BBa_I744209 1 N1C2 LuxN-Tsr Chimeric Receptor B 2007-10-23T11:00:00Z 2015-08-31T04:08:03Z LuxN N-terminal domain is from ''Vibrio harveyi''. Tsr C-terminal domain is from ''E.coli''. TetR-promoter driven chimeric receptor containing the N-terminal transmembrane sensor domain (1..345) of the quorum-sensing receptor '''LuxN''' from ''Vibrio harveyi'' fused to the C-terminal signaling domain (216..551) of the ''E.coli'' MCP receptor, '''Tsr'''. The domains are linked by an NdeI site (translation = 'HM'). true false _147_ 0 1764 9 Discontinued false false Peter Nguyen annotation1954548 1 Stop Translation range1954548 1 2050 2052 annotation1954597 1 point mutation to remove natural NdeI range1954597 1 1170 1170 annotation1954550 1 Tsr signaling domain fragment 2 range1954550 1 1042 2052 annotation1954547 1 Start translation range1954547 1 1 3 annotation1954549 1 LuxN sensory domain fragment 1 range1954549 1 1 1035 BBa_I744209_sequence 1 atgtttgattttagcctagaggctatcgtctacgccaaagccattagcctcctcgcgaccgtggcagtcgtcatgatgtggctcttctattactgctatcgacttaagcaaaaaaatgaagtgatctttggtactcaccacgcagcttatattgcgtactcggtgtgtatcattgcgtggatcagcagcaatgcttatttccataccgatttactgcccgagctcggagcttcggctggcatgttcatggccaagtttgccaacctcgcttccttcttcgcatttgcttttgcctattatttctcatgccagttggcggcggagcaacgcaaaggcaaagtacaccgatggcagcaaggtatctttgtttcattaactgtgtactcactgtttatcaatttgcgtcccggtttgaccgtagagcacgtcgatattgtcggcccaagtcaattcatcatcgagtttggccctcatacctcttatttctttatcggcctcgttagctttgttgttctgactctcgtcaatcttgtcgccatgcgaacaaacagcagtaagctgactctagccaagaccaactacatgatcgccggtatcttagtgtttatgctttcgacggcagtcattcatcttggtatgacttactttatgggagatttctcattgacttggttaccaccagccttgtcgataagtgaaatgctctttgtcggctatgcactgctgacctcccgcttctacagcgtaaagtacattgcttacctcgctctcagcgtgctgctggtctgtgctattttcgttctgcctttaggcgcaatattcatcccacttacggaaagtaatcagtggcttatcgccatacctatctgcgcccttattggtattacttggcagcttttgtataaaaagaccagccgatatgcatcgttcttgatatatggcgacaagaaaacaccagtacagcaaatcttgtcacttgaagaggattttaagctttctattgatgatgcgatgcgccgtctgggtaagctgctgcatatggcctcgctggtagcgccaatgaatcgcctgattgacagcattcgtcatattgcaggcggcgatctggtgaaaccgattgaggtggatggctctaatgagatggggcaactggcagagagtttgcgccacatgcagggagagctgatgcgtaccgtcggtgatgtgcgcaacggggccaatgccatctatagcggtgccagcgaaatcgccaccggcaataacgatctctcttcgcgcaccgagcaacaggccgcttcgctggaagagacggcagccagcatggagcaactgaccgcaacggtgaagcagaacgccgaaaatgcgcgccaggccagccatctggcgttaagtgcttctgaaacggcgcaacgcggcggtaaagtggtagataacgtggtgcagactatgcgcgatatctccaccagttcgcagaaaatcgccgatattatcagcgtaattgacggcattgccttccagaccaatattctggctttgaacgcggcggttgaggctgcgcgtgcgggtgagcaagggcgcggttttgcggtggtcgcgggagaagtgcgtaatctggcccagcgcagcgcccaggcggctcgtgaaattaaaagcctgattgaagactcggtggggaaagtggatgttggctctacgctggtcgaaagcgccggggaaacaatggcggagattgtcagcgccgtgacccgcgtgacggacattatgggcgaaattgcttctgcttctgatgagcagagccgtggtatcgatcaggttggcttagcggttgctgagatggaccgggtaactcaacagaacgccgcgctggtggaagagtctgccgctgccgccgccgcgctggaagagcaggccagtcgcctgaccgaagcagtggcagtgttccggattcagcaacagcagcgtgaaacatcggctgtggtaaaaaccgtgacgccagctgcgccgcgtaaaatggccgtggcagatagcgaggagaactgggaaacattttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z