LOCUS BBa_I746901 4025 bp DNA linear 25-Nov-2024 DEFINITION test only ACCESSION BBa_I746901 VERSION BBa_I746901.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 misc_feature 1237..3888 /label=T7rnap ATG Start misc_feature 1237..3888 /label=BBa_I716103 stem_loop 3908..3951 /label=stem_loop misc_feature 3897..3976 /label=BBa_B0010 terminator 3897..3976 /label=BBa_B0010 stem_loop 3992..4011 /label=T7 TE misc_feature 4018..4018 /label=stop polyA_site 4012..4025 /label=polya misc_feature 3985..4025 /label=BBa_B0012 terminator 3985..4025 /label=BBa_B0012 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga acacgattaa catcgctaag 1261 aacgacttct ctgacatcga actggctgct atcccgttca acactctggc tgaccattac 1321 ggtgagcgtt tagctcgcga acagttggcc cttgagcatg agtcttacga gatgggtgaa 1381 gcacgcttcc gcaagatgtt tgagcgtcaa cttaaagctg gtgaggttgc ggataacgct 1441 gccgccaagc ctctcatcac taccctactc cctaagatga ttgcacgcat caacgactgg 1501 tttgaggaag tgaaagctaa gcgcggcaag cgcccgacag ccttccagtt cctgcaagaa 1561 atcaagccgg aagccgtagc gtacatcacc attaagacca ctctggcttg cctaaccagt 1621 gctgacaata caaccgttca ggctgtagca agcgcaatcg gtcgggccat tgaggacgag 1681 gctcgcttcg gtcgtatccg tgaccttgaa gctaagcact tcaagaaaaa cgttgaggaa 1741 caactcaaca agcgcgtagg gcacgtctac aagaaagcat ttatgcaagt tgtcgaggct 1801 gacatgctct ctaagggtct actcggtggc gaggcgtggt cttcgtggca taaggaagac 1861 tctattcatg taggagtacg ctgcatcgag atgctcattg agtcaaccgg aatggttagc 1921 ttacaccgcc aaaatgctgg cgtagtaggt caagactctg agactatcga actcgcacct 1981 gaatacgctg aggctatcgc aacccgtgca ggtgcgctgg ctggcatctc tccgatgttc 2041 caaccttgcg tagttcctcc taagccgtgg actggcatta ctggtggtgg ctattgggct 2101 aacggtcgtc gtcctctggc gctggtgcgt actcacagta agaaagcact gatgcgctac 2161 gaagacgttt acatgcctga ggtgtacaaa gcgattaaca ttgcgcaaaa caccgcatgg 2221 aaaatcaaca agaaagtcct agcggtcgcc aacgtaatca ccaagtggaa gcattgtccg 2281 gtcgaggaca tccctgcgat tgagcgtgaa gaactcccga tgaaaccgga agacatcgac 2341 atgaatcctg aggctctcac cgcgtggaaa cgtgctgccg ctgctgtgta ccgcaaggac 2401 aaggctcgca agtctcgccg tatcagcctt gagttcatgc ttgagcaagc caataagttt 2461 gctaaccata aggccatctg gttcccttac aacatggact ggcgcggtcg tgtttacgct 2521 gtgtcaatgt tcaacccgca aggtaacgat atgaccaaag gactgcttac gctggcgaaa 2581 ggtaaaccaa tcggtaagga aggttactac tggctgaaaa tccacggtgc aaactgtgcg 2641 ggtgtcgata aggttccgtt ccctgagcgc atcaagttca ttgaggaaaa ccacgagaac 2701 atcatggctt gcgctaagtc tccactggag aacacttggt gggctgagca agattctccg 2761 ttctgcttcc ttgcgttctg ctttgagtac gctggggtac agcaccacgg cctgagctat 2821 aactgctccc ttccgctggc gtttgacggg tcttgctctg gcatccagca cttctccgcg 2881 atgctccgag atgaggtagg tggtcgcgcg gttaacttgc ttcctagtga aaccgttcag 2941 gacatctacg ggattgttgc taagaaagtc aacgagattc tacaagcaga cgcaatcaat 3001 gggaccgata acgaagtagt taccgtgacc gatgagaaca ctggtgaaat ctctgagaaa 3061 gtcaagctgg gcactaaggc actggctggt caatggctgg cttacggtgt tactcgcagt 3121 gtgactaagc gttcagtcat gacgctggct tacgggtcca aagagttcgg cttccgtcaa 3181 caagtgctgg aagataccat tcagccagct attgattccg gcaagggtct gatgttcact 3241 cagccgaatc aggctgctgg atacatggct aagctgattt gggaatctgt gagcgtgacg 3301 gtggtagctg cggttgaagc aatgaactgg cttaagtctg ctgctaagct gctggctgct 3361 gaggtcaaag ataagaagac tggagagatt cttcgcaagc gttgcgctgt gcattgggta 3421 actcctgatg gtttccctgt gtggcaggaa tacaagaagc ctattcagac gcgcttgaac 3481 ctgatgttcc tcggtcagtt ccgcttacag cctaccatta acaccaacaa agatagcgag 3541 attgatgcac acaaacagga gtctggtatc gctcctaact ttgtacacag ccaagacggt 3601 agccaccttc gtaagactgt agtgtgggca cacgagaagt acggaatcga atcttttgca 3661 ctgattcacg actccttcgg taccattccg gctgacgctg cgaacctgtt caaagcagtg 3721 cgcgaaacta tggttgacac atatgagtct tgtgatgtac tggctgattt ctacgaccag 3781 ttcgctgacc agttgcacga gtctcaattg gacaaaatgc cagcacttcc ggctaaaggt 3841 aacttgaacc tccgtgacat cttagagtcg gacttcgcgt tcgcgtaata ctagagccag 3901 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 3961 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 4021 ttata //