BBa_I744211 1 N2C1 LuxN-Tsr Chimeric Receptor C 2007-10-23T11:00:00Z 2015-08-31T04:08:03Z LuxN N-terminal domain is from ''Vibrio harveyi''. Tsr C-terminal domain is from ''E.coli''. TetR-promoter driven chimeric receptor containing the N-terminal transmembrane sensor domain (1..442) of the quorum-sensing receptor '''LuxN''' from ''Vibrio harveyi'' fused to the C-terminal signaling domain (260..551) of the ''E.coli'' MCP receptor, '''Tsr'''. The domains are linked by an NdeI site (translation = 'HM'). true false _147_ 0 1764 9 Discontinued false false Peter Nguyen annotation1954563 1 Start translation range1954563 1 1 3 annotation1954566 1 Tsr signaling domain fragment 1 range1954566 1 1333 2211 annotation1954565 1 LuxN sensory domain fragment 2 range1954565 1 1 1326 annotation1954564 1 Stop translation range1954564 1 2209 2211 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986785 1 -35 range1986785 1 20 25 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I744212 1 BBa_I744212 TetR regulated LuxN-Tsr Chimeric Receptor C 2007-10-23T11:00:00Z 2015-08-31T04:08:03Z LuxN N-terminal domain is from ''Vibrio harveyi''. Tsr C-terminal domain is from ''E.coli''. TetR-promoter driven chimeric receptor containing the N-terminal transmembrane sensor domain (1..442) of the quorum-sensing receptor '''LuxN''' from ''Vibrio harveyi'' fused to the C-terminal signaling domain (260..551) of the ''E.coli'' MCP receptor, '''Tsr'''. The domains are linked by an NdeI site (translation = 'HM'). false false _147_ 0 1764 9 It's complicated false false Peter Nguyen component1954568 1 BBa_R0040 component1954578 1 BBa_I744211 component1954573 1 BBa_B0034 annotation1954578 1 BBa_I744211 range1954578 1 81 2291 annotation1954573 1 BBa_B0034 range1954573 1 63 74 annotation1954568 1 BBa_R0040 range1954568 1 1 54 BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_I744211_sequence 1 atgtttgattttagcctagaggctatcgtctacgccaaagccattagcctcctcgcgaccgtggcagtcgtcatgatgtggctcttctattactgctatcgacttaagcaaaaaaatgaagtgatctttggtactcaccacgcagcttatattgcgtactcggtgtgtatcattgcgtggatcagcagcaatgcttatttccataccgatttactgcccgagctcggagcttcggctggcatgttcatggccaagtttgccaacctcgcttccttcttcgcatttgcttttgcctattatttctcatgccagttggcggcggagcaacgcaaaggcaaagtacaccgatggcagcaaggtatctttgtttcattaactgtgtactcactgtttatcaatttgcgtcccggtttgaccgtagagcacgtcgatattgtcggcccaagtcaattcatcatcgagtttggccctcatacctcttatttctttatcggcctcgttagctttgttgttctgactctcgtcaatcttgtcgccatgcgaacaaacagcagtaagctgactctagccaagaccaactacatgatcgccggtatcttagtgtttatgctttcgacggcagtcattcatcttggtatgacttactttatgggagatttctcattgacttggttaccaccagccttgtcgataagtgaaatgctctttgtcggctatgcactgctgacctcccgcttctacagcgtaaagtacattgcttacctcgctctcagcgtgctgctggtctgtgctattttcgttctgcctttaggcgcaatattcatcccacttacggaaagtaatcagtggcttatcgccatacctatctgcgcccttattggtattacttggcagcttttgtataaaaagaccagccgatatgcatcgttcttgatatatggcgacaagaaaacaccagtacagcaaatcttgtcacttgaagaggattttaagctttctattgatgatgcgatgcgccgtctgggtaagctgctgcaaattccaaatgacaaactacgcctcgtaaccagtaactacaacgaaaccttttacgaagagtacctttcatcaaaccgctcggtgttggtgtttgatgaactctctgaagagctcgaatacaaggtatcggcaaagcgttcaatgaaggcgctgtatgacaaaatgagctcaaacaacaccgctttggttatgccgctgtttgggcaaggtaaatcggttacgcacttattgatttctccccacaagagcaacaaccaaatgttctcgaacgaggaaatctcagcagttcatatgcagggagagctgatgcgtaccgtcggtgatgtgcgcaacggggccaatgccatctatagcggtgccagcgaaatcgccaccggcaataacgatctctcttcgcgcaccgagcaacaggccgcttcgctggaagagacggcagccagcatggagcaactgaccgcaacggtgaagcagaacgccgaaaatgcgcgccaggccagccatctggcgttaagtgcttctgaaacggcgcaacgcggcggtaaagtggtagataacgtggtgcagactatgcgcgatatctccaccagttcgcagaaaatcgccgatattatcagcgtaattgacggcattgccttccagaccaatattctggctttgaacgcggcggttgaggctgcgcgtgcgggtgagcaagggcgcggttttgcggtggtcgcgggagaagtgcgtaatctggcccagcgcagcgcccaggcggctcgtgaaattaaaagcctgattgaagactcggtggggaaagtggatgttggctctacgctggtcgaaagcgccggggaaacaatggcggagattgtcagcgccgtgacccgcgtgacggacattatgggcgaaattgcttctgcttctgatgagcagagccgtggtatcgatcaggttggcttagcggttgctgagatggaccgggtaactcaacagaacgccgcgctggtggaagagtctgccgctgccgccgccgcgctggaagagcaggccagtcgcctgaccgaagcagtggcagtgttccggattcagcaacagcagcgtgaaacatcggctgtggtaaaaaccgtgacgccagctgcgccgcgtaaaatggccgtggcagatagcgaggagaactgggaaacattttaa BBa_I744212_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgtttgattttagcctagaggctatcgtctacgccaaagccattagcctcctcgcgaccgtggcagtcgtcatgatgtggctcttctattactgctatcgacttaagcaaaaaaatgaagtgatctttggtactcaccacgcagcttatattgcgtactcggtgtgtatcattgcgtggatcagcagcaatgcttatttccataccgatttactgcccgagctcggagcttcggctggcatgttcatggccaagtttgccaacctcgcttccttcttcgcatttgcttttgcctattatttctcatgccagttggcggcggagcaacgcaaaggcaaagtacaccgatggcagcaaggtatctttgtttcattaactgtgtactcactgtttatcaatttgcgtcccggtttgaccgtagagcacgtcgatattgtcggcccaagtcaattcatcatcgagtttggccctcatacctcttatttctttatcggcctcgttagctttgttgttctgactctcgtcaatcttgtcgccatgcgaacaaacagcagtaagctgactctagccaagaccaactacatgatcgccggtatcttagtgtttatgctttcgacggcagtcattcatcttggtatgacttactttatgggagatttctcattgacttggttaccaccagccttgtcgataagtgaaatgctctttgtcggctatgcactgctgacctcccgcttctacagcgtaaagtacattgcttacctcgctctcagcgtgctgctggtctgtgctattttcgttctgcctttaggcgcaatattcatcccacttacggaaagtaatcagtggcttatcgccatacctatctgcgcccttattggtattacttggcagcttttgtataaaaagaccagccgatatgcatcgttcttgatatatggcgacaagaaaacaccagtacagcaaatcttgtcacttgaagaggattttaagctttctattgatgatgcgatgcgccgtctgggtaagctgctgcaaattccaaatgacaaactacgcctcgtaaccagtaactacaacgaaaccttttacgaagagtacctttcatcaaaccgctcggtgttggtgtttgatgaactctctgaagagctcgaatacaaggtatcggcaaagcgttcaatgaaggcgctgtatgacaaaatgagctcaaacaacaccgctttggttatgccgctgtttgggcaaggtaaatcggttacgcacttattgatttctccccacaagagcaacaaccaaatgttctcgaacgaggaaatctcagcagttcatatgcagggagagctgatgcgtaccgtcggtgatgtgcgcaacggggccaatgccatctatagcggtgccagcgaaatcgccaccggcaataacgatctctcttcgcgcaccgagcaacaggccgcttcgctggaagagacggcagccagcatggagcaactgaccgcaacggtgaagcagaacgccgaaaatgcgcgccaggccagccatctggcgttaagtgcttctgaaacggcgcaacgcggcggtaaagtggtagataacgtggtgcagactatgcgcgatatctccaccagttcgcagaaaatcgccgatattatcagcgtaattgacggcattgccttccagaccaatattctggctttgaacgcggcggttgaggctgcgcgtgcgggtgagcaagggcgcggttttgcggtggtcgcgggagaagtgcgtaatctggcccagcgcagcgcccaggcggctcgtgaaattaaaagcctgattgaagactcggtggggaaagtggatgttggctctacgctggtcgaaagcgccggggaaacaatggcggagattgtcagcgccgtgacccgcgtgacggacattatgggcgaaattgcttctgcttctgatgagcagagccgtggtatcgatcaggttggcttagcggttgctgagatggaccgggtaactcaacagaacgccgcgctggtggaagagtctgccgctgccgccgccgcgctggaagagcaggccagtcgcctgaccgaagcagtggcagtgttccggattcagcaacagcagcgtgaaacatcggctgtggtaaaaaccgtgacgccagctgcgccgcgtaaaatggccgtggcagatagcgaggagaactgggaaacattttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z