##gff-version 3
##sequence-region BBa_I746010 1 3310
BBa_I746010	.	terminator	768	847	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I746010	.	stem_loop	779	822	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I746010	.	engineered_region	768	847	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I746010	.	engineered_region	1	759	.	+	0	ID=BBa_I746000;Name=BBa_I746000
BBa_I746010	.	sequence_feature	592	597	.	+	0	ID=annotation1950181;Name=SaII site;Parent=BBa_I746000
BBa_I746010	.	ribosome_entry_site	1	12	.	+	0	ID=annotation1950178;Name=B0034-sequence RBS;Parent=BBa_I746000
BBa_I746010	.	CDS	616	759	.	+	0	ID=annotation1950182;Name=agrD coding sequence;Parent=BBa_I746000
BBa_I746010	.	CDS	19	591	.	+	0	ID=annotation1950179;Name=agrB coding sequence;Parent=BBa_I746000
BBa_I746010	.	ribosome_entry_site	598	609	.	+	0	ID=annotation1950180;Name=B0034-sequence RBS;Parent=BBa_I746000
BBa_I746010	.	terminator	3270	3310	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I746010	.	engineered_region	3270	3310	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I746010	.	stop_codon	3303	3303	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I746010	.	stem_loop	3277	3296	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I746010	.	polyA_site	3297	3310	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I746010	.	promoter	905	939	.	+	0	ID=BBa_J23115;Name=BBa_J23115
BBa_I746010	.	CDS	966	3173	.	+	0	ID=BBa_I746200;Name=BBa_I746200
BBa_I746010	.	terminator	3182	3261	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I746010	.	stem_loop	3193	3236	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I746010	.	engineered_region	3182	3261	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I746010	.	terminator	856	896	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I746010	.	engineered_region	856	896	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I746010	.	stop_codon	889	889	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I746010	.	stem_loop	863	882	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I746010	.	polyA_site	883	896	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I746010	.	ribosome_entry_site	948	959	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I746010	.	conserved_region	952	955	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I746010
aaagaggagaaatactagatgaactattttgacaacaagatcgaccaatttgctacttacctgcaaaaacgcaacaacc
tggaccacatccagtttctgcaagttcgtctgggcatgcaggttctggctaaaaacatcggcaaactgatcgttatgta
caccatcgcctatattctgaacatcttcctgtttaccctgatcaccaacctgaccttctatctgattcgccgtcatgcc
cacggcgctcatgcaccttcttccttttggtgttatgtagagagcattattctgtttatcctgctgccgctggtaatcg
ttaacttccacatcaatttcctgattatgatcatcctgactgtgatttccctgggtgtaatctctgtttacgctccggc
tgccactaagaaaaagccgattccggtgcgcctgatcaaacgtaaaaagtactacgctatcatcgtttccctgaccctg
ttcattatcaccctgattatcaaagaaccgtttgcacagttcatccagctgggtattatcatcgaagcgatcactctgc
tgccgatctttttcatcaaagaggatctgaaataataagtcgacaaagaggagaaatactagatgaatactctgttcaa
tctgtttttcgatttcattactggcatcctgaagaacatcggtaacatcgcagcgtacagcacctgcgacttcatcatg
gatgaagtagaggtcccgaaagagctgacccagctgcatgaataataatactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagagtttatagctagctcagcccttggtacaatgctagctactagaga
aagaggagaaatactagatgaacaagaagattcattccctggccttgttggtcaatctggggatttatggggtagcgca
ggcacaagagccgaccgatactcctgtttcacatgacgatactattgtcgttaccgccgccgagcagaacttacaggcg
cctggcgtttcgaccatcaccgcagatgaaatccgcaaaaacccggttgcccgcgatgtgtcgaagatcatccgtacca
tgccaggcgttaacctgaccggtaactccaccagtggtcagcgtgggaataaccgacagattgatattcgcggtatggg
tccggaaaacacgctgattttgattgacggcaagccggtaagcagccgtaactcggtgcgtcagggctggcgtggcgag
cgcgatacccgtggtgatacttcctgggtgccacctgaaatgattgaacgtattgaagttctgcgtggtccggcagctg
cgcgttatggcaacggcgcggcgggcggcgtggttaacatcattaccaaaaaaggcagcggcgagtggcacggctcctg
ggacgcatatttcaatgcgccagaacataaagaggaaggtgccaccaaacgcactaactttagcctgaccggtccgctg
ggcgacgagttcagcttccgtttgtatggcaacctcgacaaaacccaggctgacgcgtgggatatcaaccagggccatc
agtccgcgcgtgccggaacgtatgccacgacgttaccagccgggcgcgaaggggtaatcaacaaagatattaatggcgt
ggtgcgctgggatttcgcgccattgcaatcgctggaactggaagcaggttacagccgccagggtaacctgtatgcgggc
gacacccagaataccaactccgattcctatacccgctcgaaatatggcgatgaaaccaaccgtctgtatcgccagaact
acgcgctgacctggaacggtggctgggataacggcgtgaccaccagcaactgggtgcagtacgaacacacccgtaactc
gcgtattccggaaggtctggcgggcggtaccgaagggaaatttaacgaaaaagcgacacaggatttcgtcgatatcgat
cttgatgacgtgatgctgcacagcgaagttaacctgccgattgatttcctcgttaaccagacgctgacgctgggtacgg
agtggaatcagcaacggatgaaggacttaagttccaacacccaggcactgaccggaacgaataccggtggcgctattga
tggcgtgagtaccaccgaccgtagcccgtattcaaaagcagaaattttctcgctgtttgccgaaaacaacatggagctg
actgacagcaccatcgtaacgccggggctgcgtttcgatcatcacagtattgtcggcaataactggagcccggcgctga
acatatcgcaaggtttaggcgatgacttcacgctgaaaatgggcatcgcccgtgcttataaagcgccgagcctgtacca
gactaacccgaactacattctctacagtaaaggtcagggttgctatgccagcgcgggcggctgctatctgcaaggtaac
gatgacctgaaagcagaaaccagcatcaacaaagagattggtctggagttcaaacgcgacgggtggctggcgggcgtca
cctggttccgtaacgattatcgcaataagattgaagcgggctatgtggctgtagggcaaaattgtgaaacagcggtggt
tgaaggtctggaaggatcgttaaacgtaccggttagcgaaacggtgatgtggaccaataacatcacttatatgctgaag
agtgaaaacaaaaccacgggcgaccgtttgtcgatcatcccggagtatacgttgaactcaacgctgagctggcaggcac
gggaagatttgtcgatgcaaacgaccttcacctggtacggcaagcagcagccgaagaagtacaactataaaggtcagcc
agcggttggaccggaaaccaaagaaattagtccttacagcattgttggcctgagcgcgacctgggatgtgacgaagaat
gtcagtctgaccggcggcgtggacaatctgttcgacaaacgtttgtggcgtgcgggtaatgcccagaccacgggcgatt
tggcaggggccaactatatcgccggtgccggggcgtatacctataacgagccgggacgtacgtggtatatgagcgtaaa
cacccacttctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata