LOCUS BBa_I746202 2406 bp DNA linear 20-Dec-2024 DEFINITION Outer Membrane Permeability Device ACCESSION BBa_I746202 VERSION BBa_I746202.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..2269 /label=BBa_I746200 stem_loop 2289..2332 /label=stem_loop misc_feature 2278..2357 /label=BBa_B0010 terminator 2278..2357 /label=BBa_B0010 stem_loop 2373..2392 /label=T7 TE misc_feature 2399..2399 /label=stop polyA_site 2393..2406 /label=polya misc_feature 2366..2406 /label=BBa_B0012 terminator 2366..2406 /label=BBa_B0012 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatgaacaag aagattcatt ccctggcctt gttggtcaat ctggggattt atggggtagc 121 gcaggcacaa gagccgaccg atactcctgt ttcacatgac gatactattg tcgttaccgc 181 cgccgagcag aacttacagg cgcctggcgt ttcgaccatc accgcagatg aaatccgcaa 241 aaacccggtt gcccgcgatg tgtcgaagat catccgtacc atgccaggcg ttaacctgac 301 cggtaactcc accagtggtc agcgtgggaa taaccgacag attgatattc gcggtatggg 361 tccggaaaac acgctgattt tgattgacgg caagccggta agcagccgta actcggtgcg 421 tcagggctgg cgtggcgagc gcgatacccg tggtgatact tcctgggtgc cacctgaaat 481 gattgaacgt attgaagttc tgcgtggtcc ggcagctgcg cgttatggca acggcgcggc 541 gggcggcgtg gttaacatca ttaccaaaaa aggcagcggc gagtggcacg gctcctggga 601 cgcatatttc aatgcgccag aacataaaga ggaaggtgcc accaaacgca ctaactttag 661 cctgaccggt ccgctgggcg acgagttcag cttccgtttg tatggcaacc tcgacaaaac 721 ccaggctgac gcgtgggata tcaaccaggg ccatcagtcc gcgcgtgccg gaacgtatgc 781 cacgacgtta ccagccgggc gcgaaggggt aatcaacaaa gatattaatg gcgtggtgcg 841 ctgggatttc gcgccattgc aatcgctgga actggaagca ggttacagcc gccagggtaa 901 cctgtatgcg ggcgacaccc agaataccaa ctccgattcc tatacccgct cgaaatatgg 961 cgatgaaacc aaccgtctgt atcgccagaa ctacgcgctg acctggaacg gtggctggga 1021 taacggcgtg accaccagca actgggtgca gtacgaacac acccgtaact cgcgtattcc 1081 ggaaggtctg gcgggcggta ccgaagggaa atttaacgaa aaagcgacac aggatttcgt 1141 cgatatcgat cttgatgacg tgatgctgca cagcgaagtt aacctgccga ttgatttcct 1201 cgttaaccag acgctgacgc tgggtacgga gtggaatcag caacggatga aggacttaag 1261 ttccaacacc caggcactga ccggaacgaa taccggtggc gctattgatg gcgtgagtac 1321 caccgaccgt agcccgtatt caaaagcaga aattttctcg ctgtttgccg aaaacaacat 1381 ggagctgact gacagcacca tcgtaacgcc ggggctgcgt ttcgatcatc acagtattgt 1441 cggcaataac tggagcccgg cgctgaacat atcgcaaggt ttaggcgatg acttcacgct 1501 gaaaatgggc atcgcccgtg cttataaagc gccgagcctg taccagacta acccgaacta 1561 cattctctac agtaaaggtc agggttgcta tgccagcgcg ggcggctgct atctgcaagg 1621 taacgatgac ctgaaagcag aaaccagcat caacaaagag attggtctgg agttcaaacg 1681 cgacgggtgg ctggcgggcg tcacctggtt ccgtaacgat tatcgcaata agattgaagc 1741 gggctatgtg gctgtagggc aaaattgtga aacagcggtg gttgaaggtc tggaaggatc 1801 gttaaacgta ccggttagcg aaacggtgat gtggaccaat aacatcactt atatgctgaa 1861 gagtgaaaac aaaaccacgg gcgaccgttt gtcgatcatc ccggagtata cgttgaactc 1921 aacgctgagc tggcaggcac gggaagattt gtcgatgcaa acgaccttca cctggtacgg 1981 caagcagcag ccgaagaagt acaactataa aggtcagcca gcggttggac cggaaaccaa 2041 agaaattagt ccttacagca ttgttggcct gagcgcgacc tgggatgtga cgaagaatgt 2101 cagtctgacc ggcggcgtgg acaatctgtt cgacaaacgt ttgtggcgtg cgggtaatgc 2161 ccagaccacg ggcgatttgg caggggccaa ctatatcgcc ggtgccgggg cgtataccta 2221 taacgagccg ggacgtacgt ggtatatgag cgtaaacacc cacttctgat actagagcca 2281 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2341 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2401 tttata //