LOCUS       BBa_I746009             3310 bp    DNA     linear       25-Nov-2024
DEFINITION  AIP generator + outer membrane permeability
ACCESSION   BBa_I746009
VERSION     BBa_I746009.1  
FEATURES             Location/Qualifiers
     RBS             1..12
                     /label=B0034-sequence RBS
     CDS             19..591
                     /label=agrB coding sequence
     RBS             598..609
                     /label=B0034-sequence RBS
     misc_feature    592..597
                     /label=SaII site
     CDS             616..759
                     /label=agrD coding sequence
     misc_feature    1..759
                     /label=BBa_I746000
     stem_loop       779..822
                     /label=stem_loop
     misc_feature    768..847
                     /label=BBa_B0010
     terminator      768..847
                     /label=BBa_B0010
     stem_loop       863..882
                     /label=T7 TE
     misc_feature    889..889
                     /label=stop
     polyA_site      883..896
                     /label=polya
     misc_feature    856..896
                     /label=BBa_B0012
     terminator      856..896
                     /label=BBa_B0012
     promoter        905..939
                     /label=BBa_J23111
     misc_feature    952..955
                     /label=conserved
     RBS             948..959
                     /label=BBa_B0034
     CDS             966..3173
                     /label=BBa_I746200
     stem_loop       3193..3236
                     /label=stem_loop
     misc_feature    3182..3261
                     /label=BBa_B0010
     terminator      3182..3261
                     /label=BBa_B0010
     stem_loop       3277..3296
                     /label=T7 TE
     misc_feature    3303..3303
                     /label=stop
     polyA_site      3297..3310
                     /label=polya
     misc_feature    3270..3310
                     /label=BBa_B0012
     terminator      3270..3310
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gaactatttt gacaacaaga tcgaccaatt tgctacttac
       61 ctgcaaaaac gcaacaacct ggaccacatc cagtttctgc aagttcgtct gggcatgcag
      121 gttctggcta aaaacatcgg caaactgatc gttatgtaca ccatcgccta tattctgaac
      181 atcttcctgt ttaccctgat caccaacctg accttctatc tgattcgccg tcatgcccac
      241 ggcgctcatg caccttcttc cttttggtgt tatgtagaga gcattattct gtttatcctg
      301 ctgccgctgg taatcgttaa cttccacatc aatttcctga ttatgatcat cctgactgtg
      361 atttccctgg gtgtaatctc tgtttacgct ccggctgcca ctaagaaaaa gccgattccg
      421 gtgcgcctga tcaaacgtaa aaagtactac gctatcatcg tttccctgac cctgttcatt
      481 atcaccctga ttatcaaaga accgtttgca cagttcatcc agctgggtat tatcatcgaa
      541 gcgatcactc tgctgccgat ctttttcatc aaagaggatc tgaaataata agtcgacaaa
      601 gaggagaaat actagatgaa tactctgttc aatctgtttt tcgatttcat tactggcatc
      661 ctgaagaaca tcggtaacat cgcagcgtac agcacctgcg acttcatcat ggatgaagta
      721 gaggtcccga aagagctgac ccagctgcat gaataataat actagagcca ggcatcaaat
      781 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      841 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact
      901 agagttgacg gctagctcag tcctaggtat agtgctagct actagagaaa gaggagaaat
      961 actagatgaa caagaagatt cattccctgg ccttgttggt caatctgggg atttatgggg
     1021 tagcgcaggc acaagagccg accgatactc ctgtttcaca tgacgatact attgtcgtta
     1081 ccgccgccga gcagaactta caggcgcctg gcgtttcgac catcaccgca gatgaaatcc
     1141 gcaaaaaccc ggttgcccgc gatgtgtcga agatcatccg taccatgcca ggcgttaacc
     1201 tgaccggtaa ctccaccagt ggtcagcgtg ggaataaccg acagattgat attcgcggta
     1261 tgggtccgga aaacacgctg attttgattg acggcaagcc ggtaagcagc cgtaactcgg
     1321 tgcgtcaggg ctggcgtggc gagcgcgata cccgtggtga tacttcctgg gtgccacctg
     1381 aaatgattga acgtattgaa gttctgcgtg gtccggcagc tgcgcgttat ggcaacggcg
     1441 cggcgggcgg cgtggttaac atcattacca aaaaaggcag cggcgagtgg cacggctcct
     1501 gggacgcata tttcaatgcg ccagaacata aagaggaagg tgccaccaaa cgcactaact
     1561 ttagcctgac cggtccgctg ggcgacgagt tcagcttccg tttgtatggc aacctcgaca
     1621 aaacccaggc tgacgcgtgg gatatcaacc agggccatca gtccgcgcgt gccggaacgt
     1681 atgccacgac gttaccagcc gggcgcgaag gggtaatcaa caaagatatt aatggcgtgg
     1741 tgcgctggga tttcgcgcca ttgcaatcgc tggaactgga agcaggttac agccgccagg
     1801 gtaacctgta tgcgggcgac acccagaata ccaactccga ttcctatacc cgctcgaaat
     1861 atggcgatga aaccaaccgt ctgtatcgcc agaactacgc gctgacctgg aacggtggct
     1921 gggataacgg cgtgaccacc agcaactggg tgcagtacga acacacccgt aactcgcgta
     1981 ttccggaagg tctggcgggc ggtaccgaag ggaaatttaa cgaaaaagcg acacaggatt
     2041 tcgtcgatat cgatcttgat gacgtgatgc tgcacagcga agttaacctg ccgattgatt
     2101 tcctcgttaa ccagacgctg acgctgggta cggagtggaa tcagcaacgg atgaaggact
     2161 taagttccaa cacccaggca ctgaccggaa cgaataccgg tggcgctatt gatggcgtga
     2221 gtaccaccga ccgtagcccg tattcaaaag cagaaatttt ctcgctgttt gccgaaaaca
     2281 acatggagct gactgacagc accatcgtaa cgccggggct gcgtttcgat catcacagta
     2341 ttgtcggcaa taactggagc ccggcgctga acatatcgca aggtttaggc gatgacttca
     2401 cgctgaaaat gggcatcgcc cgtgcttata aagcgccgag cctgtaccag actaacccga
     2461 actacattct ctacagtaaa ggtcagggtt gctatgccag cgcgggcggc tgctatctgc
     2521 aaggtaacga tgacctgaaa gcagaaacca gcatcaacaa agagattggt ctggagttca
     2581 aacgcgacgg gtggctggcg ggcgtcacct ggttccgtaa cgattatcgc aataagattg
     2641 aagcgggcta tgtggctgta gggcaaaatt gtgaaacagc ggtggttgaa ggtctggaag
     2701 gatcgttaaa cgtaccggtt agcgaaacgg tgatgtggac caataacatc acttatatgc
     2761 tgaagagtga aaacaaaacc acgggcgacc gtttgtcgat catcccggag tatacgttga
     2821 actcaacgct gagctggcag gcacgggaag atttgtcgat gcaaacgacc ttcacctggt
     2881 acggcaagca gcagccgaag aagtacaact ataaaggtca gccagcggtt ggaccggaaa
     2941 ccaaagaaat tagtccttac agcattgttg gcctgagcgc gacctgggat gtgacgaaga
     3001 atgtcagtct gaccggcggc gtggacaatc tgttcgacaa acgtttgtgg cgtgcgggta
     3061 atgcccagac cacgggcgat ttggcagggg ccaactatat cgccggtgcc ggggcgtata
     3121 cctataacga gccgggacgt acgtggtata tgagcgtaaa cacccacttc tgatactaga
     3181 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     3241 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     3301 tgcgtttata
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