LOCUS       BBa_I746314             2324 bp    DNA     linear       25-Nov-2024
DEFINITION  arabinose induced expression of PSP3 pag and mRFP
ACCESSION   BBa_I746314
VERSION     BBa_I746314.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1219..1230
                     /label=B0034
     misc_feature    1237..1237
                     /label=PSP3 pag
     CDS             1237..1455
                     /label=PSP3 pag
     misc_feature    1219..1455
                     /label=BBa_I746351
     misc_feature    1..1455
                     /label=BBa_I746308
     misc_feature    1468..1471
                     /label=conserved
     RBS             1464..1475
                     /label=BBa_B0034
     CDS             1482..2156
                     /label=mrfp1
     misc_feature    2163..2187
                     /label=Help:Barcodes
     CDS             1482..2162
                     /label=BBa_E1010
     stem_loop       2207..2250
                     /label=stem_loop
     misc_feature    2196..2275
                     /label=BBa_B0010
     terminator      2196..2275
                     /label=BBa_B0010
     stem_loop       2291..2310
                     /label=T7 TE
     misc_feature    2317..2317
                     /label=stop
     polyA_site      2311..2324
                     /label=polya
     misc_feature    2284..2324
                     /label=BBa_B0012
     terminator      2284..2324
                     /label=BBa_B0012
     misc_feature    1464..2324
                     /label=BBa_I13507
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga tgcactgccc gttatgccaa
     1261 aacgctgcac atgctcgcac tagccggtac cttagcaccg aaacgaaaga acgttatcac
     1321 cagtgccaaa acataaattg cggatgtaca tttatcactt ttgagacact atcaagattc
     1381 attgtgaaac cggggactgt tgatcctgct ccgccccacc ccatcagaaa ccaacaacag
     1441 caactttggc tttgatacta gagaaagagg agaaatacta gatggcttcc tccgaagacg
     1501 ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt
     1561 tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga
     1621 aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt
     1681 acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct
     1741 tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg
     1801 ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta
     1861 ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca
     1921 ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac
     1981 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg
     2041 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
     2101 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat
     2161 aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc
     2221 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     2281 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//