##gff-version 3
##sequence-region BBa_I744204 1 2816
BBa_I744204	.	promoter	1859	1912	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I744204	.	engineered_region	1859	1912	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I744204	.	promoter	1878	1883	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I744204	.	non_covalent_binding_site	1859	1877	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I744204	.	promoter	1901	1906	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I744204	.	non_covalent_binding_site	1884	1902	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I744204	.	engineered_region	1921	2816	.	+	0	ID=BBa_P0455;Name=BBa_P0455
BBa_I744204	.	CDS	1939	2688	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_P0455
BBa_I744204	.	tag	2650	2682	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I744204	.	engineered_tag	2689	2713	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I744204	.	CDS	1942	2649	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I744204	.	terminator	2722	2762	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0455
BBa_I744204	.	stem_loop	2729	2748	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I744204	.	stop_codon	2755	2755	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I744204	.	polyA_site	2749	2762	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I744204	.	engineered_region	2722	2762	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I744204	.	terminator	2771	2816	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_P0455
BBa_I744204	.	engineered_region	2771	2816	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I744204	.	stem_loop	2783	2805	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I744204	.	ribosome_entry_site	1921	1932	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0455
BBa_I744204	.	conserved_region	1925	1928	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I744204	.	engineered_region	973	1850	.	+	0	ID=BBa_E0430;Name=BBa_E0430
BBa_I744204	.	terminator	1722	1801	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0430
BBa_I744204	.	stem_loop	1733	1776	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I744204	.	engineered_region	1722	1801	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I744204	.	ribosome_entry_site	973	984	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0430
BBa_I744204	.	conserved_region	977	980	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I744204	.	terminator	1810	1850	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0430
BBa_I744204	.	stem_loop	1817	1836	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I744204	.	stop_codon	1843	1843	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I744204	.	polyA_site	1837	1850	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I744204	.	engineered_region	1810	1850	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I744204	.	CDS	991	1713	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_E0430
BBa_I744204	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I744204	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I744204	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I744204	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I744204	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I744204	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I744204	.	engineered_region	63	964	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400
BBa_I744204	.	terminator	774	853	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_I744204	.	stem_loop	785	828	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I744204	.	engineered_region	774	853	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I744204	.	CDS	81	740	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_I744204	.	tag	701	734	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I744204	.	engineered_tag	741	765	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I744204	.	CDS	84	700	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I744204	.	promoter	911	964	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_I744204	.	engineered_region	911	964	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I744204	.	promoter	930	935	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I744204	.	non_covalent_binding_site	911	929	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I744204	.	promoter	953	958	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I744204	.	non_covalent_binding_site	936	954	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I744204	.	terminator	862	902	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_I744204	.	stem_loop	869	888	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I744204	.	stop_codon	895	895	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I744204	.	polyA_site	889	902	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I744204	.	engineered_region	862	902	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I744204	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_I744204	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I744204
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaaca
acccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcg
acgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagatttttt
acgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcct
acagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattat
atgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaag
ggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagcca
gccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacg
acgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtga
tagagatactgagcactactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtgg
tgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccac
ctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttc
ggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaag
gctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcga
caccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtac
aactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccaca
acatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcc
cgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggag
ttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtga
tagagatactgagcactactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagc
ttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcaga
caagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattg
cttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcgg
ttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctga
gcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggctt
gaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgacc
ctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcag
ggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtg
gggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaat
aacgctgatagtgctagtgtagatcgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatac
tagagagagaatataaaaagccagattattaatccggcttttttattattt