LOCUS       BBa_I746315             2351 bp    DNA     linear       22-Dec-2024
DEFINITION  arabinose induced expression of phiR73 delta and mRFP
ACCESSION   BBa_I746315
VERSION     BBa_I746315.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1219..1230
                     /label=B0034
     CDS             1240..1482
                     /label=phiR73 delta
     misc_feature    1240..1240
                     /label=phiR73 delta
     misc_feature    1320..1320
                     /label=silent mutation to remove PstI site
     misc_feature    1219..1482
                     /label=BBa_I746352
     misc_feature    1..1482
                     /label=BBa_I746309
     misc_feature    1495..1498
                     /label=conserved
     RBS             1491..1502
                     /label=BBa_B0034
     CDS             1509..2183
                     /label=mrfp1
     misc_feature    2190..2214
                     /label=Help:Barcodes
     CDS             1509..2189
                     /label=BBa_E1010
     stem_loop       2234..2277
                     /label=stem_loop
     misc_feature    2223..2302
                     /label=BBa_B0010
     terminator      2223..2302
                     /label=BBa_B0010
     stem_loop       2318..2337
                     /label=T7 TE
     misc_feature    2344..2344
                     /label=stop
     polyA_site      2338..2351
                     /label=polya
     misc_feature    2311..2351
                     /label=BBa_B0012
     terminator      2311..2351
                     /label=BBa_B0012
     misc_feature    1491..2351
                     /label=BBa_I13507
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga tgcgctgccc tttctgtcgt
     1261 cattcagcgc atacccgcac cagccggtat gtgagtgaca atgtcaaaga aagttatctc
     1321 cagtgccaga atatttactg ttcggcgaca tttaaaacgc atgagtcaat ttgtgccgtg
     1381 attcgttctc cggtcacgga ggaaaaacca gcaccggcaa gcacagcacc ggctgttgtc
     1441 cgaaaagtta aaggctgtta cagctcacca ttcaaccatt aatactagag aaagaggaga
     1501 aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta
     1561 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt
     1621 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt
     1681 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg
     1741 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga
     1801 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt
     1861 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga
     1921 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga
     1981 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta
     2041 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca
     2101 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg
     2161 aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag
     2221 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     2281 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     2341 ctgcgtttat a
//