LOCUS       BBa_I744210             2132 bp    DNA     linear       25-Nov-2024
DEFINITION  TetR regulated LuxN-Tsr Chimeric Receptor B
ACCESSION   BBa_I744210
VERSION     BBa_I744210.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..83
                     /label=Start translation
     misc_feature    2130..2132
                     /label=Stop Translation
     misc_feature    81..1115
                     /label=LuxN sensory domain fragment 1
     misc_feature    1122..2132
                     /label=Tsr signaling domain fragment 2
     misc_feature    1250..1250
                     /label=point mutation to remove natural NdeI
     CDS             81..2132
                     /label=BBa_I744209
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgtttgatt ttagcctaga ggctatcgtc tacgccaaag
      121 ccattagcct cctcgcgacc gtggcagtcg tcatgatgtg gctcttctat tactgctatc
      181 gacttaagca aaaaaatgaa gtgatctttg gtactcacca cgcagcttat attgcgtact
      241 cggtgtgtat cattgcgtgg atcagcagca atgcttattt ccataccgat ttactgcccg
      301 agctcggagc ttcggctggc atgttcatgg ccaagtttgc caacctcgct tccttcttcg
      361 catttgcttt tgcctattat ttctcatgcc agttggcggc ggagcaacgc aaaggcaaag
      421 tacaccgatg gcagcaaggt atctttgttt cattaactgt gtactcactg tttatcaatt
      481 tgcgtcccgg tttgaccgta gagcacgtcg atattgtcgg cccaagtcaa ttcatcatcg
      541 agtttggccc tcatacctct tatttcttta tcggcctcgt tagctttgtt gttctgactc
      601 tcgtcaatct tgtcgccatg cgaacaaaca gcagtaagct gactctagcc aagaccaact
      661 acatgatcgc cggtatctta gtgtttatgc tttcgacggc agtcattcat cttggtatga
      721 cttactttat gggagatttc tcattgactt ggttaccacc agccttgtcg ataagtgaaa
      781 tgctctttgt cggctatgca ctgctgacct cccgcttcta cagcgtaaag tacattgctt
      841 acctcgctct cagcgtgctg ctggtctgtg ctattttcgt tctgccttta ggcgcaatat
      901 tcatcccact tacggaaagt aatcagtggc ttatcgccat acctatctgc gcccttattg
      961 gtattacttg gcagcttttg tataaaaaga ccagccgata tgcatcgttc ttgatatatg
     1021 gcgacaagaa aacaccagta cagcaaatct tgtcacttga agaggatttt aagctttcta
     1081 ttgatgatgc gatgcgccgt ctgggtaagc tgctgcatat ggcctcgctg gtagcgccaa
     1141 tgaatcgcct gattgacagc attcgtcata ttgcaggcgg cgatctggtg aaaccgattg
     1201 aggtggatgg ctctaatgag atggggcaac tggcagagag tttgcgccac atgcagggag
     1261 agctgatgcg taccgtcggt gatgtgcgca acggggccaa tgccatctat agcggtgcca
     1321 gcgaaatcgc caccggcaat aacgatctct cttcgcgcac cgagcaacag gccgcttcgc
     1381 tggaagagac ggcagccagc atggagcaac tgaccgcaac ggtgaagcag aacgccgaaa
     1441 atgcgcgcca ggccagccat ctggcgttaa gtgcttctga aacggcgcaa cgcggcggta
     1501 aagtggtaga taacgtggtg cagactatgc gcgatatctc caccagttcg cagaaaatcg
     1561 ccgatattat cagcgtaatt gacggcattg ccttccagac caatattctg gctttgaacg
     1621 cggcggttga ggctgcgcgt gcgggtgagc aagggcgcgg ttttgcggtg gtcgcgggag
     1681 aagtgcgtaa tctggcccag cgcagcgccc aggcggctcg tgaaattaaa agcctgattg
     1741 aagactcggt ggggaaagtg gatgttggct ctacgctggt cgaaagcgcc ggggaaacaa
     1801 tggcggagat tgtcagcgcc gtgacccgcg tgacggacat tatgggcgaa attgcttctg
     1861 cttctgatga gcagagccgt ggtatcgatc aggttggctt agcggttgct gagatggacc
     1921 gggtaactca acagaacgcc gcgctggtgg aagagtctgc cgctgccgcc gccgcgctgg
     1981 aagagcaggc cagtcgcctg accgaagcag tggcagtgtt ccggattcag caacagcagc
     2041 gtgaaacatc ggctgtggta aaaaccgtga cgccagctgc gccgcgtaaa atggccgtgg
     2101 cagatagcga ggagaactgg gaaacatttt aa
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