BBa_J23115 1 BBa_J23115 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_I746000 1 BBa_I746000 AIP generator (agrB + agrD with RBSes) 2007-10-19T11:00:00Z 2015-08-31T04:08:04Z The original sequences for agrB and agrD were taken from the S Aureus Sequencing Project at Oklahoma University (http://www.genome.ou.edu/staph.html). The sequences were then codon-optimised for E. coli using GeneDesigner from DNA2.0 (http://www.dna20.com), and then synthesised by that company. This part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBirck-compatible signalling mechanism. In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the ''agr'' locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP. This part contains the coding regions for group I agrB and agrD (in that order), prefixed by the B0034 RBS and separated by a SaII site. false false _116_ 0 1851 9 Not in stock false N/A false David Wyatt annotation1950179 1 agrB coding sequence range1950179 1 19 591 annotation1950181 1 SaII site range1950181 1 592 597 annotation1950180 1 B0034-sequence RBS range1950180 1 598 609 annotation1950178 1 B0034-sequence RBS range1950178 1 1 12 annotation1950182 1 agrD coding sequence range1950182 1 616 759 BBa_I746010 1 BBa_I746010 AIP generator + outer membrane permeability 2007-10-22T11:00:00Z 2015-08-31T04:08:04Z Standard assembly. Intermediate - consists of AIP generator (I746000) + term + outer membrane porosity (using the mutant FepA device). false false _116_ 0 1851 9 It's complicated false N/A false David Wyatt component1953338 1 BBa_B0012 component1953344 1 BBa_B0034 component1953348 1 BBa_B0012 component1953342 1 BBa_J23115 component1953345 1 BBa_I746200 component1953335 1 BBa_I746000 component1953346 1 BBa_B0010 component1953336 1 BBa_B0010 annotation1953335 1 BBa_I746000 range1953335 1 1 759 annotation1953348 1 BBa_B0012 range1953348 1 3270 3310 annotation1953344 1 BBa_B0034 range1953344 1 948 959 annotation1953345 1 BBa_I746200 range1953345 1 966 3173 annotation1953342 1 BBa_J23115 range1953342 1 905 939 annotation1953336 1 BBa_B0010 range1953336 1 768 847 annotation1953346 1 BBa_B0010 range1953346 1 3182 3261 annotation1953338 1 BBa_B0012 range1953338 1 856 896 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I746200 1 BBa_I746200 FepA L8T Mutant - Large Diffusion pore for E. coli outer membrane. 2007-08-28T11:00:00Z 2015-08-31T04:08:04Z The mutants were developed by Dr Salete Newton working in Professor P. Klebba's lab at the university of Oklahoma. They were kindly sent to us by Professor Klebba. Released HQ 2013 FepA is an outer membrane receptor protein for Escherichia coli involved in the uptake of the ferric enterobactin siderophore. This part is a deletion mutant of the wild type FepA, which has been shown to convert the protein into an open diffusion channel, similar to a porin. It allows larger molecules through than normally would be permitted. false false _116_ 0 2118 9 In stock false The original mutants had an EcoR1 restriction site within their coding sequence. This had to be removed via site directed mutagenesis, using a megaprimer based method. true Narin Hengrung BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J23115_sequence 1 tttatagctagctcagcccttggtacaatgctagc BBa_B0034_sequence 1 aaagaggagaaa BBa_I746000_sequence 1 aaagaggagaaatactagatgaactattttgacaacaagatcgaccaatttgctacttacctgcaaaaacgcaacaacctggaccacatccagtttctgcaagttcgtctgggcatgcaggttctggctaaaaacatcggcaaactgatcgttatgtacaccatcgcctatattctgaacatcttcctgtttaccctgatcaccaacctgaccttctatctgattcgccgtcatgcccacggcgctcatgcaccttcttccttttggtgttatgtagagagcattattctgtttatcctgctgccgctggtaatcgttaacttccacatcaatttcctgattatgatcatcctgactgtgatttccctgggtgtaatctctgtttacgctccggctgccactaagaaaaagccgattccggtgcgcctgatcaaacgtaaaaagtactacgctatcatcgtttccctgaccctgttcattatcaccctgattatcaaagaaccgtttgcacagttcatccagctgggtattatcatcgaagcgatcactctgctgccgatctttttcatcaaagaggatctgaaataataagtcgacaaagaggagaaatactagatgaatactctgttcaatctgtttttcgatttcattactggcatcctgaagaacatcggtaacatcgcagcgtacagcacctgcgacttcatcatggatgaagtagaggtcccgaaagagctgacccagctgcatgaataataa BBa_I746200_sequence 1 atgaacaagaagattcattccctggccttgttggtcaatctggggatttatggggtagcgcaggcacaagagccgaccgatactcctgtttcacatgacgatactattgtcgttaccgccgccgagcagaacttacaggcgcctggcgtttcgaccatcaccgcagatgaaatccgcaaaaacccggttgcccgcgatgtgtcgaagatcatccgtaccatgccaggcgttaacctgaccggtaactccaccagtggtcagcgtgggaataaccgacagattgatattcgcggtatgggtccggaaaacacgctgattttgattgacggcaagccggtaagcagccgtaactcggtgcgtcagggctggcgtggcgagcgcgatacccgtggtgatacttcctgggtgccacctgaaatgattgaacgtattgaagttctgcgtggtccggcagctgcgcgttatggcaacggcgcggcgggcggcgtggttaacatcattaccaaaaaaggcagcggcgagtggcacggctcctgggacgcatatttcaatgcgccagaacataaagaggaaggtgccaccaaacgcactaactttagcctgaccggtccgctgggcgacgagttcagcttccgtttgtatggcaacctcgacaaaacccaggctgacgcgtgggatatcaaccagggccatcagtccgcgcgtgccggaacgtatgccacgacgttaccagccgggcgcgaaggggtaatcaacaaagatattaatggcgtggtgcgctgggatttcgcgccattgcaatcgctggaactggaagcaggttacagccgccagggtaacctgtatgcgggcgacacccagaataccaactccgattcctatacccgctcgaaatatggcgatgaaaccaaccgtctgtatcgccagaactacgcgctgacctggaacggtggctgggataacggcgtgaccaccagcaactgggtgcagtacgaacacacccgtaactcgcgtattccggaaggtctggcgggcggtaccgaagggaaatttaacgaaaaagcgacacaggatttcgtcgatatcgatcttgatgacgtgatgctgcacagcgaagttaacctgccgattgatttcctcgttaaccagacgctgacgctgggtacggagtggaatcagcaacggatgaaggacttaagttccaacacccaggcactgaccggaacgaataccggtggcgctattgatggcgtgagtaccaccgaccgtagcccgtattcaaaagcagaaattttctcgctgtttgccgaaaacaacatggagctgactgacagcaccatcgtaacgccggggctgcgtttcgatcatcacagtattgtcggcaataactggagcccggcgctgaacatatcgcaaggtttaggcgatgacttcacgctgaaaatgggcatcgcccgtgcttataaagcgccgagcctgtaccagactaacccgaactacattctctacagtaaaggtcagggttgctatgccagcgcgggcggctgctatctgcaaggtaacgatgacctgaaagcagaaaccagcatcaacaaagagattggtctggagttcaaacgcgacgggtggctggcgggcgtcacctggttccgtaacgattatcgcaataagattgaagcgggctatgtggctgtagggcaaaattgtgaaacagcggtggttgaaggtctggaaggatcgttaaacgtaccggttagcgaaacggtgatgtggaccaataacatcacttatatgctgaagagtgaaaacaaaaccacgggcgaccgtttgtcgatcatcccggagtatacgttgaactcaacgctgagctggcaggcacgggaagatttgtcgatgcaaacgaccttcacctggtacggcaagcagcagccgaagaagtacaactataaaggtcagccagcggttggaccggaaaccaaagaaattagtccttacagcattgttggcctgagcgcgacctgggatgtgacgaagaatgtcagtctgaccggcggcgtggacaatctgttcgacaaacgtttgtggcgtgcgggtaatgcccagaccacgggcgatttggcaggggccaactatatcgccggtgccggggcgtatacctataacgagccgggacgtacgtggtatatgagcgtaaacacccacttctga BBa_I746010_sequence 1 aaagaggagaaatactagatgaactattttgacaacaagatcgaccaatttgctacttacctgcaaaaacgcaacaacctggaccacatccagtttctgcaagttcgtctgggcatgcaggttctggctaaaaacatcggcaaactgatcgttatgtacaccatcgcctatattctgaacatcttcctgtttaccctgatcaccaacctgaccttctatctgattcgccgtcatgcccacggcgctcatgcaccttcttccttttggtgttatgtagagagcattattctgtttatcctgctgccgctggtaatcgttaacttccacatcaatttcctgattatgatcatcctgactgtgatttccctgggtgtaatctctgtttacgctccggctgccactaagaaaaagccgattccggtgcgcctgatcaaacgtaaaaagtactacgctatcatcgtttccctgaccctgttcattatcaccctgattatcaaagaaccgtttgcacagttcatccagctgggtattatcatcgaagcgatcactctgctgccgatctttttcatcaaagaggatctgaaataataagtcgacaaagaggagaaatactagatgaatactctgttcaatctgtttttcgatttcattactggcatcctgaagaacatcggtaacatcgcagcgtacagcacctgcgacttcatcatggatgaagtagaggtcccgaaagagctgacccagctgcatgaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttatagctagctcagcccttggtacaatgctagctactagagaaagaggagaaatactagatgaacaagaagattcattccctggccttgttggtcaatctggggatttatggggtagcgcaggcacaagagccgaccgatactcctgtttcacatgacgatactattgtcgttaccgccgccgagcagaacttacaggcgcctggcgtttcgaccatcaccgcagatgaaatccgcaaaaacccggttgcccgcgatgtgtcgaagatcatccgtaccatgccaggcgttaacctgaccggtaactccaccagtggtcagcgtgggaataaccgacagattgatattcgcggtatgggtccggaaaacacgctgattttgattgacggcaagccggtaagcagccgtaactcggtgcgtcagggctggcgtggcgagcgcgatacccgtggtgatacttcctgggtgccacctgaaatgattgaacgtattgaagttctgcgtggtccggcagctgcgcgttatggcaacggcgcggcgggcggcgtggttaacatcattaccaaaaaaggcagcggcgagtggcacggctcctgggacgcatatttcaatgcgccagaacataaagaggaaggtgccaccaaacgcactaactttagcctgaccggtccgctgggcgacgagttcagcttccgtttgtatggcaacctcgacaaaacccaggctgacgcgtgggatatcaaccagggccatcagtccgcgcgtgccggaacgtatgccacgacgttaccagccgggcgcgaaggggtaatcaacaaagatattaatggcgtggtgcgctgggatttcgcgccattgcaatcgctggaactggaagcaggttacagccgccagggtaacctgtatgcgggcgacacccagaataccaactccgattcctatacccgctcgaaatatggcgatgaaaccaaccgtctgtatcgccagaactacgcgctgacctggaacggtggctgggataacggcgtgaccaccagcaactgggtgcagtacgaacacacccgtaactcgcgtattccggaaggtctggcgggcggtaccgaagggaaatttaacgaaaaagcgacacaggatttcgtcgatatcgatcttgatgacgtgatgctgcacagcgaagttaacctgccgattgatttcctcgttaaccagacgctgacgctgggtacggagtggaatcagcaacggatgaaggacttaagttccaacacccaggcactgaccggaacgaataccggtggcgctattgatggcgtgagtaccaccgaccgtagcccgtattcaaaagcagaaattttctcgctgtttgccgaaaacaacatggagctgactgacagcaccatcgtaacgccggggctgcgtttcgatcatcacagtattgtcggcaataactggagcccggcgctgaacatatcgcaaggtttaggcgatgacttcacgctgaaaatgggcatcgcccgtgcttataaagcgccgagcctgtaccagactaacccgaactacattctctacagtaaaggtcagggttgctatgccagcgcgggcggctgctatctgcaaggtaacgatgacctgaaagcagaaaccagcatcaacaaagagattggtctggagttcaaacgcgacgggtggctggcgggcgtcacctggttccgtaacgattatcgcaataagattgaagcgggctatgtggctgtagggcaaaattgtgaaacagcggtggttgaaggtctggaaggatcgttaaacgtaccggttagcgaaacggtgatgtggaccaataacatcacttatatgctgaagagtgaaaacaaaaccacgggcgaccgtttgtcgatcatcccggagtatacgttgaactcaacgctgagctggcaggcacgggaagatttgtcgatgcaaacgaccttcacctggtacggcaagcagcagccgaagaagtacaactataaaggtcagccagcggttggaccggaaaccaaagaaattagtccttacagcattgttggcctgagcgcgacctgggatgtgacgaagaatgtcagtctgaccggcggcgtggacaatctgttcgacaaacgtttgtggcgtgcgggtaatgcccagaccacgggcgatttggcaggggccaactatatcgccggtgccggggcgtatacctataacgagccgggacgtacgtggtatatgagcgtaaacacccacttctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z