LOCUS BBa_I746382 3307 bp DNA linear 22-Dec-2024 DEFINITION PSP3 pag inducing PP promoter ACCESSION BBa_I746382 VERSION BBa_I746382.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1219..1230 /label=B0034 misc_feature 1237..1237 /label=PSP3 pag CDS 1237..1455 /label=PSP3 pag misc_feature 1219..1455 /label=BBa_I746351 misc_feature 1..1455 /label=BBa_I746308 misc_feature 1468..1471 /label=conserved RBS 1464..1475 /label=BBa_B0034 CDS 1482..2156 /label=mrfp1 misc_feature 2163..2187 /label=Help:Barcodes CDS 1482..2162 /label=BBa_E1010 stem_loop 2207..2250 /label=stem_loop misc_feature 2196..2275 /label=BBa_B0010 terminator 2196..2275 /label=BBa_B0010 stem_loop 2291..2310 /label=T7 TE misc_feature 2317..2317 /label=stop polyA_site 2311..2324 /label=polya misc_feature 2284..2324 /label=BBa_B0012 terminator 2284..2324 /label=BBa_B0012 misc_feature 1464..2324 /label=BBa_I13507 misc_feature 1..2324 /label=BBa_I746314 promoter 2333..2424 /label=PP promoter 2333..2424 /label=BBa_I746362 misc_feature 2437..2440 /label=conserved RBS 2433..2444 /label=BBa_B0034 CDS 2451..3170 /label=GFP protein CDS 2451..3170 /label=BBa_E0040 stem_loop 3190..3233 /label=stem_loop misc_feature 3179..3258 /label=BBa_B0010 terminator 3179..3258 /label=BBa_B0010 stem_loop 3274..3293 /label=T7 TE misc_feature 3300..3300 /label=stop polyA_site 3294..3307 /label=polya misc_feature 3267..3307 /label=BBa_B0012 terminator 3267..3307 /label=BBa_B0012 misc_feature 2333..3307 /label=BBa_I746322 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga tgcactgccc gttatgccaa 1261 aacgctgcac atgctcgcac tagccggtac cttagcaccg aaacgaaaga acgttatcac 1321 cagtgccaaa acataaattg cggatgtaca tttatcactt ttgagacact atcaagattc 1381 attgtgaaac cggggactgt tgatcctgct ccgccccacc ccatcagaaa ccaacaacag 1441 caactttggc tttgatacta gagaaagagg agaaatacta gatggcttcc tccgaagacg 1501 ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt 1561 tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga 1621 aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt 1681 acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct 1741 tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg 1801 ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta 1861 ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca 1921 ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac 1981 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg 2041 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag 2101 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat 2161 aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc 2221 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 2281 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agacggcaag 2341 gctactgagt cgcgccccgc gattcgctaa ggtgctgttg tgtcagtgat aagccatccg 2401 ggactgatgg cggaggatgc gcattactag agaaagagga gaaatactag atgcgtaaag 2461 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg 2521 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc 2581 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt 2641 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt 2701 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg 2761 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg 2821 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca 2881 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta 2941 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac 3001 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac 3061 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg 3121 taacagctgc tgggattaca catggcatgg atgaactata caaataataa tactagagcc 3181 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 3241 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 3301 gtttata //