LOCUS BBa_I746103 2161 bp DNA linear 22-Nov-2024 DEFINITION I746101 (AIP sensor infrastructure - agrC + agrA with RBSes + Terminator) + I746104 (Inducible P2) ACCESSION BBa_I746103 VERSION BBa_I746103.1 FEATURES Location/Qualifiers RBS 1..12 /label=B0034-sequence RBS RBS 1273..1284 /label=B0034-sequence RBS misc_feature 1267..1272 /label=BamHI site CDS 19..1266 /label=agrC coding sequence CDS 1291..1920 /label=agrA coding sequence misc_feature 1..1920 /label=BBa_I746100 stem_loop 1940..1983 /label=stem_loop misc_feature 1929..2008 /label=BBa_B0010 terminator 1929..2008 /label=BBa_B0010 stem_loop 2024..2043 /label=T7 TE misc_feature 2050..2050 /label=stop polyA_site 2044..2057 /label=polya misc_feature 2017..2057 /label=BBa_B0012 terminator 2017..2057 /label=BBa_B0012 promoter 2066..2161 /label=BBa_I746104 ORIGIN 1 aaagaggaga aatactagat gattctgatg ttcaccattc cagcaattat tagcggtatt 61 aagtacagca agctggatta tttcttcatc atcgtaatct ctaccctgtc cctgttcctg 121 ttcaaaatgt tcgattccgc gagcctgatt atcctgactt ccttcatcat catcatgtat 181 ttcgtaaaaa tcaaatggta tagcatcctg ctgatcatga cctcccagat tatcctgtac 241 tgtgccaact acatgtatat cgttatctac gcgtacatca cgaaaatctc cgattctatc 301 ttcgttatct ttccgtcttt cttcgtggtg tacgttacga tctctattct gttttcctat 361 attatcaatc gcgttctgaa aaagatctct actccgtacc tgattctgaa caaaggtttt 421 ctgatcgtca tctccactat cctgctgctg accttctctc tgttcttctt ttactctcag 481 atcaactccg atgaggcaaa agtcatccgt cagtacagct tcatctttat cggtattact 541 atcttcctga gcattctgac tttcgttatc agccagttcc tgctgaaaga aatgaagtac 601 aagcgcaacc aagaagaaat cgagacttac tacgagtaca ccctgaagat tgaagcgatc 661 aacaacgaaa tgcgtaaatt tcgtcacgac tacgttaaca ttctgaccac cctgagcgaa 721 tacatccgtg aagacgacat gcctggcctg cgtgattatt tcaacaaaaa cattgtgccg 781 atgaaagata acctgcaaat gaacgctatt aagctgaacg gtatcgaaaa cctgaaagta 841 cgtgagatca agggtctgat taccgctaaa atcctgcgtg cgcaggaaat gaacatccca 901 attagcatcg aaatcccaga cgaagtttct agcattaacc tgaacatgat tgacctgtct 961 cgtagcatcg gcatcatcct ggacaacgct atcgaagctt ccactgaaat cgacgacccg 1021 attattcgcg ttgcttttat cgagagcgaa aactccgtga cctttatcgt gatgaacaaa 1081 tgcgcggacg atatcccgcg tattcacgaa ctgttccagg aatctttcag caccaagggt 1141 gaaggtcgcg gcctgggcct gtccaccctg aaagagatcg cggataacgc cgacaacgtt 1201 ctgctggaca ctattatcga aaacggtttc ttcattcaga aagtcgaaat catcaataac 1261 taataaggat ccaaagagga gaaatactag atggaaatcg cactggctac ggacaatcca 1321 tatgaggttc tggaacaggc taaaaacatg aacgacatcg gctgctattt cctggatatt 1381 cagctgagca ccgacatcaa cggtatcaaa ctgggctctg aaatccgtaa acatgatccg 1441 gttggtaaca ttattttcgt gacttctcat tctgagctga cctatctgac gttcgtatac 1501 aaggttgcag ccatggattt catctttaaa gacgatccgg cagagctgcg tacgcgcatc 1561 atcgactgtc tggaaactgc gcacacgcgc ctgcaactgc tgtccaaaga taactccgtg 1621 gaaaccattg aactgaaacg tggcagcaac agcgtttacg ttcagtacga tgatatcatg 1681 ttcttcgaaa gctccactaa gtcccaccgt ctgatcgcgc atctggataa tcgtcagatc 1741 gagttctatg gcaacctgaa agagctgagc cagctggacg atcgcttctt ccgttgtcat 1801 aactccttcg ttgttaaccg tcacaacatc gaatccatcg actccaaaga gcgtattgta 1861 tacttcaaga acaaagaaca ctgttatgcg tctgtacgca atgtcaagaa aatctaataa 1921 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 1981 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 2041 gcctttctgc gtttatatac tagagattaa atacaaatta catttaacag ttaagtattt 2101 atttcctaca gttaggcaat ataatgataa aagattgtac taaatcgtat aatgacagtg 2161 a //