcggctcggtgcagccgaacctcaactacgggctcgcgtaccgcaccttcatgggcgtgggctacccgcgcatcaaggagatcatccagcagaccctcggcagcgggttgatctacctgaactcctcggccgccgactggaagaaccccgacgtccgcccgtacctcgaccgctacctgcgcggctcgcggggcatccaggcgatcgaccgggtcaagctgctgaagctgctgtgggacgcggtcggcaccgagttcgccggccggcacgagctctacgagcgcaactacggcggcgaccacgagggcatccgggtgcagaccctgcaggcgtaccaggcgaacggccaagccgccgcgctcaagggtttcgccgagcagtgcatgtccgagtacgacctcgacggctggacgaggcccgacctgatcaaccccggcacctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742106_sequence/1
type: Sequence
persistent identity: BBa I742106 sequence
display id: BBa_I742106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaggttgggacaagatgacgcaggtcgtggaacgtcaggctgatcggctcagcagcagggagtacctggcccgggtcgtgcgcagcgccgggtgggacgccggtctcacctcgtgcaccgacgaggagatcgtccggatgggcgcgagcgcgcgcaccatcgaggagtacctgaagtccgacaagcccatctacggcctgacgcagggcttcggtccgctggtgctgttcgacgccgactcggagctggagcagggcggctcgctgatctcgcacctgggcaccggccagggcgcgccactggccccggaggtgtcgcggctgatcctctggctgcgcatccagaacatgcgcaaggggtactcggcggtctcgccggtgttctggcagaagctcgccgacctgtggaacaaggggttcaccccggcgatcccccggcacggcacggtcagcgcgagcggcgacctgcaaccgctggcgcacgccgcgctcgccttcaccggtgtcggcgaggcgtggacccgggacgccgacggccggtggtccaccgtgccggccgtggacgcgctcgccgcgctgggggcggagccgttcgactggccggtgcgcgaggcgctggcgttcgtcaacgggaccggcgcgagcctcgcggtggctgtgctcaaccaccggtccgccctgcggctggtccgcgcctgcgccgtgctctccgcgcggctggcgaccctgctgggggccaatcccgagcactacgacgtggggcacggtgtcgcgcgcggccaggtcggtcagctgaccgcggcggagtggatccggcaggggctgccccggggcatggtgcgcgacggcagccgcccgctccaggagccgtacagcctgcggtgcgcgccgcaggtgctcggcgcggtgctcgaccagctcgacggcgcgggcgacgtgctggcgcgggaggtcgacggctgccaggacaacccgatcacctacgagggcgagctgctgcacggcggcaacttccacgccatgccggtgggtttcgcctccgaccagatcgggttggccatgcacatggccgcctacctggccgagcgccagctgggtctgctggtcagcccggtgaccaacggcgacctgccgcccatgctcaccccgcgcgccgggcgcggtgccgggctggccggggtgcagatcagcgcgacctcgttcgtctcgcggatccggcagctggtgttccccgcctcgctgaccaccctgccgaccaacggctggaaccaggaccacgtgccgatggcgctcaacggggcgaactcggtgttcgaggcgttggagctcggctggctgacggtcgggtcgctggcggtgggcgtcgcgcagctcgcggccatgaccggccacgccgcggagggcgtctgggcggagctggccgggatctgcccgccgctggacgccgaccgcccgctgggcgccgaggtgcgcgccgcgcgcgacctgctgtccgcgcacgcggaccaactgctcgtcgacgaggcagacgggaaggatttcggataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742107_sequence/1
type: Sequence
persistent identity: BBa I742107 sequence
display id: BBa_I742107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggttcaacaggtgaaactcaaataacaccaacccacatatcagatgaagaagcaaacctcttcgccatgcaactagcaagtgcttcagttcttcccatgattttgaaatcagctcttgaacttgatctcttagaaatcattgctaaagctggacctggtgctcaaatttcacctattgaaattgcttctcagctaccaacaactaaccctgatgcaccagttatgttggaccgaatgttgcgtctcttggcttgttacataatcctcacatgttcagttcgtactcaacaagatggaaaggttcagagactttatggtttggctactgttgctaagtatttggttaagaatgaagatggtgtatccatttctgctcttaatctcatgaatcaggataaagtgctcatggaaagctggtaccacctaaaagatgcagtccttgatgggggcattccattcaacaaggcttatggaatgacagcctttgaataccatggaacagatccaaggtttaacaaggttttcaacaaggggatgtctgatcactctaccatcacaatgaagaaaattcttgagacctacacaggttttgaaggccttaaatctcttgttgatgtaggtggtggtactggagctgtaattaacacgattgtctcaaaatatcccactataaagggtataaattttgatttaccccatgtcattgaagatgctccatcttatccaggagttgagcatgttggtggagacatgtttgtcagtattccaaaggctgatgctgtttttatgaagtggatttgtcatgactggagtgatgagcactgcttgaaatttttgaagaactgctatgaggcactgccagacaatggaaaagtgattgtggcagaatgcatacttccagtggctccagattcaagcctggccacaaaaggtgtggttcacattgatgtgatcatgttggctcataatcctggtgggaaagagagaacacaaaaagagtttgaggatcttgccaaaggtgctggattccaaggtttcaaagtccattgtaatgctttcaacacatacatcatggagtttcttaagaaggtttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742112_sequence/1
type: Sequence
persistent identity: BBa I742112 sequence
display id: BBa_I742112_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcaaatatgaaggccgttggaccaccgtgaaagtggaaattgaagaaggcattgcctgggttattctgaaccgtccggaaaaacgtaacgccatgagcccgaccctgaaccgtgaaatgattgatgtgctggaaaccctggaacaggatccggcggcgggtgttctggttctgaccggtgcgggtgaagcgtggaccgcgggcatggatctgaaagaatatttccgtgaagtggatgcgggtccggaaattctgcaagaaaaaattcgtcgcgaagcgagccagtggcagtggaaactgctgcgtatgtatgcgaaaccgaccattgccatggtgaacggctggtgctttggcggcggttttagcccgctggttgcgtgcgatctggccatttgcgcggatgaagcgacctttggcctgagcgagattaactggggcattccgccgggtaacctggtgagcaaagccatggcggataccgtgggccatcgtcagagcctgtattatatcatgaccggcaaaacctttggtggccagaaagcggcggaaatgggcctggtgaacgaaagcgtgccgctggcccagctgcgtgaagtgaccattgaactggcccgtaacctgctggaaaaaaatccggtggtgctgcgtgcggcgaaacatggctttaaacgttgccgtgaactgacctgggaacagaacgaagattatctgtacgcgaaactggatcagagccgtctgctggataccgaaggcggccgtgaacagggcatgaaacagtttctggatgataaaagcattaaaccgggcctgcaagcgtataaacgttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742114_sequence/1
type: Sequence
persistent identity: BBa I742114 sequence
display id: BBa_I742114_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtagcctggaaccgctggccgaactgccggcacgtctgctggatcgtctggtgcattgggcgcgtgtgcgtccggaacagacctttattgcggcgcgtcaggcgggtggtgattggcgtcgtgtgagctatcgcgaaatgctggatggcgtgcgtgcgattgcgcagggcctgctgtcttatggcctgagcgcggataaaccgctggccctgctgtctggcaacgatattgaacatctgcaagtggcgctgggcgcaatgtatgcgggcattccgtattgcccggtgagcccggcgtatagcctgctgtctcaggattttgcgaaactgcgtcatgtgtgcgatctgctgcaaccgggcctggtttttgtgtctgatgcgccggcatatcagcgtgcgattgatgcggttctgccgccggaaaccccgctgattagcgtgcgtggccaggttccgggccgtacgcagattagctttgcgagcctgctgcataccagcgcgggcaccgaagcggatgcggcgtttaacgcgaccggtccggatagcattgcgaaatttctgtttaccagcggcagcaccaaactgccgaaagcggtgattaccacccagcgtatgctgtgcgcgaaccagcagatgctgctgcaaacctttccggtgtttggcgaagaaccgccggtgctggttgattggctgccgtggaaccacacctttggcggcagccataacgtgggcattgtgctgtataacggcggcaccttttatctggatgatggcaaaccgaccgcccagggctttgcacagacgctgcgcaacctgaaagaaattagcccgaccgcgtatctgaccgtgccgaaaggctgggaagaactggtgaacgcgctggaacaggatgcggcgctgcgtgaacgtttttttgcgcgcatgaaactgtttttttttgcggcggcaggcctgagccagagcgtgtgggatcgtctggatcgtgtggcggaacaacattgtggcgaacgtattcgcatgatggcgggcctgggcatgaccgaagcggcaccgagctgcacctttaccaccggtccgctgtctatggcgggctatattggcctgccggcaccgggttgtgaagtgcgtctggtgccggtggatggcaaatttgaaggccgttttcgtggcccgcatatcatgccgggttattggcgtgcaccggaacagaccgcggaagtgtttgatgaacagggctattattgcagcggtgatgcgatcaaactggccgatccgcatcagccgcattggggcctgatgtttgatggccgtattgcggaagattttaaactgagcagcggcgtgtttgtgtccgtgggcccgctgcgtaatcgtgcggttctggaaggcagcccgtatgtgcaggatctggtgattgcggcaccggatcgtgaatgcctgggcgccctggtttttccgcgtgcgtatgaatgccgtcgtctgagcggcctgaacgcggaagcgtctgatgcggaagtgctgaacagcgcaccggttcgtcagtggtttggcgattggctgcaacgtctgaaccgtgaagcgaccggcaatgcgagccgtctggaatggatcgcggtgctggatgaaccggcgagcattgatcgtggcgaaattaccgataaaggcagcattaaccagcgcgcggtgctgcaatggcgtgcggcgaaagtggaagcgctgtatcgtggcgaagatccgaccattctgcgtgcgggtccgaaaccgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J15001_sequence/1
type: Sequence
persistent identity: BBa J15001 sequence
display id: BBa_J15001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggagg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z