BBa_I744003
1
BBa_I744003
protein generator for biosynthesis of vanillin
2007-05-25T11:00:00Z
2015-08-31T04:08:03Z
see Biobrick I744002
Protein generator version of BBa-I744002.
strong ribosomal binding site, promoter, and strong terminator
true
false
_147_
0
678
75
Discontinued
false
strong ribosome binding site..constitutive promoter...
false
Beth Beason
component1933876
1
BBa_J23100
component1933885
1
BBa_B0011
component1933878
1
BBa_B0030
component1933881
1
BBa_B0012
component1933880
1
BBa_I744002
annotation1933881
1
BBa_B0012
range1933881
1
1475
1515
annotation1933885
1
BBa_B0011
range1933885
1
1524
1569
annotation1933880
1
BBa_I744002
range1933880
1
67
1466
annotation1933878
1
BBa_B0030
range1933878
1
44
58
annotation1933876
1
BBa_J23100
range1933876
1
1
35
BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation1683
1
stem_loop
range1683
1
13
35
annotation7019
1
BBa_B0011
range7019
1
1
46
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_I744002
1
BBa_I744002
feruloyl-CoA hydratase for biosynthesis of vanillin
2007-05-25T11:00:00Z
2015-08-31T04:08:03Z
GenBank Accession #: AJ536325
*include lit. ref.
one of two enzymes...
true
false
_147_
0
678
75
Discontinued
false
changed stop codon from TGA to TAATAA BioBricks standard end
false
Beth Beason
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_I744002_sequence
1
agtgtctgccgtgctggcttagctgttgccgttccacttggcggcgggttttattgaacgcagccacggcacctgaccctgcgcaattactactggatcgcgactaccaggacggtggggcgaagaccgccgcgcgggagtgggcccaaggtttcatcatgaacatggagtcggggttcaccgagggtcgcgtcggtttcggcctcgatgtgcgcgggctgctgggggtcaagctcgactcctcgccggaccgcagcggcaccgagctgctgccggtgtcggccagcgacaagcgcgccgtggatgaatactcgcggctggcgcccaccgccaaactgcgctttgcccggaccacggtgaagaccggcgatgtctcgatcttcctgccgttcgcctttgccagcccgtcgcgcctgctgccgcagacctttcgcggcaccaccctgagctccacggacatcgacggcctgaccctcaataccggctatatcgaccgcatcaacaagcgcgactccaccgactaccaggccatgaccattgcctcgcccaaccggcggttcaatggcacggccagcacctcgcacctggcgtacctggggggcgattaccaggtcaacaaggcgctcagcctgcgcgcctatcacgccgaggtggccgacctctaccagcaggacactctggccctgctgcacaacctgcccctgggcgatggcgtgctgagcagcgacctgcgcagttttttcagccgggaagacggcagtgccaaggccggccgggtcgacaaccgcaacctctcggcgctgtttggctacaagttcggcgggcaccgggtgagcctgggctacatgcattccagtggcgacacggccacgccctacatctccggcaccgagttgatgggcatgagcgagctgaccatgagttcggacttactcaatgccaaggagcgcacctggcaagccatctacgactatgacttgccgctccgcgtgcccgggctcaagagtcggctgcgttacgtgcgcggcgaccatatcgagctggcggccttcaatgccagccagcgcaaggagcgcgagtttcagatggagttggggtacgtgctgcagagcggcccgctgaagaacctcgggctggtggcgcgcaagtcgatctatcgcaatgacttcccggcgggcgcggcgtttcgcgatgaaaaccagacccgcttcctggtgctctacaccttgccgatctggtaagcccgccagcagcctggctgcctcagcgtgccggccctgtgccggcaccttggcttcagtcctggtacaccttcttcagcaggcgcagcagctcttcgcgttcctcggcgctcaaggccgaggtggcgtcgacgtcgctttgcgcggcgatctgcttga
BBa_I744003_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagagagtgtctgccgtgctggcttagctgttgccgttccacttggcggcgggttttattgaacgcagccacggcacctgaccctgcgcaattactactggatcgcgactaccaggacggtggggcgaagaccgccgcgcgggagtgggcccaaggtttcatcatgaacatggagtcggggttcaccgagggtcgcgtcggtttcggcctcgatgtgcgcgggctgctgggggtcaagctcgactcctcgccggaccgcagcggcaccgagctgctgccggtgtcggccagcgacaagcgcgccgtggatgaatactcgcggctggcgcccaccgccaaactgcgctttgcccggaccacggtgaagaccggcgatgtctcgatcttcctgccgttcgcctttgccagcccgtcgcgcctgctgccgcagacctttcgcggcaccaccctgagctccacggacatcgacggcctgaccctcaataccggctatatcgaccgcatcaacaagcgcgactccaccgactaccaggccatgaccattgcctcgcccaaccggcggttcaatggcacggccagcacctcgcacctggcgtacctggggggcgattaccaggtcaacaaggcgctcagcctgcgcgcctatcacgccgaggtggccgacctctaccagcaggacactctggccctgctgcacaacctgcccctgggcgatggcgtgctgagcagcgacctgcgcagttttttcagccgggaagacggcagtgccaaggccggccgggtcgacaaccgcaacctctcggcgctgtttggctacaagttcggcgggcaccgggtgagcctgggctacatgcattccagtggcgacacggccacgccctacatctccggcaccgagttgatgggcatgagcgagctgaccatgagttcggacttactcaatgccaaggagcgcacctggcaagccatctacgactatgacttgccgctccgcgtgcccgggctcaagagtcggctgcgttacgtgcgcggcgaccatatcgagctggcggccttcaatgccagccagcgcaaggagcgcgagtttcagatggagttggggtacgtgctgcagagcggcccgctgaagaacctcgggctggtggcgcgcaagtcgatctatcgcaatgacttcccggcgggcgcggcgtttcgcgatgaaaaccagacccgcttcctggtgctctacaccttgccgatctggtaagcccgccagcagcctggctgcctcagcgtgccggccctgtgccggcaccttggcttcagtcctggtacaccttcttcagcaggcgcagcagctcttcgcgttcctcggcgctcaaggccgaggtggcgtcgacgtcgctttgcgcggcgatctgcttgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z