BBa_I742147
1
rbs+sam5
sam5 (coumarate hydroxylase) with native rbs.
2007-10-23T11:00:00Z
2015-08-31T04:08:03Z
Saccharothrix espanaensis DSM 44229
Sam5 (4-coumarate-3-hydroxylase) of the bacterium Saccharothrix espanaensis DSM 44229 with its native rbs.
false
false
_123_
0
837
163
It's complicated
true
This was made as a single biobrick, rather than separate rbs and coding sequence, hence the use of blunt ligation in generating this composite part.
false
sarah hollingshead
component1953792
1
BBa_I742145
component1953794
1
BBa_I742141
annotation1953792
1
BBa_I742145
range1953792
1
1
16
annotation1953794
1
BBa_I742141
range1953794
1
17
1558
BBa_I742145
1
BBa_I742145
sam5 native rbs
2007-10-23T11:00:00Z
2015-08-31T04:08:03Z
Saccharothrix espanaensis DSM 44229
This is the native ribosome binding site for sam5 (4-coumarate-3-hydroxylase) of the bacterium Saccharothrix espanaensis.
false
false
_123_
0
837
163
Not in stock
false
This is a virtual part to be added to coding sequence I742141.
false
Chris French
annotation1955475
1
rbs
range1955475
1
3
9
BBa_I742146
1
BBa_I742146
sam8 native rbs
2007-10-23T11:00:00Z
2015-08-31T04:08:03Z
Saccharothrix espanaensis DSM 44229.
Native ribosome binding site of sam8 (tyrosine ammonia lyase) from the bacterium Saccharothrix espanaensis DSM 44229.
false
false
_123_
0
837
163
Not in stock
false
Virtual part to be added to coding sequence I742142.
false
Chris French
BBa_I742142
1
sam8
sam8 (tyrosine-ammonia lyase) coding sequence
2007-10-22T11:00:00Z
2015-08-31T04:08:03Z
Saccharothrix espanaensis DSM 4429. Accession: ABC88666.
This is the coding sequence of sam8, encoding tyrosine ammonia lyase, from the bacterium Saccharothrix espanaensis DSM 4429. The enzyme converts tyrosine to p-coumaric acid. Accession: ABC88666. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis' J. Bacteriol. 188 (7), 2666-2673 (2006)
false
false
_123_
0
837
163
It's complicated
true
No important considerations.
false
sarah hollingshead
BBa_I742143
1
sam8sam5
sam8+sam5 (tyrosine ammonia lyase + coumarate hydroxylase)
2007-10-22T11:00:00Z
2015-08-31T04:08:03Z
Both genes are derived from Saccharothrix espanaensis DSM 4429.
This part consists of the genes sam8 and sam5, encoding tyrosine ammonia lyase and 4-coumarate-3-hydroxylase, from the bacterium Saccharothrix espanaensis DSM 4429. The enzymes acting together are expected to convert tyrosine to caffeic acid. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis' J. Bacteriol. 188 (7), 2666-2673 (2006). Note that there is a PstI site near the 3' end of the sam5 gene in this version. This will be removed by site directed mutagenesis in due course. This construct was prepared to test activity before undertaking the mutagenesis.
false
false
_123_
0
837
163
It's complicated
true
Note that this version of sam5 contains a PstI site near the 3' end. Thus this biobrick was prepared by inserting sam8 (EcoRI-SpeI) upstream of sam5 (EcoRI-XbaI) to avoid cutting with PstI.
false
Chris French
component2220403
1
BBa_I742147
component2220399
1
BBa_I742148
annotation2220403
1
BBa_I742147
range2220403
1
1559
3116
annotation2220399
1
BBa_I742148
range2220399
1
1
1550
BBa_I742141
1
sam5
sam5 (coumarate hydroxylase) coding sequence
2007-10-22T11:00:00Z
2015-08-31T04:08:03Z
Saccharothrix espanaensis DSM 44229, sequence accession ABC88666.
This is the coding sequence of sam5, encoding 4-coumarate-3-hydroxylase from the bacterium Saccharothrix espanaensis. The enzyme converts p-coumaric acid to caffeic acid. Accession: ABC88666. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis' J. Bacteriol. 188 (7), 2666-2673 (2006). Note: contains a PstI site. This will be removed by site directed mutagenesis in due course but is still present, near the 3' end, in this version.
false
false
_123_
0
837
163
It's complicated
true
Note that this version of the gene contains a PstI site near the 5' end. This will ultimately be removed by site directed mutagenesis.
false
sarah hollingshead
annotation1952768
1
PstI site to be removed
range1952768
1
1420
1425
BBa_I742148
1
rbs+sam8
sam8 (tyrosine ammonia lyase) with native rbs
2007-10-23T11:00:00Z
2015-08-31T04:08:03Z
Saccharothrix espanaensis DSM 44229
This is sam8 (tyrosine ammonia lyase) of the bacterium Saccharothrix espanaensis DSM 44229 with its native ribosome binding site.
false
false
_123_
0
837
163
It's complicated
true
This part was made as a single biobrick rather than separate rbs and coding sequence, hence the use of blunt ligation in generating the final sequence.
false
sarah hollingshead
component1953823
1
BBa_I742146
component1953824
1
BBa_I742142
annotation1953823
1
BBa_I742146
range1953823
1
1
14
annotation1953824
1
BBa_I742142
range1953824
1
15
1550
BBa_I742147_sequence
1
acggagaagcagcgaaatgaccatcacgtcacctgcgccggcgggccggctcaacaacgtccgcccgatgacgggcgaggagtacctggaatcactgcgggacggccgagaggtctacatctacggcgagcgggtcgacgacgtcaccacgcacctggccttccgcaacagcgtgcgctccatcgcgcggctctacgacgtgctgcacgacccggcgtccgaaggtgtgctgcgggtgcccaccgacaccggcaacggcgggttcacccacccgttcttcaagaccgcccggtcgtcggaggacctggtcgccgcgcgcgaggccatcgtcggctggcagcggctggtgtacgggtggatgggccgcaccccggactacaaggcggcgttcttcggcacgctcgacgccaacgccgagttctacgggccgttcgaggccaacgcccgccgctggtaccgcgacgcccaggaacgggtgctgtacttcaaccacgcgatcgtgcacccgccggtcgaccgggaccggcccgccgaccggaccgccgacatctgcgtgcacgtggaggaggagaccgacagcgggttgatcgtctccggcgccaaggtggtcgcgaccggctccgcgatgaccaacgcgaacctcatcgcgcactacgggcttccggtgcgggacaagaagttcggcctggtgttcacggtcccgatgaactcgcccggcctcaagctcatctgccgcacctcctacgagctgatggtcgcgacgcagggctcgcccttcgactacccgctgtcgagccggctcgacgagaacgactcgatcatgatcttcgaccgggtgctggtgccctgggagaacgtgttcatgtacgacgcgggcgcggccaactccttcgccaccgggtcaggcttcctcgaacgcttcaccttccacggctgcacccgcctcgcggtcaagctggacttcatcgccggctgcgtcatgaaggcggtggaggtcaccggcaccacgcacttccggggcgtgcaggcgcaggtcggcgaagtgctcaactggcgcgacgtgttctggggcctgtccgacgcgatggccaagtcgccgaactcgtgggtcggcggctcggtgcagccgaacctcaactacgggctcgcgtaccgcaccttcatgggcgtgggctacccgcgcatcaaggagatcatccagcagaccctcggcagcgggttgatctacctgaactcctcggccgccgactggaagaaccccgacgtccgcccgtacctcgaccgctacctgcgcggctcgcggggcatccaggcgatcgaccgggtcaagctgctgaagctgctgtgggacgcggtcggcaccgagttcgccggccggcacgagctctacgagcgcaactacggcggcgaccacgagggcatccgggtgcagaccctgcaggcgtaccaggcgaacggccaagccgccgcgctcaagggtttcgccgagcagtgcatgtccgagtacgacctcgacggctggacgaggcccgacctgatcaaccccggcacctaataa
BBa_I742146_sequence
1
gaggttgggacaag
BBa_I742141_sequence
1
atgaccatcacgtcacctgcgccggcgggccggctcaacaacgtccgcccgatgacgggcgaggagtacctggaatcactgcgggacggccgagaggtctacatctacggcgagcgggtcgacgacgtcaccacgcacctggccttccgcaacagcgtgcgctccatcgcgcggctctacgacgtgctgcacgacccggcgtccgaaggtgtgctgcgggtgcccaccgacaccggcaacggcgggttcacccacccgttcttcaagaccgcccggtcgtcggaggacctggtcgccgcgcgcgaggccatcgtcggctggcagcggctggtgtacgggtggatgggccgcaccccggactacaaggcggcgttcttcggcacgctcgacgccaacgccgagttctacgggccgttcgaggccaacgcccgccgctggtaccgcgacgcccaggaacgggtgctgtacttcaaccacgcgatcgtgcacccgccggtcgaccgggaccggcccgccgaccggaccgccgacatctgcgtgcacgtggaggaggagaccgacagcgggttgatcgtctccggcgccaaggtggtcgcgaccggctccgcgatgaccaacgcgaacctcatcgcgcactacgggcttccggtgcgggacaagaagttcggcctggtgttcacggtcccgatgaactcgcccggcctcaagctcatctgccgcacctcctacgagctgatggtcgcgacgcagggctcgcccttcgactacccgctgtcgagccggctcgacgagaacgactcgatcatgatcttcgaccgggtgctggtgccctgggagaacgtgttcatgtacgacgcgggcgcggccaactccttcgccaccgggtcaggcttcctcgaacgcttcaccttccacggctgcacccgcctcgcggtcaagctggacttcatcgccggctgcgtcatgaaggcggtggaggtcaccggcaccacgcacttccggggcgtgcaggcgcaggtcggcgaagtgctcaactggcgcgacgtgttctggggcctgtccgacgcgatggccaagtcgccgaactcgtgggtcggcggctcggtgcagccgaacctcaactacgggctcgcgtaccgcaccttcatgggcgtgggctacccgcgcatcaaggagatcatccagcagaccctcggcagcgggttgatctacctgaactcctcggccgccgactggaagaaccccgacgtccgcccgtacctcgaccgctacctgcgcggctcgcggggcatccaggcgatcgaccgggtcaagctgctgaagctgctgtgggacgcggtcggcaccgagttcgccggccggcacgagctctacgagcgcaactacggcggcgaccacgagggcatccgggtgcagaccctgcaggcgtaccaggcgaacggccaagccgccgcgctcaagggtttcgccgagcagtgcatgtccgagtacgacctcgacggctggacgaggcccgacctgatcaaccccggcacctaataa
BBa_I742143_sequence
1
gaggttgggacaagatgacgcaggtcgtggaacgtcaggctgatcggctcagcagcagggagtacctggcccgggtcgtgcgcagcgccgggtgggacgccggtctcacctcgtgcaccgacgaggagatcgtccggatgggcgcgagcgcgcgcaccatcgaggagtacctgaagtccgacaagcccatctacggcctgacgcagggcttcggtccgctggtgctgttcgacgccgactcggagctggagcagggcggctcgctgatctcgcacctgggcaccggccagggcgcgccactggccccggaggtgtcgcggctgatcctctggctgcgcatccagaacatgcgcaaggggtactcggcggtctcgccggtgttctggcagaagctcgccgacctgtggaacaaggggttcaccccggcgatcccccggcacggcacggtcagcgcgagcggcgacctgcaaccgctggcgcacgccgcgctcgccttcaccggtgtcggcgaggcgtggacccgggacgccgacggccggtggtccaccgtgccggccgtggacgcgctcgccgcgctgggggcggagccgttcgactggccggtgcgcgaggcgctggcgttcgtcaacgggaccggcgcgagcctcgcggtggctgtgctcaaccaccggtccgccctgcggctggtccgcgcctgcgccgtgctctccgcgcggctggcgaccctgctgggggccaatcccgagcactacgacgtggggcacggtgtcgcgcgcggccaggtcggtcagctgaccgcggcggagtggatccggcaggggctgccccggggcatggtgcgcgacggcagccgcccgctccaggagccgtacagcctgcggtgcgcgccgcaggtgctcggcgcggtgctcgaccagctcgacggcgcgggcgacgtgctggcgcgggaggtcgacggctgccaggacaacccgatcacctacgagggcgagctgctgcacggcggcaacttccacgccatgccggtgggtttcgcctccgaccagatcgggttggccatgcacatggccgcctacctggccgagcgccagctgggtctgctggtcagcccggtgaccaacggcgacctgccgcccatgctcaccccgcgcgccgggcgcggtgccgggctggccggggtgcagatcagcgcgacctcgttcgtctcgcggatccggcagctggtgttccccgcctcgctgaccaccctgccgaccaacggctggaaccaggaccacgtgccgatggcgctcaacggggcgaactcggtgttcgaggcgttggagctcggctggctgacggtcgggtcgctggcggtgggcgtcgcgcagctcgcggccatgaccggccacgccgcggagggcgtctgggcggagctggccgggatctgcccgccgctggacgccgaccgcccgctgggcgccgaggtgcgcgccgcgcgcgacctgctgtccgcgcacgcggaccaactgctcgtcgacgaggcagacgggaaggatttcggataataatactagagacggagaagcagcgaaatgaccatcacgtcacctgcgccggcgggccggctcaacaacgtccgcccgatgacgggcgaggagtacctggaatcactgcgggacggccgagaggtctacatctacggcgagcgggtcgacgacgtcaccacgcacctggccttccgcaacagcgtgcgctccatcgcgcggctctacgacgtgctgcacgacccggcgtccgaaggtgtgctgcgggtgcccaccgacaccggcaacggcgggttcacccacccgttcttcaagaccgcccggtcgtcggaggacctggtcgccgcgcgcgaggccatcgtcggctggcagcggctggtgtacgggtggatgggccgcaccccggactacaaggcggcgttcttcggcacgctcgacgccaacgccgagttctacgggccgttcgaggccaacgcccgccgctggtaccgcgacgcccaggaacgggtgctgtacttcaaccacgcgatcgtgcacccgccggtcgaccgggaccggcccgccgaccggaccgccgacatctgcgtgcacgtggaggaggagaccgacagcgggttgatcgtctccggcgccaaggtggtcgcgaccggctccgcgatgaccaacgcgaacctcatcgcgcactacgggcttccggtgcgggacaagaagttcggcctggtgttcacggtcccgatgaactcgcccggcctcaagctcatctgccgcacctcctacgagctgatggtcgcgacgcagggctcgcccttcgactacccgctgtcgagccggctcgacgagaacgactcgatcatgatcttcgaccgggtgctggtgccctgggagaacgtgttcatgtacgacgcgggcgcggccaactccttcgccaccgggtcaggcttcctcgaacgcttcaccttccacggctgcacccgcctcgcggtcaagctggacttcatcgccggctgcgtcatgaaggcggtggaggtcaccggcaccacgcacttccggggcgtgcaggcgcaggtcggcgaagtgctcaactggcgcgacgtgttctggggcctgtccgacgcgatggccaagtcgccgaactcgtgggtcggcggctcggtgcagccgaacctcaactacgggctcgcgtaccgcaccttcatgggcgtgggctacccgcgcatcaaggagatcatccagcagaccctcggcagcgggttgatctacctgaactcctcggccgccgactggaagaaccccgacgtccgcccgtacctcgaccgctacctgcgcggctcgcggggcatccaggcgatcgaccgggtcaagctgctgaagctgctgtgggacgcggtcggcaccgagttcgccggccggcacgagctctacgagcgcaactacggcggcgaccacgagggcatccgggtgcagaccctgcaggcgtaccaggcgaacggccaagccgccgcgctcaagggtttcgccgagcagtgcatgtccgagtacgacctcgacggctggacgaggcccgacctgatcaaccccggcacctaataa
BBa_I742145_sequence
1
acggagaagcagcgaa
BBa_I742148_sequence
1
gaggttgggacaagatgacgcaggtcgtggaacgtcaggctgatcggctcagcagcagggagtacctggcccgggtcgtgcgcagcgccgggtgggacgccggtctcacctcgtgcaccgacgaggagatcgtccggatgggcgcgagcgcgcgcaccatcgaggagtacctgaagtccgacaagcccatctacggcctgacgcagggcttcggtccgctggtgctgttcgacgccgactcggagctggagcagggcggctcgctgatctcgcacctgggcaccggccagggcgcgccactggccccggaggtgtcgcggctgatcctctggctgcgcatccagaacatgcgcaaggggtactcggcggtctcgccggtgttctggcagaagctcgccgacctgtggaacaaggggttcaccccggcgatcccccggcacggcacggtcagcgcgagcggcgacctgcaaccgctggcgcacgccgcgctcgccttcaccggtgtcggcgaggcgtggacccgggacgccgacggccggtggtccaccgtgccggccgtggacgcgctcgccgcgctgggggcggagccgttcgactggccggtgcgcgaggcgctggcgttcgtcaacgggaccggcgcgagcctcgcggtggctgtgctcaaccaccggtccgccctgcggctggtccgcgcctgcgccgtgctctccgcgcggctggcgaccctgctgggggccaatcccgagcactacgacgtggggcacggtgtcgcgcgcggccaggtcggtcagctgaccgcggcggagtggatccggcaggggctgccccggggcatggtgcgcgacggcagccgcccgctccaggagccgtacagcctgcggtgcgcgccgcaggtgctcggcgcggtgctcgaccagctcgacggcgcgggcgacgtgctggcgcgggaggtcgacggctgccaggacaacccgatcacctacgagggcgagctgctgcacggcggcaacttccacgccatgccggtgggtttcgcctccgaccagatcgggttggccatgcacatggccgcctacctggccgagcgccagctgggtctgctggtcagcccggtgaccaacggcgacctgccgcccatgctcaccccgcgcgccgggcgcggtgccgggctggccggggtgcagatcagcgcgacctcgttcgtctcgcggatccggcagctggtgttccccgcctcgctgaccaccctgccgaccaacggctggaaccaggaccacgtgccgatggcgctcaacggggcgaactcggtgttcgaggcgttggagctcggctggctgacggtcgggtcgctggcggtgggcgtcgcgcagctcgcggccatgaccggccacgccgcggagggcgtctgggcggagctggccgggatctgcccgccgctggacgccgaccgcccgctgggcgccgaggtgcgcgccgcgcgcgacctgctgtccgcgcacgcggaccaactgctcgtcgacgaggcagacgggaaggatttcggataataa
BBa_I742142_sequence
1
atgacgcaggtcgtggaacgtcaggctgatcggctcagcagcagggagtacctggcccgggtcgtgcgcagcgccgggtgggacgccggtctcacctcgtgcaccgacgaggagatcgtccggatgggcgcgagcgcgcgcaccatcgaggagtacctgaagtccgacaagcccatctacggcctgacgcagggcttcggtccgctggtgctgttcgacgccgactcggagctggagcagggcggctcgctgatctcgcacctgggcaccggccagggcgcgccactggccccggaggtgtcgcggctgatcctctggctgcgcatccagaacatgcgcaaggggtactcggcggtctcgccggtgttctggcagaagctcgccgacctgtggaacaaggggttcaccccggcgatcccccggcacggcacggtcagcgcgagcggcgacctgcaaccgctggcgcacgccgcgctcgccttcaccggtgtcggcgaggcgtggacccgggacgccgacggccggtggtccaccgtgccggccgtggacgcgctcgccgcgctgggggcggagccgttcgactggccggtgcgcgaggcgctggcgttcgtcaacgggaccggcgcgagcctcgcggtggctgtgctcaaccaccggtccgccctgcggctggtccgcgcctgcgccgtgctctccgcgcggctggcgaccctgctgggggccaatcccgagcactacgacgtggggcacggtgtcgcgcgcggccaggtcggtcagctgaccgcggcggagtggatccggcaggggctgccccggggcatggtgcgcgacggcagccgcccgctccaggagccgtacagcctgcggtgcgcgccgcaggtgctcggcgcggtgctcgaccagctcgacggcgcgggcgacgtgctggcgcgggaggtcgacggctgccaggacaacccgatcacctacgagggcgagctgctgcacggcggcaacttccacgccatgccggtgggtttcgcctccgaccagatcgggttggccatgcacatggccgcctacctggccgagcgccagctgggtctgctggtcagcccggtgaccaacggcgacctgccgcccatgctcaccccgcgcgccgggcgcggtgccgggctggccggggtgcagatcagcgcgacctcgttcgtctcgcggatccggcagctggtgttccccgcctcgctgaccaccctgccgaccaacggctggaaccaggaccacgtgccgatggcgctcaacggggcgaactcggtgttcgaggcgttggagctcggctggctgacggtcgggtcgctggcggtgggcgtcgcgcagctcgcggccatgaccggccacgccgcggagggcgtctgggcggagctggccgggatctgcccgccgctggacgccgaccgcccgctgggcgccgaggtgcgcgccgcgcgcgacctgctgtccgcgcacgcggaccaactgctcgtcgacgaggcagacgggaaggatttcggataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z