LOCUS BBa_I746395 3334 bp DNA linear 28-Nov-2024 DEFINITION phiR73 delta inducing PLL promoter ACCESSION BBa_I746395 VERSION BBa_I746395.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1219..1230 /label=B0034 CDS 1240..1482 /label=phiR73 delta misc_feature 1240..1240 /label=phiR73 delta misc_feature 1320..1320 /label=silent mutation to remove PstI site misc_feature 1219..1482 /label=BBa_I746352 misc_feature 1..1482 /label=BBa_I746309 misc_feature 1495..1498 /label=conserved RBS 1491..1502 /label=BBa_B0034 CDS 1509..2183 /label=mrfp1 misc_feature 2190..2214 /label=Help:Barcodes CDS 1509..2189 /label=BBa_E1010 stem_loop 2234..2277 /label=stem_loop misc_feature 2223..2302 /label=BBa_B0010 terminator 2223..2302 /label=BBa_B0010 stem_loop 2318..2337 /label=T7 TE misc_feature 2344..2344 /label=stop polyA_site 2338..2351 /label=polya misc_feature 2311..2351 /label=BBa_B0012 terminator 2311..2351 /label=BBa_B0012 misc_feature 1491..2351 /label=BBa_I13507 misc_feature 1..2351 /label=BBa_I746315 promoter 2360..2451 /label=PLL promoter 2360..2451 /label=BBa_I746365 misc_feature 2464..2467 /label=conserved RBS 2460..2471 /label=BBa_B0034 CDS 2478..3197 /label=GFP protein CDS 2478..3197 /label=BBa_E0040 stem_loop 3217..3260 /label=stem_loop misc_feature 3206..3285 /label=BBa_B0010 terminator 3206..3285 /label=BBa_B0010 stem_loop 3301..3320 /label=T7 TE misc_feature 3327..3327 /label=stop polyA_site 3321..3334 /label=polya misc_feature 3294..3334 /label=BBa_B0012 terminator 3294..3334 /label=BBa_B0012 misc_feature 2460..3334 /label=BBa_I13504 misc_feature 2360..3334 /label=BBa_I746325 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga tgcgctgccc tttctgtcgt 1261 cattcagcgc atacccgcac cagccggtat gtgagtgaca atgtcaaaga aagttatctc 1321 cagtgccaga atatttactg ttcggcgaca tttaaaacgc atgagtcaat ttgtgccgtg 1381 attcgttctc cggtcacgga ggaaaaacca gcaccggcaa gcacagcacc ggctgttgtc 1441 cgaaaagtta aaggctgtta cagctcacca ttcaaccatt aatactagag aaagaggaga 1501 aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc aaagttcgta 1561 tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt 1621 acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt 1681 gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa cacccggctg 1741 acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa cgtgttatga 1801 acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa gacggtgagt 1861 tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg gttatgcaga 1921 aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac ggtgctctga 1981 aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta 2041 aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca 2101 aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg 2161 aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga tcgctactag 2221 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 2281 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 2341 ctgcgtttat atactagagg tggagatgaa caaaaaacac acagccattg taagacagcc 2401 tgaacaaatc ccccctgttg cgtctgctga aaatattcac aaaataaagc gtactagaga 2461 aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc 2521 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag 2581 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg 2641 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc 2701 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg 2761 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg 2821 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa 2881 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag 2941 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa 3001 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt 3061 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga 3121 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg 3181 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 3241 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 3301 ggctcacctt cgggtgggcc tttctgcgtt tata //