BBa_I742141
1
sam5
sam5 (coumarate hydroxylase) coding sequence
2007-10-22T11:00:00Z
2015-08-31T04:08:03Z
Saccharothrix espanaensis DSM 44229, sequence accession ABC88666.
This is the coding sequence of sam5, encoding 4-coumarate-3-hydroxylase from the bacterium Saccharothrix espanaensis. The enzyme converts p-coumaric acid to caffeic acid. Accession: ABC88666. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis' J. Bacteriol. 188 (7), 2666-2673 (2006). Note: contains a PstI site. This will be removed by site directed mutagenesis in due course but is still present, near the 3' end, in this version.
false
false
_123_
0
837
163
It's complicated
true
Note that this version of the gene contains a PstI site near the 5' end. This will ultimately be removed by site directed mutagenesis.
false
sarah hollingshead
annotation1952768
1
PstI site to be removed
range1952768
1
1420
1425
BBa_I742141_sequence
1
atgaccatcacgtcacctgcgccggcgggccggctcaacaacgtccgcccgatgacgggcgaggagtacctggaatcactgcgggacggccgagaggtctacatctacggcgagcgggtcgacgacgtcaccacgcacctggccttccgcaacagcgtgcgctccatcgcgcggctctacgacgtgctgcacgacccggcgtccgaaggtgtgctgcgggtgcccaccgacaccggcaacggcgggttcacccacccgttcttcaagaccgcccggtcgtcggaggacctggtcgccgcgcgcgaggccatcgtcggctggcagcggctggtgtacgggtggatgggccgcaccccggactacaaggcggcgttcttcggcacgctcgacgccaacgccgagttctacgggccgttcgaggccaacgcccgccgctggtaccgcgacgcccaggaacgggtgctgtacttcaaccacgcgatcgtgcacccgccggtcgaccgggaccggcccgccgaccggaccgccgacatctgcgtgcacgtggaggaggagaccgacagcgggttgatcgtctccggcgccaaggtggtcgcgaccggctccgcgatgaccaacgcgaacctcatcgcgcactacgggcttccggtgcgggacaagaagttcggcctggtgttcacggtcccgatgaactcgcccggcctcaagctcatctgccgcacctcctacgagctgatggtcgcgacgcagggctcgcccttcgactacccgctgtcgagccggctcgacgagaacgactcgatcatgatcttcgaccgggtgctggtgccctgggagaacgtgttcatgtacgacgcgggcgcggccaactccttcgccaccgggtcaggcttcctcgaacgcttcaccttccacggctgcacccgcctcgcggtcaagctggacttcatcgccggctgcgtcatgaaggcggtggaggtcaccggcaccacgcacttccggggcgtgcaggcgcaggtcggcgaagtgctcaactggcgcgacgtgttctggggcctgtccgacgcgatggccaagtcgccgaactcgtgggtcggcggctcggtgcagccgaacctcaactacgggctcgcgtaccgcaccttcatgggcgtgggctacccgcgcatcaaggagatcatccagcagaccctcggcagcgggttgatctacctgaactcctcggccgccgactggaagaaccccgacgtccgcccgtacctcgaccgctacctgcgcggctcgcggggcatccaggcgatcgaccgggtcaagctgctgaagctgctgtgggacgcggtcggcaccgagttcgccggccggcacgagctctacgagcgcaactacggcggcgaccacgagggcatccgggtgcagaccctgcaggcgtaccaggcgaacggccaagccgccgcgctcaagggtttcgccgagcagtgcatgtccgagtacgacctcgacggctggacgaggcccgacctgatcaaccccggcacctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z