LOCUS       BBa_I742144             2158 bp    DNA     linear       15-May-2024
DEFINITION  Plac+lacZ+sam8 (tyrosine ammonia lyase)
ACCESSION   BBa_I742144
VERSION     BBa_I742144.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     misc_feature    248..285
                     /label=CAP binding site
     misc_feature    332..352
                     /label=LacI binding site
     RBS             359..362
                     /label=rbs
     CDS             370..600
                     /label=lacZ'
     promoter        320..325
                     /label=-10
     promoter        297..302
                     /label=-35
     misc_feature    1..600
                     /label=BBa_J33207
     RBS             609..622
                     /label=BBa_I742146
     CDS             623..2158
                     /label=BBa_I742142
     misc_feature    609..2158
                     /label=BBa_I742148
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
      181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
      361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga
      601 tactagagga ggttgggaca agatgacgca ggtcgtggaa cgtcaggctg atcggctcag
      661 cagcagggag tacctggccc gggtcgtgcg cagcgccggg tgggacgccg gtctcacctc
      721 gtgcaccgac gaggagatcg tccggatggg cgcgagcgcg cgcaccatcg aggagtacct
      781 gaagtccgac aagcccatct acggcctgac gcagggcttc ggtccgctgg tgctgttcga
      841 cgccgactcg gagctggagc agggcggctc gctgatctcg cacctgggca ccggccaggg
      901 cgcgccactg gccccggagg tgtcgcggct gatcctctgg ctgcgcatcc agaacatgcg
      961 caaggggtac tcggcggtct cgccggtgtt ctggcagaag ctcgccgacc tgtggaacaa
     1021 ggggttcacc ccggcgatcc cccggcacgg cacggtcagc gcgagcggcg acctgcaacc
     1081 gctggcgcac gccgcgctcg ccttcaccgg tgtcggcgag gcgtggaccc gggacgccga
     1141 cggccggtgg tccaccgtgc cggccgtgga cgcgctcgcc gcgctggggg cggagccgtt
     1201 cgactggccg gtgcgcgagg cgctggcgtt cgtcaacggg accggcgcga gcctcgcggt
     1261 ggctgtgctc aaccaccggt ccgccctgcg gctggtccgc gcctgcgccg tgctctccgc
     1321 gcggctggcg accctgctgg gggccaatcc cgagcactac gacgtggggc acggtgtcgc
     1381 gcgcggccag gtcggtcagc tgaccgcggc ggagtggatc cggcaggggc tgccccgggg
     1441 catggtgcgc gacggcagcc gcccgctcca ggagccgtac agcctgcggt gcgcgccgca
     1501 ggtgctcggc gcggtgctcg accagctcga cggcgcgggc gacgtgctgg cgcgggaggt
     1561 cgacggctgc caggacaacc cgatcaccta cgagggcgag ctgctgcacg gcggcaactt
     1621 ccacgccatg ccggtgggtt tcgcctccga ccagatcggg ttggccatgc acatggccgc
     1681 ctacctggcc gagcgccagc tgggtctgct ggtcagcccg gtgaccaacg gcgacctgcc
     1741 gcccatgctc accccgcgcg ccgggcgcgg tgccgggctg gccggggtgc agatcagcgc
     1801 gacctcgttc gtctcgcgga tccggcagct ggtgttcccc gcctcgctga ccaccctgcc
     1861 gaccaacggc tggaaccagg accacgtgcc gatggcgctc aacggggcga actcggtgtt
     1921 cgaggcgttg gagctcggct ggctgacggt cgggtcgctg gcggtgggcg tcgcgcagct
     1981 cgcggccatg accggccacg ccgcggaggg cgtctgggcg gagctggccg ggatctgccc
     2041 gccgctggac gccgaccgcc cgctgggcgc cgaggtgcgc gccgcgcgcg acctgctgtc
     2101 cgcgcacgcg gaccaactgc tcgtcgacga ggcagacggg aaggatttcg gataataa
//