BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_I719005 1 pT7 T7 Promoter 2007-10-23T11:00:00Z 2015-08-31T04:07:53Z --- Released HQ 2013 Just a T7 Promoter false false _128_ 0 2097 9 In stock true None true Imperial 2007 BBa_I746906 1 BBa_I746906 P7 GFP driven by T7 promoter 2008-09-29T11:00:00Z 2015-08-31T04:08:05Z This part was created by standard assembly from construction intermediates: I746911 was put together with I746917:B0015 This is one of the constructs used to characterise a new version of GFP contributed to the registry: P7 GFP (see I746917 part description for source and other information) false false _116_ 0 2122 9 It's complicated false no special considerations true Stefan Milde component1977573 1 BBa_B0012 component1977570 1 BBa_I746917 component1977566 1 BBa_B0034 component1977571 1 BBa_B0010 component1977564 1 BBa_I719005 annotation1977566 1 BBa_B0034 range1977566 1 32 43 annotation1977573 1 BBa_B0012 range1977573 1 866 906 annotation1977570 1 BBa_I746917 range1977570 1 50 769 annotation1977571 1 BBa_B0010 range1977571 1 778 857 annotation1977564 1 BBa_I719005 range1977564 1 1 23 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_I746917 1 BBa_I746917 P7 GFP 2008-09-29T11:00:00Z 2015-08-31T04:08:05Z P7 GFP was created and described by: Fisher et al(2008): "Laboratory evolution of fast-folding green fluorescent protein using secretory pathway quality control", PLoS ONE 3(6) It was biobricked by PCR and standard assembly from DNA kindly provided by Dr Adam C Fisher, Cornell University, New York. Coding region of P7 GFP (Fisher et al(2008): "Laboratory evolution of fast-folding green fluorescent protein using secretory pathway quality control", PLoS ONE 3(6)) P7 GFP carries the following amino acid changes as compared to mut3, the "standard" GFP used in the majority of registry constructs: F64L, G65A, V68L, N105Y, E124V, Y145F Its in vivo properties are the same as those of mut3 GFP (for improved in vivo properties see superfolder GFP: I746916). However, it is more stable in vitro and resists denaturation better than mut3. Additionally it refolds (after denaturation) at a much faster rate than does mut3 GFP - hence it might be useful for in vitro applications. It has been used in the following constructs: Driven by pBAD and T7 promoters: I746904 and I746906 respectively. A 6-his tagged version for purification exists and is driven by pBAD or T7 as well: I746905 and I746907 are the respective part numbers. false false _116_ 0 2122 9 Not in stock false PCR was carried out from plasmid DNA followed by standard assembly into several constructs. false Stefan Milde annotation1977537 1 stop range1977537 1 715 720 annotation1977536 1 start range1977536 1 1 3 annotation1977538 1 P7 GFP coding sequence range1977538 1 1 720 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_I719005_sequence 1 taatacgactcactatagggaga BBa_I746906_sequence 1 taatacgactcactatagggagatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctcgcgtatggtcttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggtactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgtgttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactttaactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctggaattacacatggcatggatgaactatacaaatgatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_I746917_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgacgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctcgcgtatggtcttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggtactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgtgttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactttaactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctggaattacacatggcatggatgaactatacaaatgatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z