LOCUS BBa_C0620 1656 bp DNA linear 27-Dec-2024 DEFINITION Tsr ACCESSION BBa_C0620 VERSION BBa_C0620.1 FEATURES Location/Qualifiers ORIGIN 1 atgttaaaac gtatcaaaat tgtgaccagc ttactgctgg ttttggccgt ttttggcctt 61 ttacaactga catcaggcgg tctgttcttt aatgccttaa agaatgacaa agaaaatttc 121 actgttttac aaaccattcg ccagcagcaa tccacgctga atggcagctg ggtcgcgttg 181 ttgcagacgc gtaacaccct caaccgcgcg ggtatccgct acatgatgga tcagaataat 241 attggtagcg gttcaaccgt tgctgagctg atggagagtg ccagtatttc gctgaaacag 301 gcggaaaaaa actgggcgga ttacgaagcg ttgccgcgtg acccgcgtca gagcaccgcc 361 gcagcggcag agatcaaacg taattacgat atttatcaca atgcgctggc ggagctgatc 421 caactgttag gtgcaggcaa aatcaacgag ttctttgatc agccgaccca gggatatcag 481 gacggtttcg agaagcagta tgtggcttac atggagcaaa acgatcggct ccatgatatc 541 gccgtcagcg ataacaatgc ctcctacagc caggcgatgt ggattctggt gggcgtgatg 601 atcgtcgtac tggcggtcat cttcgccgtc tggttcggta ttaaagcctc gctggtagcg 661 ccaatgaatc gcctgattga cagcattcgt catattgcag gcggcgatct ggtgaaaccg 721 attgaggtgg atggctctaa tgagatgggg caactggcag agagtttgcg ccatatgcag 781 ggagagctga tgcgtaccgt cggtgatgtg cgcaacgggg ccaatgccat ctatagcggt 841 gccagcgaaa tcgccaccgg caataacgat ctctcttcgc gcaccgagca acaggccgct 901 tcgctggaag agacggcagc cagcatggag caactgaccg caacggtgaa gcagaacgcc 961 gaaaatgcgc gccaggccag ccatctggcg ttaagtgctt ctgaaacggc gcaacgcggc 1021 ggtaaagtgg tagataacgt ggtgcagact atgcgcgata tctccaccag ttcgcagaaa 1081 atcgccgata ttatcagcgt aattgacggc attgccttcc agaccaatat tctggctttg 1141 aacgcggcgg ttgaggctgc gcgtgcgggt gagcaagggc gcggttttgc ggtggtcgcg 1201 ggagaagtgc gtaatctggc ccagcgcagc gcccaggcgg ctcgtgaaat taaaagcctg 1261 attgaagact cggtggggaa agtggatgtt ggctctacgc tggtcgaaag cgccggggaa 1321 acaatggcgg agattgtcag cgccgtgacc cgcgtgacgg acattatggg cgaaattgct 1381 tctgcttctg atgagcagag ccgtggtatc gatcaggttg gcttagcggt tgctgagatg 1441 gaccgggtaa ctcaacagaa cgccgcgctg gtggaagagt ctgccgctgc cgccgccgcg 1501 ctggaagagc aggccagtcg cctgaccgaa gcagtggcag tgttccggat tcagcaacag 1561 cagcgtgaaa catcggctgt ggtaaaaacc gtgacgccag ctgcgccgcg taaaatggcc 1621 gtggcagata gcgaggagaa ctgggaaaca ttttaa // LOCUS BBa_I744206 1873 bp DNA linear 27-Dec-2024 DEFINITION Tet-controlled Tsr Receptor ACCESSION BBa_I744206 VERSION BBa_I744206.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..1736 /label=BBa_I744205 stem_loop 1756..1799 /label=stem_loop misc_feature 1745..1824 /label=BBa_B0010 terminator 1745..1824 /label=BBa_B0010 stem_loop 1840..1859 /label=T7 TE misc_feature 1866..1866 /label=stop polyA_site 1860..1873 /label=polya misc_feature 1833..1873 /label=BBa_B0012 terminator 1833..1873 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgttaaaac gtatcaaaat tgtgaccagc ttactgctgg 121 ttttggccgt ttttggcctt ttacaactga catcaggcgg tctgttcttt aatgccttaa 181 agaatgacaa agaaaatttc actgttttac aaaccattcg ccagcagcaa tccacgctga 241 atggcagctg ggtcgcgttg ttgcagacgc gtaacaccct caaccgcgcg ggtatccgct 301 acatgatgga tcagaataat attggtagcg gttcaaccgt tgctgagctg atggagagtg 361 ccagtatttc gctgaaacag gcggaaaaaa actgggcgga ttacgaagcg ttgccgcgtg 421 acccgcgtca gagcaccgcc gcagcggcag agatcaaacg taattacgat atttatcaca 481 atgcgctggc ggagctgatc caactgttag gtgcaggcaa aatcaacgag ttctttgatc 541 agccgaccca gggatatcag gacggtttcg agaagcagta tgtggcttac atggagcaaa 601 acgatcggct ccatgatatc gccgtcagcg ataacaatgc ctcctacagc caggcgatgt 661 ggattctggt gggcgtgatg atcgtcgtac tggcggtcat cttcgccgtc tggttcggta 721 ttaaagcctc gctggtagcg ccaatgaatc gcctgattga cagcattcgt catattgcag 781 gcggcgatct ggtgaaaccg attgaggtgg atggctctaa tgagatgggg caactggcag 841 agagtttgcg ccatatgcag ggagagctga tgcgtaccgt cggtgatgtg cgcaacgggg 901 ccaatgccat ctatagcggt gccagcgaaa tcgccaccgg caataacgat ctctcttcgc 961 gcaccgagca acaggccgct tcgctggaag agacggcagc cagcatggag caactgaccg 1021 caacggtgaa gcagaacgcc gaaaatgcgc gccaggccag ccatctggcg ttaagtgctt 1081 ctgaaacggc gcaacgcggc ggtaaagtgg tagataacgt ggtgcagact atgcgcgata 1141 tctccaccag ttcgcagaaa atcgccgata ttatcagcgt aattgacggc attgccttcc 1201 agaccaatat tctggctttg aacgcggcgg ttgaggctgc gcgtgcgggt gagcaagggc 1261 gcggttttgc ggtggtcgcg ggagaagtgc gtaatctggc ccagcgcagc gcccaggcgg 1321 ctcgtgaaat taaaagcctg attgaagact cggtggggaa agtggatgtt ggctctacgc 1381 tggtcgaaag cgccggggaa acaatggcgg agattgtcag cgccgtgacc cgcgtgacgg 1441 acattatggg cgaaattgct tctgcttctg atgagcagag ccgtggtatc gatcaggttg 1501 gcttagcggt tgctgagatg gaccgggtaa ctcaacagaa cgccgcgctg gtggaagagt 1561 ctgccgctgc cgccgccgcg ctggaagagc aggccagtcg cctgaccgaa gcagtggcag 1621 tgttccggat tcagcaacag cagcgtgaaa catcggctgt ggtaaaaacc gtgacgccag 1681 ctgcgccgcg taaaatggcc gtggcagata gcgaggagaa ctgggaaaca ttttaatact 1741 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 1801 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 1861 ttctgcgttt ata //