LOCUS       BBa_I746204             2397 bp    DNA     linear       26-Dec-2024
DEFINITION  FepA L8 Mutant with Elowitz RBS and Standard Terminator
ACCESSION   BBa_I746204
VERSION     BBa_I746204.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             21..2260
                     /label=BBa_I746200
     stem_loop       2280..2323
                     /label=stem_loop
     misc_feature    2269..2348
                     /label=BBa_B0010
     terminator      2269..2348
                     /label=BBa_B0010
     stem_loop       2364..2383
                     /label=T7 TE
     misc_feature    2390..2390
                     /label=stop
     polyA_site      2384..2397
                     /label=polya
     misc_feature    2357..2397
                     /label=BBa_B0012
     terminator      2357..2397
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagag gaattcgcgg ccgcttctag atgaacaaga agattcattc
       61 cctggccttg ttggtcaatc tggggattta tggggtagcg caggcacaag agccgaccga
      121 tactcctgtt tcacatgacg atactattgt cgttaccgcc gccgagcaga acttacaggc
      181 gcctggcgtt tcgaccatca ccgcagatga aatccgcaaa aacccggttg cccgcgatgt
      241 gtcgaagatc atccgtacca tgccaggcgt taacctgacc ggtaactcca ccagtggtca
      301 gcgtgggaat aaccgacaga ttgatattcg cggtatgggt ccggaaaaca cgctgatttt
      361 gattgacggc aagccggtaa gcagccgtaa ctcggtgcgt cagggctggc gtggcgagcg
      421 cgatacccgt ggtgatactt cctgggtgcc acctgaaatg attgaacgta ttgaagttct
      481 gcgtggtccg gcagctgcgc gttatggcaa cggcgcggcg ggcggcgtgg ttaacatcat
      541 taccaaaaaa ggcagcggcg agtggcacgg ctcctgggac gcatatttca atgcgccaga
      601 acataaagag gaaggtgcca ccaaacgcac taactttagc ctgaccggtc cgctgggcga
      661 cgagttcagc ttccgtttgt atggcaacct cgacaaaacc caggctgacg cgtgggatat
      721 caaccagggc catcagtccg cgcgtgccgg aacgtatgcc acgacgttac cagccgggcg
      781 cgaaggggta atcaacaaag atattaatgg cgtggtgcgc tgggatttcg cgccattgca
      841 atcgctggaa ctggaagcag gttacagccg ccagggtaac ctgtatgcgg gcgacaccca
      901 gaataccaac tccgattcct atacccgctc gaaatatggc gatgaaacca accgtctgta
      961 tcgccagaac tacgcgctga cctggaacgg tggctgggat aacggcgtga ccaccagcaa
     1021 ctgggtgcag tacgaacaca cccgtaactc gcgtattccg gaaggtctgg cgggcggtac
     1081 cgaagggaaa tttaacgaaa aagcgacaca ggatttcgtc gatatcgatc ttgatgacgt
     1141 gatgctgcac agcgaagtta acctgccgat tgatttcctc gttaaccaga cgctgacgct
     1201 gggtacggag tggaatcagc aacggatgaa ggacttaagt tccaacaccc aggcactgac
     1261 cggaacgaat accggtggcg ctattgatgg cgtgagtacc accgaccgta gcccgtattc
     1321 aaaagcagaa attttctcgc tgtttgccga aaacaacatg gagctgactg acagcaccat
     1381 cgtaacgccg gggctgcgtt tcgatcatca cagtattgtc ggcaataact ggagcccggc
     1441 gctgaacata tcgcaaggtt taggcgatga cttcacgctg aaaatgggca tcgcccgtgc
     1501 ttataaagcg ccgagcctgt accagactaa cccgaactac attctctaca gtaaaggtca
     1561 gggttgctat gccagcgcgg gcggctgcta tctgcaaggt aacgatgacc tgaaagcaga
     1621 aaccagcatc aacaaagaga ttggtctgga gttcaaacgc gacgggtggc tggcgggcgg
     1681 ctatgtggct gtagggcaaa acgcagtcgg caccgatctc tatcagtggg ataacgtgcc
     1741 gaaagcggtg gttgaaggtc tggaaggatc gttaaacgta ccggttagcg aaacggtgat
     1801 gtggaccaat aacatcactt atatgctgaa gagtgaaaac aaaaccacgg gcgaccgttt
     1861 gtcgatcatc ccggagtata cgttgaactc aacgctgagc tggcaggcac gggaagattt
     1921 gtcgatgcaa acgaccttca cctggtacgg caagcagcag ccgaagaagt acaactataa
     1981 aggtcagcca gcggttggac cggaaaccaa agaaattagt ccttacagca ttgttggcct
     2041 gagcgcgacc tgggatgtga cgaagaatgt cagtctgacc ggcggcgtgg acaatctgtt
     2101 cgacaaacgt ttgtggcgtg cgggtaatgc ccagaccacg ggcgatttgg caggggccaa
     2161 ctatatcgcc ggtgccgggg cgtataccta taacgagccg ggacgtacgt ggtatatgag
     2221 cgtaaacacc cacttctgat actagtagcg gccgctgcag tactagagcc aggcatcaaa
     2281 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     2341 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//