BBa_I744214 1 BBa_I744214 TetR regulated LuxN-Tsr Chimeric Receptor D 2007-10-23T11:00:00Z 2015-08-31T04:08:03Z LuxN N-terminal domain is from Vibrio harveyi. Tsr C-terminal domain is from E.coli. TetR-promoter driven chimeric receptor containing the N-terminal transmembrane sensor domain (1..442) of the quorum-sensing receptor LuxN from Vibrio harveyi fused to the C-terminal signaling domain (216..551) of the E.coli MCP receptor, Tsr. The domains are linked by an NdeI site (translation = 'HM'). false false _147_ 0 1764 9 It's complicated false The internal NdeI (CATATG) site of the Tsr C-terminal fragment 2 occuring at 772bp of the original protein receptor gene was mutated to (CACATG). This allowed the fusion of the LuxN and Tsr fragments by cloning in an NdeI site upstream at 646bp of Tsr, to test the effects of different fragment lengths. false Peter Nguyen component1954590 1 BBa_B0034 component1954596 1 BBa_I744213 component1954585 1 BBa_R0040 annotation1954590 1 BBa_B0034 range1954590 1 63 74 annotation1954596 1 BBa_I744213 range1954596 1 81 2423 annotation1954585 1 BBa_R0040 range1954585 1 1 54 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I744213 1 N2C2 LuxN-Tsr Chimeric Receptor D 2007-10-23T11:00:00Z 2015-08-31T04:08:03Z LuxN N-terminal domain is from ''Vibrio harveyi''. Tsr C-terminal domain is from ''E.coli''. TetR-promoter driven chimeric receptor containing the N-terminal transmembrane sensor domain (1..442) of the quorum-sensing receptor '''LuxN''' from ''Vibrio harveyi'' fused to the C-terminal signaling domain (216..551) of the ''E.coli'' MCP receptor, '''Tsr'''. The domains are linked by an NdeI site (translation = 'HM'). true false _147_ 0 1764 9 Discontinued false The internal NdeI (CATATG) site of the Tsr C-terminal fragment 2 occuring at 772bp of the original protein receptor gene was mutated to (CACATG). This allowed the fusion of the LuxN and Tsr fragments by cloning in an NdeI site upstream at 646bp of Tsr, to test the effects of different fragment lengths. false Peter Nguyen annotation1954579 1 Start translation range1954579 1 1 3 annotation1954580 1 Stop translation range1954580 1 2341 2343 annotation1954583 1 point mutation to remove natural NdeI range1954583 1 1461 1461 annotation1954582 1 Tsr signaling domain fragment 2 range1954582 1 1333 2343 annotation1954581 1 LuxN sensory domain fragment 2 range1954581 1 1 1326 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986787 1 -10 range1986787 1 43 48 annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986785 1 -35 range1986785 1 20 25 annotation1986783 1 TetR 1 range1986783 1 1 19 BBa_I744213_sequence 1 atgtttgattttagcctagaggctatcgtctacgccaaagccattagcctcctcgcgaccgtggcagtcgtcatgatgtggctcttctattactgctatcgacttaagcaaaaaaatgaagtgatctttggtactcaccacgcagcttatattgcgtactcggtgtgtatcattgcgtggatcagcagcaatgcttatttccataccgatttactgcccgagctcggagcttcggctggcatgttcatggccaagtttgccaacctcgcttccttcttcgcatttgcttttgcctattatttctcatgccagttggcggcggagcaacgcaaaggcaaagtacaccgatggcagcaaggtatctttgtttcattaactgtgtactcactgtttatcaatttgcgtcccggtttgaccgtagagcacgtcgatattgtcggcccaagtcaattcatcatcgagtttggccctcatacctcttatttctttatcggcctcgttagctttgttgttctgactctcgtcaatcttgtcgccatgcgaacaaacagcagtaagctgactctagccaagaccaactacatgatcgccggtatcttagtgtttatgctttcgacggcagtcattcatcttggtatgacttactttatgggagatttctcattgacttggttaccaccagccttgtcgataagtgaaatgctctttgtcggctatgcactgctgacctcccgcttctacagcgtaaagtacattgcttacctcgctctcagcgtgctgctggtctgtgctattttcgttctgcctttaggcgcaatattcatcccacttacggaaagtaatcagtggcttatcgccatacctatctgcgcccttattggtattacttggcagcttttgtataaaaagaccagccgatatgcatcgttcttgatatatggcgacaagaaaacaccagtacagcaaatcttgtcacttgaagaggattttaagctttctattgatgatgcgatgcgccgtctgggtaagctgctgcaaattccaaatgacaaactacgcctcgtaaccagtaactacaacgaaaccttttacgaagagtacctttcatcaaaccgctcggtgttggtgtttgatgaactctctgaagagctcgaatacaaggtatcggcaaagcgttcaatgaaggcgctgtatgacaaaatgagctcaaacaacaccgctttggttatgccgctgtttgggcaaggtaaatcggttacgcacttattgatttctccccacaagagcaacaaccaaatgttctcgaacgaggaaatctcagcagttcatatggcctcgctggtagcgccaatgaatcgcctgattgacagcattcgtcatattgcaggcggcgatctggtgaaaccgattgaggtggatggctctaatgagatggggcaactggcagagagtttgcgccacatgcagggagagctgatgcgtaccgtcggtgatgtgcgcaacggggccaatgccatctatagcggtgccagcgaaatcgccaccggcaataacgatctctcttcgcgcaccgagcaacaggccgcttcgctggaagagacggcagccagcatggagcaactgaccgcaacggtgaagcagaacgccgaaaatgcgcgccaggccagccatctggcgttaagtgcttctgaaacggcgcaacgcggcggtaaagtggtagataacgtggtgcagactatgcgcgatatctccaccagttcgcagaaaatcgccgatattatcagcgtaattgacggcattgccttccagaccaatattctggctttgaacgcggcggttgaggctgcgcgtgcgggtgagcaagggcgcggttttgcggtggtcgcgggagaagtgcgtaatctggcccagcgcagcgcccaggcggctcgtgaaattaaaagcctgattgaagactcggtggggaaagtggatgttggctctacgctggtcgaaagcgccggggaaacaatggcggagattgtcagcgccgtgacccgcgtgacggacattatgggcgaaattgcttctgcttctgatgagcagagccgtggtatcgatcaggttggcttagcggttgctgagatggaccgggtaactcaacagaacgccgcgctggtggaagagtctgccgctgccgccgccgcgctggaagagcaggccagtcgcctgaccgaagcagtggcagtgttccggattcagcaacagcagcgtgaaacatcggctgtggtaaaaaccgtgacgccagctgcgccgcgtaaaatggccgtggcagatagcgaggagaactgggaaacattttaa BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_I744214_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgtttgattttagcctagaggctatcgtctacgccaaagccattagcctcctcgcgaccgtggcagtcgtcatgatgtggctcttctattactgctatcgacttaagcaaaaaaatgaagtgatctttggtactcaccacgcagcttatattgcgtactcggtgtgtatcattgcgtggatcagcagcaatgcttatttccataccgatttactgcccgagctcggagcttcggctggcatgttcatggccaagtttgccaacctcgcttccttcttcgcatttgcttttgcctattatttctcatgccagttggcggcggagcaacgcaaaggcaaagtacaccgatggcagcaaggtatctttgtttcattaactgtgtactcactgtttatcaatttgcgtcccggtttgaccgtagagcacgtcgatattgtcggcccaagtcaattcatcatcgagtttggccctcatacctcttatttctttatcggcctcgttagctttgttgttctgactctcgtcaatcttgtcgccatgcgaacaaacagcagtaagctgactctagccaagaccaactacatgatcgccggtatcttagtgtttatgctttcgacggcagtcattcatcttggtatgacttactttatgggagatttctcattgacttggttaccaccagccttgtcgataagtgaaatgctctttgtcggctatgcactgctgacctcccgcttctacagcgtaaagtacattgcttacctcgctctcagcgtgctgctggtctgtgctattttcgttctgcctttaggcgcaatattcatcccacttacggaaagtaatcagtggcttatcgccatacctatctgcgcccttattggtattacttggcagcttttgtataaaaagaccagccgatatgcatcgttcttgatatatggcgacaagaaaacaccagtacagcaaatcttgtcacttgaagaggattttaagctttctattgatgatgcgatgcgccgtctgggtaagctgctgcaaattccaaatgacaaactacgcctcgtaaccagtaactacaacgaaaccttttacgaagagtacctttcatcaaaccgctcggtgttggtgtttgatgaactctctgaagagctcgaatacaaggtatcggcaaagcgttcaatgaaggcgctgtatgacaaaatgagctcaaacaacaccgctttggttatgccgctgtttgggcaaggtaaatcggttacgcacttattgatttctccccacaagagcaacaaccaaatgttctcgaacgaggaaatctcagcagttcatatggcctcgctggtagcgccaatgaatcgcctgattgacagcattcgtcatattgcaggcggcgatctggtgaaaccgattgaggtggatggctctaatgagatggggcaactggcagagagtttgcgccacatgcagggagagctgatgcgtaccgtcggtgatgtgcgcaacggggccaatgccatctatagcggtgccagcgaaatcgccaccggcaataacgatctctcttcgcgcaccgagcaacaggccgcttcgctggaagagacggcagccagcatggagcaactgaccgcaacggtgaagcagaacgccgaaaatgcgcgccaggccagccatctggcgttaagtgcttctgaaacggcgcaacgcggcggtaaagtggtagataacgtggtgcagactatgcgcgatatctccaccagttcgcagaaaatcgccgatattatcagcgtaattgacggcattgccttccagaccaatattctggctttgaacgcggcggttgaggctgcgcgtgcgggtgagcaagggcgcggttttgcggtggtcgcgggagaagtgcgtaatctggcccagcgcagcgcccaggcggctcgtgaaattaaaagcctgattgaagactcggtggggaaagtggatgttggctctacgctggtcgaaagcgccggggaaacaatggcggagattgtcagcgccgtgacccgcgtgacggacattatgggcgaaattgcttctgcttctgatgagcagagccgtggtatcgatcaggttggcttagcggttgctgagatggaccgggtaactcaacagaacgccgcgctggtggaagagtctgccgctgccgccgccgcgctggaagagcaggccagtcgcctgaccgaagcagtggcagtgttccggattcagcaacagcagcgtgaaacatcggctgtggtaaaaaccgtgacgccagctgcgccgcgtaaaatggccgtggcagatagcgaggagaactgggaaacattttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z