BBa_I746211
type: ComponentDefinition
persistent identity: BBa I746211
display id: BBa_I746211
version: 1
was generated by: iGEM to SBOL conversion
description: AIP Sender
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: Standard assembly.
owned by: james
owned by: myers
mutable description: This part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism. In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP. In this part, the AIP generating part I746001 is combined with the permeability device, I746201, so that the AIP produced is able to leave the periplasm of the cell.
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 1851
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: David Wyatt
type: DnaRegion
role: partType/Signalling
role: SO:0000804
component: BBa_J23115
component: BBa_B0012
component: BBa_B0010
component: BBa_I746000
component: BBa_I746200
component: BBa_B0034
sequence annotation: BBa_J23115
sequence annotation: BBa_I746000
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_I746200
sequence: https://synbiohub.org/public/igem/BBa_I746211_sequence/1

BBa_J23115
type: Component
persistent identity: component1953352
display id: component1953352
version: 1
top level: BBa_I746211
definition: BBa_J23115
access: public

BBa_J23115
type: Component
persistent identity: component1953365
display id: component1953365
version: 1
top level: BBa_I746211
definition: BBa_J23115
access: public

BBa_B0012
type: Component
persistent identity: component1953361
display id: component1953361
version: 1
top level: BBa_I746211
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1953359
display id: component1953359
version: 1
top level: BBa_I746211
definition: BBa_B0010
access: public

BBa_I746000
type: Component
persistent identity: component1953358
display id: component1953358
version: 1
top level: BBa_I746211
definition: BBa_I746000
access: public

BBa_B0012
type: Component
persistent identity: component1953371
display id: component1953371
version: 1
top level: BBa_I746211
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1953369
display id: component1953369
version: 1
top level: BBa_I746211
definition: BBa_B0010
access: public

BBa_I746200
type: Component
persistent identity: component1953368
display id: component1953368
version: 1
top level: BBa_I746211
definition: BBa_I746200
access: public

BBa_B0034
type: Component
persistent identity: component1953367
display id: component1953367
version: 1
top level: BBa_I746211
definition: BBa_B0034
access: public

BBa_J23115
type: SequenceAnnotation
persistent identity: annotation1953352
display id: annotation1953352
version: 1
top level: BBa_I746211
location: https://synbiohub.org/public/igem/BBa_I746211/annotation1953352/range1953352/1
component: BBa_J23115

https://synbiohub.org/public/igem/BBa_I746211/annotation1953352/range1953352/1
type: Range
persistent identity: range1953352
display id: range1953352
version: 1
top level: BBa_I746211
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_I746000
type: SequenceAnnotation
persistent identity: annotation1953358
display id: annotation1953358
version: 1
top level: BBa_I746211
location: https://synbiohub.org/public/igem/BBa_I746211/annotation1953358/range1953358/1
component: BBa_I746000

https://synbiohub.org/public/igem/BBa_I746211/annotation1953358/range1953358/1
type: Range
persistent identity: range1953358
display id: range1953358
version: 1
top level: BBa_I746211
direction: direction/reverse
start: 44
end: 802
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1953359
display id: annotation1953359
version: 1
top level: BBa_I746211
location: https://synbiohub.org/public/igem/BBa_I746211/annotation1953359/range1953359/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746211/annotation1953359/range1953359/1
type: Range
persistent identity: range1953359
display id: range1953359
version: 1
top level: BBa_I746211
direction: direction/forward
start: 811
end: 890
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1953361
display id: annotation1953361
version: 1
top level: BBa_I746211
location: https://synbiohub.org/public/igem/BBa_I746211/annotation1953361/range1953361/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746211/annotation1953361/range1953361/1
type: Range
persistent identity: range1953361
display id: range1953361
version: 1
top level: BBa_I746211
direction: direction/forward
start: 899
end: 939
orientation: inline

BBa_J23115
type: SequenceAnnotation
persistent identity: annotation1953365
display id: annotation1953365
version: 1
top level: BBa_I746211
location: https://synbiohub.org/public/igem/BBa_I746211/annotation1953365/range1953365/1
component: BBa_J23115

https://synbiohub.org/public/igem/BBa_I746211/annotation1953365/range1953365/1
type: Range
persistent identity: range1953365
display id: range1953365
version: 1
top level: BBa_I746211
direction: direction/reverse
start: 948
end: 982
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1953367
display id: annotation1953367
version: 1
top level: BBa_I746211
location: https://synbiohub.org/public/igem/BBa_I746211/annotation1953367/range1953367/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I746211/annotation1953367/range1953367/1
type: Range
persistent identity: range1953367
display id: range1953367
version: 1
top level: BBa_I746211
direction: direction/reverse
start: 991
end: 1002
orientation: inline

BBa_I746200
type: SequenceAnnotation
persistent identity: annotation1953368
display id: annotation1953368
version: 1
top level: BBa_I746211
location: https://synbiohub.org/public/igem/BBa_I746211/annotation1953368/range1953368/1
component: BBa_I746200

https://synbiohub.org/public/igem/BBa_I746211/annotation1953368/range1953368/1
type: Range
persistent identity: range1953368
display id: range1953368
version: 1
top level: BBa_I746211
direction: direction/reverse
start: 1009
end: 3216
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1953369
display id: annotation1953369
version: 1
top level: BBa_I746211
location: https://synbiohub.org/public/igem/BBa_I746211/annotation1953369/range1953369/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746211/annotation1953369/range1953369/1
type: Range
persistent identity: range1953369
display id: range1953369
version: 1
top level: BBa_I746211
direction: direction/forward
start: 3225
end: 3304
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1953371
display id: annotation1953371
version: 1
top level: BBa_I746211
location: https://synbiohub.org/public/igem/BBa_I746211/annotation1953371/range1953371/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746211/annotation1953371/range1953371/1
type: Range
persistent identity: range1953371
display id: range1953371
version: 1
top level: BBa_I746211
direction: direction/forward
start: 3313
end: 3353
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_I746000
type: ComponentDefinition
persistent identity: BBa I746000
display id: BBa_I746000
version: 1
was generated by: iGEM to SBOL conversion
description: AIP generator (agrB + agrD with RBSes)
created: 2007-10-19T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: The original sequences for agrB and agrD were taken from the S Aureus Sequencing Project at Oklahoma University (http://www.genome.ou.edu/staph.html). The sequences were then codon-optimised for E. coli using GeneDesigner from DNA2.0 (http://www.dna20.com), and then synthesised by that company.
owned by: james
owned by: myers
mutable description: This part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBirck-compatible signalling mechanism. In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the ''agr'' locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP. This part contains the coding regions for group I agrB and agrD (in that order), prefixed by the B0034 RBS and separated by a SaII site.
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 1851
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: David Wyatt
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence annotation: B0034-sequence RBS
sequence annotation: agrB coding sequence
sequence annotation: SaII site
sequence annotation: agrD coding sequence
sequence: https://synbiohub.org/public/igem/BBa_I746000_sequence/1

B0034-sequence RBS
type: SequenceAnnotation
persistent identity: annotation1950178
display id: annotation1950178
version: 1
top level: BBa_I746000
location: https://synbiohub.org/public/igem/BBa_I746000/annotation1950178/range1950178/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_I746000/annotation1950178/range1950178/1
type: Range
persistent identity: range1950178
display id: range1950178
version: 1
top level: BBa_I746000
direction: direction/forward
start: 1
end: 12
orientation: inline

agrB coding sequence
type: SequenceAnnotation
persistent identity: annotation1950179
display id: annotation1950179
version: 1
top level: BBa_I746000
location: https://synbiohub.org/public/igem/BBa_I746000/annotation1950179/range1950179/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I746000/annotation1950179/range1950179/1
type: Range
persistent identity: range1950179
display id: range1950179
version: 1
top level: BBa_I746000
direction: direction/forward
start: 19
end: 591
orientation: inline

SaII site
type: SequenceAnnotation
persistent identity: annotation1950181
display id: annotation1950181
version: 1
top level: BBa_I746000
location: https://synbiohub.org/public/igem/BBa_I746000/annotation1950181/range1950181/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I746000/annotation1950181/range1950181/1
type: Range
persistent identity: range1950181
display id: range1950181
version: 1
top level: BBa_I746000
direction: direction/forward
start: 592
end: 597
orientation: inline

B0034-sequence RBS
type: SequenceAnnotation
persistent identity: annotation1950180
display id: annotation1950180
version: 1
top level: BBa_I746000
location: https://synbiohub.org/public/igem/BBa_I746000/annotation1950180/range1950180/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_I746000/annotation1950180/range1950180/1
type: Range
persistent identity: range1950180
display id: range1950180
version: 1
top level: BBa_I746000
direction: direction/forward
start: 598
end: 609
orientation: inline

agrD coding sequence
type: SequenceAnnotation
persistent identity: annotation1950182
display id: annotation1950182
version: 1
top level: BBa_I746000
location: https://synbiohub.org/public/igem/BBa_I746000/annotation1950182/range1950182/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I746000/annotation1950182/range1950182/1
type: Range
persistent identity: range1950182
display id: range1950182
version: 1
top level: BBa_I746000
direction: direction/forward
start: 616
end: 759
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_I746200
type: ComponentDefinition
persistent identity: BBa I746200
display id: BBa_I746200
version: 1
was generated by: iGEM to SBOL conversion
description: FepA L8T Mutant - Large Diffusion pore for E. coli outer membrane.
created: 2007-08-28T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: The mutants were developed by Dr Salete Newton working in Professor P. Klebba's lab at the university of Oklahoma. They were kindly sent to us by Professor Klebba.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: FepA is an outer membrane receptor protein for Escherichia coli involved in the uptake of the ferric enterobactin siderophore. This part is a deletion mutant of the wild type FepA, which has been shown to convert the protein into an open diffusion channel, similar to a porin. It allows larger molecules through than normally would be permitted.
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2118
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The original mutants had an EcoR1 restriction site within their coding sequence. This had to be removed via site directed mutagenesis, using a megaprimer based method.
star: true
creator: Narin Hengrung
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_I746200_sequence/1

BBa_J23115
type: ComponentDefinition
persistent identity: BBa J23115
display id: BBa_J23115
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23115_sequence/1

https://synbiohub.org/public/igem/BBa_I746211_sequence/1
type: Sequence
persistent identity: BBa I746211 sequence
display id: BBa_I746211_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatagctagctcagcccttggtacaatgctagctactagagaaagaggagaaatactagatgaactattttgacaacaagatcgaccaatttgctacttacctgcaaaaacgcaacaacctggaccacatccagtttctgcaagttcgtctgggcatgcaggttctggctaaaaacatcggcaaactgatcgttatgtacaccatcgcctatattctgaacatcttcctgtttaccctgatcaccaacctgaccttctatctgattcgccgtcatgcccacggcgctcatgcaccttcttccttttggtgttatgtagagagcattattctgtttatcctgctgccgctggtaatcgttaacttccacatcaatttcctgattatgatcatcctgactgtgatttccctgggtgtaatctctgtttacgctccggctgccactaagaaaaagccgattccggtgcgcctgatcaaacgtaaaaagtactacgctatcatcgtttccctgaccctgttcattatcaccctgattatcaaagaaccgtttgcacagttcatccagctgggtattatcatcgaagcgatcactctgctgccgatctttttcatcaaagaggatctgaaataataagtcgacaaagaggagaaatactagatgaatactctgttcaatctgtttttcgatttcattactggcatcctgaagaacatcggtaacatcgcagcgtacagcacctgcgacttcatcatggatgaagtagaggtcccgaaagagctgacccagctgcatgaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttatagctagctcagcccttggtacaatgctagctactagagaaagaggagaaatactagatgaacaagaagattcattccctggccttgttggtcaatctggggatttatggggtagcgcaggcacaagagccgaccgatactcctgtttcacatgacgatactattgtcgttaccgccgccgagcagaacttacaggcgcctggcgtttcgaccatcaccgcagatgaaatccgcaaaaacccggttgcccgcgatgtgtcgaagatcatccgtaccatgccaggcgttaacctgaccggtaactccaccagtggtcagcgtgggaataaccgacagattgatattcgcggtatgggtccggaaaacacgctgattttgattgacggcaagccggtaagcagccgtaactcggtgcgtcagggctggcgtggcgagcgcgatacccgtggtgatacttcctgggtgccacctgaaatgattgaacgtattgaagttctgcgtggtccggcagctgcgcgttatggcaacggcgcggcgggcggcgtggttaacatcattaccaaaaaaggcagcggcgagtggcacggctcctgggacgcatatttcaatgcgccagaacataaagaggaaggtgccaccaaacgcactaactttagcctgaccggtccgctgggcgacgagttcagcttccgtttgtatggcaacctcgacaaaacccaggctgacgcgtgggatatcaaccagggccatcagtccgcgcgtgccggaacgtatgccacgacgttaccagccgggcgcgaaggggtaatcaacaaagatattaatggcgtggtgcgctgggatttcgcgccattgcaatcgctggaactggaagcaggttacagccgccagggtaacctgtatgcgggcgacacccagaataccaactccgattcctatacccgctcgaaatatggcgatgaaaccaaccgtctgtatcgccagaactacgcgctgacctggaacggtggctgggataacggcgtgaccaccagcaactgggtgcagta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746000_sequence/1
type: Sequence
persistent identity: BBa I746000 sequence
display id: BBa_I746000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaactattttgacaacaagatcgaccaatttgctacttacctgcaaaaacgcaacaacctggaccacatccagtttctgcaagttcgtctgggcatgcaggttctggctaaaaacatcggcaaactgatcgttatgtacaccatcgcctatattctgaacatcttcctgtttaccctgatcaccaacctgaccttctatctgattcgccgtcatgcccacggcgctcatgcaccttcttccttttggtgttatgtagagagcattattctgtttatcctgctgccgctggtaatcgttaacttccacatcaatttcctgattatgatcatcctgactgtgatttccctgggtgtaatctctgtttacgctccggctgccactaagaaaaagccgattccggtgcgcctgatcaaacgtaaaaagtactacgctatcatcgtttccctgaccctgttcattatcaccctgattatcaaagaaccgtttgcacagttcatccagctgggtattatcatcgaagcgatcactctgctgccgatctttttcatcaaagaggatctgaaataataagtcgacaaagaggagaaatactagatgaatactctgttcaatctgtttttcgatttcattactggcatcctgaagaacatcggtaacatcgcagcgtacagcacctgcgacttcatcatggatgaagtagaggtcccgaaagagctgacccagctgcatgaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746200_sequence/1
type: Sequence
persistent identity: BBa I746200 sequence
display id: BBa_I746200_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacaagaagattcattccctggccttgttggtcaatctggggatttatggggtagcgcaggcacaagagccgaccgatactcctgtttcacatgacgatactattgtcgttaccgccgccgagcagaacttacaggcgcctggcgtttcgaccatcaccgcagatgaaatccgcaaaaacccggttgcccgcgatgtgtcgaagatcatccgtaccatgccaggcgttaacctgaccggtaactccaccagtggtcagcgtgggaataaccgacagattgatattcgcggtatgggtccggaaaacacgctgattttgattgacggcaagccggtaagcagccgtaactcggtgcgtcagggctggcgtggcgagcgcgatacccgtggtgatacttcctgggtgccacctgaaatgattgaacgtattgaagttctgcgtggtccggcagctgcgcgttatggcaacggcgcggcgggcggcgtggttaacatcattaccaaaaaaggcagcggcgagtggcacggctcctgggacgcatatttcaatgcgccagaacataaagaggaaggtgccaccaaacgcactaactttagcctgaccggtccgctgggcgacgagttcagcttccgtttgtatggcaacctcgacaaaacccaggctgacgcgtgggatatcaaccagggccatcagtccgcgcgtgccggaacgtatgccacgacgttaccagccgggcgcgaaggggtaatcaacaaagatattaatggcgtggtgcgctgggatttcgcgccattgcaatcgctggaactggaagcaggttacagccgccagggtaacctgtatgcgggcgacacccagaataccaactccgattcctatacccgctcgaaatatggcgatgaaaccaaccgtctgtatcgccagaactacgcgctgacctggaacggtggctgggataacggcgtgaccaccagcaactgggtgcagtacgaacacacccgtaactcgcgtattccggaaggtctggcgggcggtaccgaagggaaatttaacgaaaaagcgacacaggatttcgtcgatatcgatcttgatgacgtgatgctgcacagcgaagttaacctgccgattgatttcctcgttaaccagacgctgacgctgggtacggagtggaatcagcaacggatgaaggacttaagttccaacacccaggcactgaccggaacgaataccggtggcgctattgatggcgtgagtaccaccgaccgtagcccgtattcaaaagcagaaattttctcgctgtttgccgaaaacaacatggagctgactgacagcaccatcgtaacgccggggctgcgtttcgatcatcacagtattgtcggcaataactggagcccggcgctgaacatatcgcaaggtttaggcgatgacttcacgctgaaaatgggcatcgcccgtgcttataaagcgccgagcctgtaccagactaacccgaactacattctctacagtaaaggtcagggttgctatgccagcgcgggcggctgctatctgcaaggtaacgatgacctgaaagcagaaaccagcatcaacaaagagattggtctggagttcaaacgcgacgggtggctggcgggcgtcacctggttccgtaacgattatcgcaataagattgaagcgggctatgtggctgtagggcaaaattgtgaaacagcggtggttgaaggtctggaaggatcgttaaacgtaccggttagcgaaacggtgatgtggaccaataacatcacttatatgctgaagagtgaaaacaaaaccacgggcgaccgtttgtcgatcatcccggagtatacgttgaactcaacgctgagctggcaggcacgggaagatttgtcgatgcaaacgaccttcacctggtacggcaagcagcagccgaagaagtacaactataaaggtcagccagcggttggaccggaaaccaaagaaattagtccttacagcat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23115_sequence/1
type: Sequence
persistent identity: BBa J23115 sequence
display id: BBa_J23115_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttatagctagctcagcccttggtacaatgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z