LOCUS BBa_I745000 9519 bp DNA linear 25-Nov-2024 DEFINITION Backbone TRE plasmid ACCESSION BBa_I745000 VERSION BBa_I745000.1 FEATURES Location/Qualifiers ORIGIN 1 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 61 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 121 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 181 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 241 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 301 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 361 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 421 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 481 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 541 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 601 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 661 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 721 aacaaattgg ctgtggtata tagaaattat tcataatgat agtaggaggc ttggtaggtt 781 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat 841 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag 901 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tcggcactgc 961 gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag aaaagggggg 1021 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact 1081 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc 1141 agagatccag tttggttaat taacgagttt actccctatc agtgatagag aacgtatgtc 1201 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag 1261 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct 1321 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg 1381 agtttactcc ctatcagtga tagagaacgt atgtcgaggt aggcgtgtac ggtgggaggc 1441 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tgagttagaa ttcggccatt 1501 acggccgcta gcgttaacgt cgacggccgc ctcggccatc gataaggatc cggaatgccc 1561 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc 1621 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa 1681 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat 1741 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac 1801 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg 1861 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt 1921 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg 1981 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac 2041 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga 2101 cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatggtg agcaagggcg 2161 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc 2221 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga 2281 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga 2341 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca 2401 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca 2461 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc 2521 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact 2581 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact 2641 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga 2701 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt 2761 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga 2821 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaagcggcc gcaatcaacc 2881 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 2941 gctatgtgga tacgctgctt taatgccttt gtatcatgct attacttccc gtacggcttt 3001 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 3061 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 3121 cattgccacc acctatcaac tcctttccgg gactttcgct ttccccctcc ctattgccac 3181 ggcggaactc attgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 3241 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 3301 tgccaactgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 3361 ggaccttcct tcccgcggcc tgctgccggt tctgcggcct cttccgcgtc ttcgccttcg 3421 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgcctgca ggtttgtcga 3481 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt 3541 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct 3601 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg 3661 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg 3721 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 3781 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 3841 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg 3901 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 3961 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 4021 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 4081 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 4141 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca 4201 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 4261 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 4321 gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag 4381 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 4441 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 4501 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 4561 agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 4621 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 4681 ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 4741 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 4801 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 4861 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 4921 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 4981 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca 5041 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc 5101 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac 5161 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac 5221 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg 5281 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac 5341 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag 5401 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga 5461 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 5521 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 5581 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 5641 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 5701 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 5761 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 5821 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 5881 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 5941 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 6001 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 6061 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 6121 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 6181 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 6241 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 6301 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc 6361 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 6421 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 6481 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 6541 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 6601 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 6661 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 6721 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 6781 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 6841 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 6901 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 6961 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 7021 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 7081 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 7141 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 7201 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 7261 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 7321 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 7381 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 7441 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 7501 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 7561 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 7621 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 7681 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 7741 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 7801 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 7861 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac 7921 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg 7981 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga 8041 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta 8101 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat 8161 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg 8221 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc 8281 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt 8341 gacgtcaatg ggtggactat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc 8401 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 8461 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 8521 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 8581 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 8641 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 8701 ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg 8761 cttactggct tatcgaaatt aatacgactc actataggga gacccaagct ggtttaaact 8821 taagcttggt accgagctca ctagtccagt gtggtggcag atatccagca cagtggcggc 8881 cgctcgagtc tagagggccc gttttgcctg tactgggtct ctctggttag accagatctg 8941 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc 9001 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct 9061 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa 9121 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg 9181 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag 9241 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg 9301 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg 9361 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc 9421 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga 9481 tcattatata atacagtagc aaccctctat tgtgtgcat //