LOCUS       BBa_I744214             2423 bp    DNA     linear       16-Feb-2025
DEFINITION  TetR regulated LuxN-Tsr Chimeric Receptor D
ACCESSION   BBa_I744214
VERSION     BBa_I744214.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..83
                     /label=Start translation
     misc_feature    2421..2423
                     /label=Stop translation
     misc_feature    81..1406
                     /label=LuxN sensory domain fragment 2
     misc_feature    1413..2423
                     /label=Tsr signaling domain fragment 2
     misc_feature    1541..1541
                     /label=point mutation to remove natural NdeI
     CDS             81..2423
                     /label=BBa_I744213
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgtttgatt ttagcctaga ggctatcgtc tacgccaaag
      121 ccattagcct cctcgcgacc gtggcagtcg tcatgatgtg gctcttctat tactgctatc
      181 gacttaagca aaaaaatgaa gtgatctttg gtactcacca cgcagcttat attgcgtact
      241 cggtgtgtat cattgcgtgg atcagcagca atgcttattt ccataccgat ttactgcccg
      301 agctcggagc ttcggctggc atgttcatgg ccaagtttgc caacctcgct tccttcttcg
      361 catttgcttt tgcctattat ttctcatgcc agttggcggc ggagcaacgc aaaggcaaag
      421 tacaccgatg gcagcaaggt atctttgttt cattaactgt gtactcactg tttatcaatt
      481 tgcgtcccgg tttgaccgta gagcacgtcg atattgtcgg cccaagtcaa ttcatcatcg
      541 agtttggccc tcatacctct tatttcttta tcggcctcgt tagctttgtt gttctgactc
      601 tcgtcaatct tgtcgccatg cgaacaaaca gcagtaagct gactctagcc aagaccaact
      661 acatgatcgc cggtatctta gtgtttatgc tttcgacggc agtcattcat cttggtatga
      721 cttactttat gggagatttc tcattgactt ggttaccacc agccttgtcg ataagtgaaa
      781 tgctctttgt cggctatgca ctgctgacct cccgcttcta cagcgtaaag tacattgctt
      841 acctcgctct cagcgtgctg ctggtctgtg ctattttcgt tctgccttta ggcgcaatat
      901 tcatcccact tacggaaagt aatcagtggc ttatcgccat acctatctgc gcccttattg
      961 gtattacttg gcagcttttg tataaaaaga ccagccgata tgcatcgttc ttgatatatg
     1021 gcgacaagaa aacaccagta cagcaaatct tgtcacttga agaggatttt aagctttcta
     1081 ttgatgatgc gatgcgccgt ctgggtaagc tgctgcaaat tccaaatgac aaactacgcc
     1141 tcgtaaccag taactacaac gaaacctttt acgaagagta cctttcatca aaccgctcgg
     1201 tgttggtgtt tgatgaactc tctgaagagc tcgaatacaa ggtatcggca aagcgttcaa
     1261 tgaaggcgct gtatgacaaa atgagctcaa acaacaccgc tttggttatg ccgctgtttg
     1321 ggcaaggtaa atcggttacg cacttattga tttctcccca caagagcaac aaccaaatgt
     1381 tctcgaacga ggaaatctca gcagttcata tggcctcgct ggtagcgcca atgaatcgcc
     1441 tgattgacag cattcgtcat attgcaggcg gcgatctggt gaaaccgatt gaggtggatg
     1501 gctctaatga gatggggcaa ctggcagaga gtttgcgcca catgcaggga gagctgatgc
     1561 gtaccgtcgg tgatgtgcgc aacggggcca atgccatcta tagcggtgcc agcgaaatcg
     1621 ccaccggcaa taacgatctc tcttcgcgca ccgagcaaca ggccgcttcg ctggaagaga
     1681 cggcagccag catggagcaa ctgaccgcaa cggtgaagca gaacgccgaa aatgcgcgcc
     1741 aggccagcca tctggcgtta agtgcttctg aaacggcgca acgcggcggt aaagtggtag
     1801 ataacgtggt gcagactatg cgcgatatct ccaccagttc gcagaaaatc gccgatatta
     1861 tcagcgtaat tgacggcatt gccttccaga ccaatattct ggctttgaac gcggcggttg
     1921 aggctgcgcg tgcgggtgag caagggcgcg gttttgcggt ggtcgcggga gaagtgcgta
     1981 atctggccca gcgcagcgcc caggcggctc gtgaaattaa aagcctgatt gaagactcgg
     2041 tggggaaagt ggatgttggc tctacgctgg tcgaaagcgc cggggaaaca atggcggaga
     2101 ttgtcagcgc cgtgacccgc gtgacggaca ttatgggcga aattgcttct gcttctgatg
     2161 agcagagccg tggtatcgat caggttggct tagcggttgc tgagatggac cgggtaactc
     2221 aacagaacgc cgcgctggtg gaagagtctg ccgctgccgc cgccgcgctg gaagagcagg
     2281 ccagtcgcct gaccgaagca gtggcagtgt tccggattca gcaacagcag cgtgaaacat
     2341 cggctgtggt aaaaaccgtg acgccagctg cgccgcgtaa aatggccgtg gcagatagcg
     2401 aggagaactg ggaaacattt taa
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