LOCUS BBa_I746211 3353 bp DNA linear 15-Dec-2024 DEFINITION AIP Sender ACCESSION BBa_I746211 VERSION BBa_I746211.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23115 RBS 44..55 /label=B0034-sequence RBS CDS 62..634 /label=agrB coding sequence RBS 641..652 /label=B0034-sequence RBS misc_feature 635..640 /label=SaII site CDS 659..802 /label=agrD coding sequence misc_feature 44..802 /label=BBa_I746000 stem_loop 822..865 /label=stem_loop misc_feature 811..890 /label=BBa_B0010 terminator 811..890 /label=BBa_B0010 stem_loop 906..925 /label=T7 TE misc_feature 932..932 /label=stop polyA_site 926..939 /label=polya misc_feature 899..939 /label=BBa_B0012 terminator 899..939 /label=BBa_B0012 promoter 948..982 /label=BBa_J23115 misc_feature 995..998 /label=conserved RBS 991..1002 /label=BBa_B0034 CDS 1009..3216 /label=BBa_I746200 stem_loop 3236..3279 /label=stem_loop misc_feature 3225..3304 /label=BBa_B0010 terminator 3225..3304 /label=BBa_B0010 stem_loop 3320..3339 /label=T7 TE misc_feature 3346..3346 /label=stop polyA_site 3340..3353 /label=polya misc_feature 3313..3353 /label=BBa_B0012 terminator 3313..3353 /label=BBa_B0012 ORIGIN 1 tttatagcta gctcagccct tggtacaatg ctagctacta gagaaagagg agaaatacta 61 gatgaactat tttgacaaca agatcgacca atttgctact tacctgcaaa aacgcaacaa 121 cctggaccac atccagtttc tgcaagttcg tctgggcatg caggttctgg ctaaaaacat 181 cggcaaactg atcgttatgt acaccatcgc ctatattctg aacatcttcc tgtttaccct 241 gatcaccaac ctgaccttct atctgattcg ccgtcatgcc cacggcgctc atgcaccttc 301 ttccttttgg tgttatgtag agagcattat tctgtttatc ctgctgccgc tggtaatcgt 361 taacttccac atcaatttcc tgattatgat catcctgact gtgatttccc tgggtgtaat 421 ctctgtttac gctccggctg ccactaagaa aaagccgatt ccggtgcgcc tgatcaaacg 481 taaaaagtac tacgctatca tcgtttccct gaccctgttc attatcaccc tgattatcaa 541 agaaccgttt gcacagttca tccagctggg tattatcatc gaagcgatca ctctgctgcc 601 gatctttttc atcaaagagg atctgaaata ataagtcgac aaagaggaga aatactagat 661 gaatactctg ttcaatctgt ttttcgattt cattactggc atcctgaaga acatcggtaa 721 catcgcagcg tacagcacct gcgacttcat catggatgaa gtagaggtcc cgaaagagct 781 gacccagctg catgaataat aatactagag ccaggcatca aataaaacga aaggctcagt 841 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 901 acactggctc accttcgggt gggcctttct gcgtttatat actagagttt atagctagct 961 cagcccttgg tacaatgcta gctactagag aaagaggaga aatactagat gaacaagaag 1021 attcattccc tggccttgtt ggtcaatctg gggatttatg gggtagcgca ggcacaagag 1081 ccgaccgata ctcctgtttc acatgacgat actattgtcg ttaccgccgc cgagcagaac 1141 ttacaggcgc ctggcgtttc gaccatcacc gcagatgaaa tccgcaaaaa cccggttgcc 1201 cgcgatgtgt cgaagatcat ccgtaccatg ccaggcgtta acctgaccgg taactccacc 1261 agtggtcagc gtgggaataa ccgacagatt gatattcgcg gtatgggtcc ggaaaacacg 1321 ctgattttga ttgacggcaa gccggtaagc agccgtaact cggtgcgtca gggctggcgt 1381 ggcgagcgcg atacccgtgg tgatacttcc tgggtgccac ctgaaatgat tgaacgtatt 1441 gaagttctgc gtggtccggc agctgcgcgt tatggcaacg gcgcggcggg cggcgtggtt 1501 aacatcatta ccaaaaaagg cagcggcgag tggcacggct cctgggacgc atatttcaat 1561 gcgccagaac ataaagagga aggtgccacc aaacgcacta actttagcct gaccggtccg 1621 ctgggcgacg agttcagctt ccgtttgtat ggcaacctcg acaaaaccca ggctgacgcg 1681 tgggatatca accagggcca tcagtccgcg cgtgccggaa cgtatgccac gacgttacca 1741 gccgggcgcg aaggggtaat caacaaagat attaatggcg tggtgcgctg ggatttcgcg 1801 ccattgcaat cgctggaact ggaagcaggt tacagccgcc agggtaacct gtatgcgggc 1861 gacacccaga ataccaactc cgattcctat acccgctcga aatatggcga tgaaaccaac 1921 cgtctgtatc gccagaacta cgcgctgacc tggaacggtg gctgggataa cggcgtgacc 1981 accagcaact gggtgcagta cgaacacacc cgtaactcgc gtattccgga aggtctggcg 2041 ggcggtaccg aagggaaatt taacgaaaaa gcgacacagg atttcgtcga tatcgatctt 2101 gatgacgtga tgctgcacag cgaagttaac ctgccgattg atttcctcgt taaccagacg 2161 ctgacgctgg gtacggagtg gaatcagcaa cggatgaagg acttaagttc caacacccag 2221 gcactgaccg gaacgaatac cggtggcgct attgatggcg tgagtaccac cgaccgtagc 2281 ccgtattcaa aagcagaaat tttctcgctg tttgccgaaa acaacatgga gctgactgac 2341 agcaccatcg taacgccggg gctgcgtttc gatcatcaca gtattgtcgg caataactgg 2401 agcccggcgc tgaacatatc gcaaggttta ggcgatgact tcacgctgaa aatgggcatc 2461 gcccgtgctt ataaagcgcc gagcctgtac cagactaacc cgaactacat tctctacagt 2521 aaaggtcagg gttgctatgc cagcgcgggc ggctgctatc tgcaaggtaa cgatgacctg 2581 aaagcagaaa ccagcatcaa caaagagatt ggtctggagt tcaaacgcga cgggtggctg 2641 gcgggcgtca cctggttccg taacgattat cgcaataaga ttgaagcggg ctatgtggct 2701 gtagggcaaa attgtgaaac agcggtggtt gaaggtctgg aaggatcgtt aaacgtaccg 2761 gttagcgaaa cggtgatgtg gaccaataac atcacttata tgctgaagag tgaaaacaaa 2821 accacgggcg accgtttgtc gatcatcccg gagtatacgt tgaactcaac gctgagctgg 2881 caggcacggg aagatttgtc gatgcaaacg accttcacct ggtacggcaa gcagcagccg 2941 aagaagtaca actataaagg tcagccagcg gttggaccgg aaaccaaaga aattagtcct 3001 tacagcattg ttggcctgag cgcgacctgg gatgtgacga agaatgtcag tctgaccggc 3061 ggcgtggaca atctgttcga caaacgtttg tggcgtgcgg gtaatgccca gaccacgggc 3121 gatttggcag gggccaacta tatcgccggt gccggggcgt atacctataa cgagccggga 3181 cgtacgtggt atatgagcgt aaacacccac ttctgatact agagccaggc atcaaataaa 3241 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 3301 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //