##gff-version 3
##sequence-region BBa_I746220 1 5504
BBa_I746220	.	terminator	2962	3041	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I746220	.	engineered_region	2962	3041	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I746220	.	stem_loop	2973	3016	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I746220	.	promoter	3099	3133	.	+	0	ID=BBa_J23111;Name=BBa_J23111
BBa_I746220	.	terminator	3050	3090	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I746220	.	engineered_region	3050	3090	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I746220	.	polyA_site	3077	3090	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I746220	.	stop_codon	3083	3083	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I746220	.	stem_loop	3057	3076	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I746220	.	terminator	5464	5504	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I746220	.	engineered_region	5464	5504	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I746220	.	polyA_site	5491	5504	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I746220	.	stop_codon	5497	5497	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I746220	.	stem_loop	5471	5490	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I746220	.	CDS	3160	5367	.	+	0	ID=BBa_I746200;Name=BBa_I746200
BBa_I746220	.	promoter	2109	2204	.	+	0	ID=BBa_I746104;Name=BBa_I746104
BBa_I746220	.	ribosome_entry_site	3142	3153	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I746220	.	conserved_region	3146	3149	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I746220	.	engineered_region	44	1963	.	+	0	ID=BBa_I746100;Name=BBa_I746100
BBa_I746220	.	CDS	62	1309	.	+	0	ID=annotation1951377;Name=agrC coding sequence;Parent=BBa_I746100
BBa_I746220	.	ribosome_entry_site	1316	1327	.	+	0	ID=annotation1951375;Name=B0034-sequence RBS;Parent=BBa_I746100
BBa_I746220	.	CDS	1334	1963	.	+	0	ID=annotation1951378;Name=agrA coding sequence;Parent=BBa_I746100
BBa_I746220	.	ribosome_entry_site	44	55	.	+	0	ID=annotation1951374;Name=B0034-sequence RBS;Parent=BBa_I746100
BBa_I746220	.	sequence_feature	1310	1315	.	+	0	ID=annotation1951376;Name=BamHI site;Parent=BBa_I746100
BBa_I746220	.	terminator	2060	2100	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I746220	.	engineered_region	2060	2100	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I746220	.	polyA_site	2087	2100	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I746220	.	stop_codon	2093	2093	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I746220	.	stem_loop	2067	2086	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I746220	.	terminator	1972	2051	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I746220	.	engineered_region	1972	2051	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I746220	.	stem_loop	1983	2026	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I746220	.	terminator	5376	5455	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I746220	.	engineered_region	5376	5455	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I746220	.	stem_loop	5387	5430	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I746220	.	promoter	1	35	.	+	0	ID=BBa_J23107;Name=BBa_J23107
BBa_I746220	.	CDS	2234	2953	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_I746220	.	CDS	2234	2953	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I746220	.	ribosome_entry_site	2213	2227	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I746220	.	engineered_region	2213	2227	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I746220	.	ribosome_entry_site	2213	2227	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I746220	.	ribosome_entry_site	2220	2224	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
>BBa_I746220
tttacggctagctcagccctaggtattatgctagctactagagaaagaggagaaatactagatgattctgatgttcacc
attccagcaattattagcggtattaagtacagcaagctggattatttcttcatcatcgtaatctctaccctgtccctgt
tcctgttcaaaatgttcgattccgcgagcctgattatcctgacttccttcatcatcatcatgtatttcgtaaaaatcaa
atggtatagcatcctgctgatcatgacctcccagattatcctgtactgtgccaactacatgtatatcgttatctacgcg
tacatcacgaaaatctccgattctatcttcgttatctttccgtctttcttcgtggtgtacgttacgatctctattctgt
tttcctatattatcaatcgcgttctgaaaaagatctctactccgtacctgattctgaacaaaggttttctgatcgtcat
ctccactatcctgctgctgaccttctctctgttcttcttttactctcagatcaactccgatgaggcaaaagtcatccgt
cagtacagcttcatctttatcggtattactatcttcctgagcattctgactttcgttatcagccagttcctgctgaaag
aaatgaagtacaagcgcaaccaagaagaaatcgagacttactacgagtacaccctgaagattgaagcgatcaacaacga
aatgcgtaaatttcgtcacgactacgttaacattctgaccaccctgagcgaatacatccgtgaagacgacatgcctggc
ctgcgtgattatttcaacaaaaacattgtgccgatgaaagataacctgcaaatgaacgctattaagctgaacggtatcg
aaaacctgaaagtacgtgagatcaagggtctgattaccgctaaaatcctgcgtgcgcaggaaatgaacatcccaattag
catcgaaatcccagacgaagtttctagcattaacctgaacatgattgacctgtctcgtagcatcggcatcatcctggac
aacgctatcgaagcttccactgaaatcgacgacccgattattcgcgttgcttttatcgagagcgaaaactccgtgacct
ttatcgtgatgaacaaatgcgcggacgatatcccgcgtattcacgaactgttccaggaatctttcagcaccaagggtga
aggtcgcggcctgggcctgtccaccctgaaagagatcgcggataacgccgacaacgttctgctggacactattatcgaa
aacggtttcttcattcagaaagtcgaaatcatcaataactaataaggatccaaagaggagaaatactagatggaaatcg
cactggctacggacaatccatatgaggttctggaacaggctaaaaacatgaacgacatcggctgctatttcctggatat
tcagctgagcaccgacatcaacggtatcaaactgggctctgaaatccgtaaacatgatccggttggtaacattattttc
gtgacttctcattctgagctgacctatctgacgttcgtatacaaggttgcagccatggatttcatctttaaagacgatc
cggcagagctgcgtacgcgcatcatcgactgtctggaaactgcgcacacgcgcctgcaactgctgtccaaagataactc
cgtggaaaccattgaactgaaacgtggcagcaacagcgtttacgttcagtacgatgatatcatgttcttcgaaagctcc
actaagtcccaccgtctgatcgcgcatctggataatcgtcagatcgagttctatggcaacctgaaagagctgagccagc
tggacgatcgcttcttccgttgtcataactccttcgttgttaaccgtcacaacatcgaatccatcgactccaaagagcg
tattgtatacttcaagaacaaagaacactgttatgcgtctgtacgcaatgtcaagaaaatctaataatactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagattaaatacaaattacatttaacag
ttaagtatttatttcctacagttaggcaatataatgataaaagattgtactaaatcgtataatgacagtgatactagag
attaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatg
gtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatt
tatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcg
agatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatat
ttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagtt
aaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatac
atcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaac
tagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacaca
atctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattaca
catggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttatatactagagttgacggctagctcagtcctaggtatagtgctagctactagagaaagaggagaaatactaga
tgaacaagaagattcattccctggccttgttggtcaatctggggatttatggggtagcgcaggcacaagagccgaccga
tactcctgtttcacatgacgatactattgtcgttaccgccgccgagcagaacttacaggcgcctggcgtttcgaccatc
accgcagatgaaatccgcaaaaacccggttgcccgcgatgtgtcgaagatcatccgtaccatgccaggcgttaacctga
ccggtaactccaccagtggtcagcgtgggaataaccgacagattgatattcgcggtatgggtccggaaaacacgctgat
tttgattgacggcaagccggtaagcagccgtaactcggtgcgtcagggctggcgtggcgagcgcgatacccgtggtgat
acttcctgggtgccacctgaaatgattgaacgtattgaagttctgcgtggtccggcagctgcgcgttatggcaacggcg
cggcgggcggcgtggttaacatcattaccaaaaaaggcagcggcgagtggcacggctcctgggacgcatatttcaatgc
gccagaacataaagaggaaggtgccaccaaacgcactaactttagcctgaccggtccgctgggcgacgagttcagcttc
cgtttgtatggcaacctcgacaaaacccaggctgacgcgtgggatatcaaccagggccatcagtccgcgcgtgccggaa
cgtatgccacgacgttaccagccgggcgcgaaggggtaatcaacaaagatattaatggcgtggtgcgctgggatttcgc
gccattgcaatcgctggaactggaagcaggttacagccgccagggtaacctgtatgcgggcgacacccagaataccaac
tccgattcctatacccgctcgaaatatggcgatgaaaccaaccgtctgtatcgccagaactacgcgctgacctggaacg
gtggctgggataacggcgtgaccaccagcaactgggtgcagtacgaacacacccgtaactcgcgtattccggaaggtct
ggcgggcggtaccgaagggaaatttaacgaaaaagcgacacaggatttcgtcgatatcgatcttgatgacgtgatgctg
cacagcgaagttaacctgccgattgatttcctcgttaaccagacgctgacgctgggtacggagtggaatcagcaacgga
tgaaggacttaagttccaacacccaggcactgaccggaacgaataccggtggcgctattgatggcgtgagtaccaccga
ccgtagcccgtattcaaaagcagaaattttctcgctgtttgccgaaaacaacatggagctgactgacagcaccatcgta
acgccggggctgcgtttcgatcatcacagtattgtcggcaataactggagcccggcgctgaacatatcgcaaggtttag
gcgatgacttcacgctgaaaatgggcatcgcccgtgcttataaagcgccgagcctgtaccagactaacccgaactacat
tctctacagtaaaggtcagggttgctatgccagcgcgggcggctgctatctgcaaggtaacgatgacctgaaagcagaa
accagcatcaacaaagagattggtctggagttcaaacgcgacgggtggctggcgggcgtcacctggttccgtaacgatt
atcgcaataagattgaagcgggctatgtggctgtagggcaaaattgtgaaacagcggtggttgaaggtctggaaggatc
gttaaacgtaccggttagcgaaacggtgatgtggaccaataacatcacttatatgctgaagagtgaaaacaaaaccacg
ggcgaccgtttgtcgatcatcccggagtatacgttgaactcaacgctgagctggcaggcacgggaagatttgtcgatgc
aaacgaccttcacctggtacggcaagcagcagccgaagaagtacaactataaaggtcagccagcggttggaccggaaac
caaagaaattagtccttacagcattgttggcctgagcgcgacctgggatgtgacgaagaatgtcagtctgaccggcggc
gtggacaatctgttcgacaaacgtttgtggcgtgcgggtaatgcccagaccacgggcgatttggcaggggccaactata
tcgccggtgccggggcgtatacctataacgagccgggacgtacgtggtatatgagcgtaaacacccacttctgatacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata