LOCUS BBa_I746370 3306 bp DNA linear 22-Dec-2024 DEFINITION P2 ogr inducing PF promoter ACCESSION BBa_I746370 VERSION BBa_I746370.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1219..1230 /label=B0034 misc_feature 1237..1237 /label=P2 ogr CDS 1237..1455 /label=P2 ogr misc_feature 1219..1455 /label=BBa_I746350 misc_feature 1..1455 /label=BBa_I746307 misc_feature 1468..1471 /label=conserved RBS 1464..1475 /label=BBa_B0034 CDS 1482..2156 /label=mrfp1 misc_feature 2163..2187 /label=Help:Barcodes CDS 1482..2162 /label=BBa_E1010 stem_loop 2207..2250 /label=stem_loop misc_feature 2196..2275 /label=BBa_B0010 terminator 2196..2275 /label=BBa_B0010 stem_loop 2291..2310 /label=T7 TE misc_feature 2317..2317 /label=stop polyA_site 2311..2324 /label=polya misc_feature 2284..2324 /label=BBa_B0012 terminator 2284..2324 /label=BBa_B0012 misc_feature 1464..2324 /label=BBa_I13507 misc_feature 1..2324 /label=BBa_I746313 promoter 2333..2423 /label=PF promoter 2333..2423 /label=BBa_I746360 misc_feature 2436..2439 /label=conserved RBS 2432..2443 /label=BBa_B0034 CDS 2450..3169 /label=GFP protein CDS 2450..3169 /label=BBa_E0040 stem_loop 3189..3232 /label=stem_loop misc_feature 3178..3257 /label=BBa_B0010 terminator 3178..3257 /label=BBa_B0010 stem_loop 3273..3292 /label=T7 TE misc_feature 3299..3299 /label=stop polyA_site 3293..3306 /label=polya misc_feature 3266..3306 /label=BBa_B0012 terminator 3266..3306 /label=BBa_B0012 misc_feature 2333..3306 /label=BBa_I746320 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgt ttcattgtcc tttatgccag 1261 catgccgcac atgcgcgtac aagtcgctat atcactgaca cgacaaaaga gcgttatcat 1321 cagtgccaga acgtgaattg cagcgccacg ttcatcactt atgagtcggt acagcgatac 1381 atcgtgaagc cgggagaagt ccacgccgta aggccgcacc cgttgccatc agggcagcaa 1441 attatgtgga tgtaatacta gagaaagagg agaaatacta gatggcttcc tccgaagacg 1501 ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt 1561 tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga 1621 aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt 1681 acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct 1741 tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg 1801 ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta 1861 ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca 1921 ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac 1981 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg 2041 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag 2101 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat 2161 aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc 2221 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 2281 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agttgcccgc 2341 cttttcttta ccggtggttg tgctgtcgat tagccaaccg ggacaaatag cctgacatct 2401 ccggcgcaac tgaaaatacc acttactaga gaaagaggag aaatactaga tgcgtaaagg 2461 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg 2521 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct 2581 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt 2641 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt 2701 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg 2761 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga 2821 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa 2881 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa 2941 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca 3001 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca 3061 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt 3121 aacagctgct gggattacac atggcatgga tgaactatac aaataataat actagagcca 3181 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 3241 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 3301 tttata //