LOCUS       BBa_I746008             2114 bp    DNA     linear       17-May-2024
DEFINITION  I0500 (Inducible pBad/araC) + I746001 (AIP generator - agrB + agrD 
            with RBSes + Terminator)
ACCESSION   BBa_I746008
VERSION     BBa_I746008.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     RBS             1219..1230
                     /label=B0034-sequence RBS
     CDS             1237..1809
                     /label=agrB coding sequence
     RBS             1816..1827
                     /label=B0034-sequence RBS
     misc_feature    1810..1815
                     /label=SaII site
     CDS             1834..1977
                     /label=agrD coding sequence
     misc_feature    1219..1977
                     /label=BBa_I746000
     stem_loop       1997..2040
                     /label=stem_loop
     misc_feature    1986..2065
                     /label=BBa_B0010
     terminator      1986..2065
                     /label=BBa_B0010
     stem_loop       2081..2100
                     /label=T7 TE
     misc_feature    2107..2107
                     /label=stop
     polyA_site      2101..2114
                     /label=polya
     misc_feature    2074..2114
                     /label=BBa_B0012
     terminator      2074..2114
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga actattttga caacaagatc
     1261 gaccaatttg ctacttacct gcaaaaacgc aacaacctgg accacatcca gtttctgcaa
     1321 gttcgtctgg gcatgcaggt tctggctaaa aacatcggca aactgatcgt tatgtacacc
     1381 atcgcctata ttctgaacat cttcctgttt accctgatca ccaacctgac cttctatctg
     1441 attcgccgtc atgcccacgg cgctcatgca ccttcttcct tttggtgtta tgtagagagc
     1501 attattctgt ttatcctgct gccgctggta atcgttaact tccacatcaa tttcctgatt
     1561 atgatcatcc tgactgtgat ttccctgggt gtaatctctg tttacgctcc ggctgccact
     1621 aagaaaaagc cgattccggt gcgcctgatc aaacgtaaaa agtactacgc tatcatcgtt
     1681 tccctgaccc tgttcattat caccctgatt atcaaagaac cgtttgcaca gttcatccag
     1741 ctgggtatta tcatcgaagc gatcactctg ctgccgatct ttttcatcaa agaggatctg
     1801 aaataataag tcgacaaaga ggagaaatac tagatgaata ctctgttcaa tctgtttttc
     1861 gatttcatta ctggcatcct gaagaacatc ggtaacatcg cagcgtacag cacctgcgac
     1921 ttcatcatgg atgaagtaga ggtcccgaaa gagctgaccc agctgcatga ataataatac
     1981 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     2041 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     2101 tttctgcgtt tata
//