LOCUS BBa_I744203 2831 bp DNA linear 22-Nov-2024 DEFINITION Tc sensor and cl lambda generator (strong promoter) ACCESSION BBa_I744203 VERSION BBa_I744203.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23106 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 65..681 /label=tetR misc_feature 682..715 /label=SsrA misc_feature 722..746 /label=Help:Barcodes CDS 62..721 /label=BBa_C0040 stem_loop 766..809 /label=stem_loop misc_feature 755..834 /label=BBa_B0010 terminator 755..834 /label=BBa_B0010 stem_loop 850..869 /label=T7 TE misc_feature 876..876 /label=stop polyA_site 870..883 /label=polya misc_feature 843..883 /label=BBa_B0012 terminator 843..883 /label=BBa_B0012 misc_feature 892..910 /label=TetR 1 misc_feature 892..945 /label=BBa_R0040 promoter 911..916 /label=-35 misc_feature 917..935 /label=TetR 2 promoter 934..939 /label=-10 promoter 892..945 /label=BBa_R0040 misc_feature 44..945 /label=BBa_Q04400 misc_feature 958..961 /label=conserved RBS 954..965 /label=BBa_B0034 CDS 972..1694 /label=BBa_E0030 stem_loop 1714..1757 /label=stem_loop misc_feature 1703..1782 /label=BBa_B0010 terminator 1703..1782 /label=BBa_B0010 stem_loop 1798..1817 /label=T7 TE misc_feature 1824..1824 /label=stop polyA_site 1818..1831 /label=polya misc_feature 1791..1831 /label=BBa_B0012 terminator 1791..1831 /label=BBa_B0012 misc_feature 954..1831 /label=BBa_E0430 misc_feature 1840..1858 /label=TetR 1 misc_feature 1840..1893 /label=BBa_R0040 promoter 1859..1864 /label=-35 misc_feature 1865..1883 /label=TetR 2 promoter 1882..1887 /label=-10 promoter 1840..1893 /label=BBa_R0040 misc_feature 1906..1909 /label=conserved RBS 1902..1913 /label=BBa_B0034 CDS 1923..2630 /label=cI lambda misc_feature 2631..2663 /label=LVA misc_feature 2670..2694 /label=Help:Barcodes CDS 1920..2669 /label=BBa_C0051 stem_loop 2714..2757 /label=stem_loop misc_feature 2703..2782 /label=BBa_B0010 terminator 2703..2782 /label=BBa_B0010 stem_loop 2798..2817 /label=T7 TE misc_feature 2824..2824 /label=stop polyA_site 2818..2831 /label=polya misc_feature 2791..2831 /label=BBa_B0012 terminator 2791..2831 /label=BBa_B0012 misc_feature 1902..2831 /label=BBa_P0451 ORIGIN 1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta 61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca 901 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg 961 agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 1021 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 1081 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 1141 cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc cgctaccccg 1201 accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 1261 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 1321 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 1381 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca 1441 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg 1501 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 1561 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg 1621 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 1681 tgtacaagta ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 1741 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 1801 tcaccttcgg gtgggccttt ctgcgtttat atactagagt ccctatcagt gatagagatt 1861 gacatcccta tcagtgatag agatactgag cactactaga gaaagaggag aaatactaga 1921 tgagcacaaa aaagaaacca ttaacacaag agcagcttga ggacgcacgt cgccttaaag 1981 caatttatga aaaaaagaaa aatgaacttg gcttatccca ggaatctgtc gcagacaaga 2041 tggggatggg gcagtcaggc gttggtgctt tatttaatgg catcaatgca ttaaatgctt 2101 ataacgccgc attgcttgca aaaattctca aagttagcgt tgaagaattt agcccttcaa 2161 tcgccagaga aatctacgag atgtatgaag cggttagtat gcagccgtca cttagaagtg 2221 agtatgagta ccctgttttt tctcatgttc aggcagggat gttctcacct gagcttagaa 2281 cctttaccaa aggtgatgcg gagagatggg taagcacaac caaaaaagcc agtgattctg 2341 cattctggct tgaggttgaa ggtaattcca tgaccgcacc aacaggctcc aagccaagct 2401 ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc tgttgagcca ggtgatttct 2461 gcatagccag acttgggggt gatgagttta ccttcaagaa actgatcagg gatagcggtc 2521 aggtgttttt acaaccacta aacccacagt acccaatgat cccatgcaat gagagttgtt 2581 ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga gacgtttggc gctgcaaacg 2641 acgaaaacta cgctttagta gcttaataac gctgatagtg ctagtgtaga tcgctactag 2701 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 2761 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 2821 ctgcgtttat a //