LOCUS       BBa_I744102             1831 bp    DNA     linear       29-Nov-2024
DEFINITION  Tetracycline sensor with medium-strength promoter
ACCESSION   BBa_I744102
VERSION     BBa_I744102.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23114
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..721
                     /label=BBa_C0040
     stem_loop       766..809
                     /label=stem_loop
     misc_feature    755..834
                     /label=BBa_B0010
     terminator      755..834
                     /label=BBa_B0010
     stem_loop       850..869
                     /label=T7 TE
     misc_feature    876..876
                     /label=stop
     polyA_site      870..883
                     /label=polya
     misc_feature    843..883
                     /label=BBa_B0012
     terminator      843..883
                     /label=BBa_B0012
     misc_feature    892..910
                     /label=TetR 1
     misc_feature    892..945
                     /label=BBa_R0040
     promoter        911..916
                     /label=-35
     misc_feature    917..935
                     /label=TetR 2
     promoter        934..939
                     /label=-10
     promoter        892..945
                     /label=BBa_R0040
     misc_feature    44..945
                     /label=BBa_Q04400
     misc_feature    958..961
                     /label=conserved
     RBS             954..965
                     /label=BBa_B0034
     CDS             972..1694
                     /label=BBa_E0030
     stem_loop       1714..1757
                     /label=stem_loop
     misc_feature    1703..1782
                     /label=BBa_B0010
     terminator      1703..1782
                     /label=BBa_B0010
     stem_loop       1798..1817
                     /label=T7 TE
     misc_feature    1824..1824
                     /label=stop
     polyA_site      1818..1831
                     /label=polya
     misc_feature    1791..1831
                     /label=BBa_B0012
     terminator      1791..1831
                     /label=BBa_B0012
     misc_feature    954..1831
                     /label=BBa_E0430
ORIGIN
        1 tttatggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca
      901 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg
      961 agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1021 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     1081 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     1141 cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc cgctaccccg
     1201 accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1261 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     1321 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1381 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     1441 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     1501 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1561 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg
     1621 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     1681 tgtacaagta ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1741 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1801 tcaccttcgg gtgggccttt ctgcgtttat a
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