LOCUS       BBa_I746907              924 bp    DNA     linear       16-May-2024
DEFINITION  T7 promoter driving 6-his tagged P7 GFP
ACCESSION   BBa_I746907
VERSION     BBa_I746907.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     misc_feature    50..52
                     /label=start
     misc_feature    782..787
                     /label=stop
     CDS             50..763
                     /label=P7 GFP coding region
     misc_feature    764..781
                     /label=6-his tag
     CDS             50..787
                     /label=BBa_I746920
     stem_loop       807..850
                     /label=stem_loop
     misc_feature    796..875
                     /label=BBa_B0010
     terminator      796..875
                     /label=BBa_B0010
     stem_loop       891..910
                     /label=T7 TE
     misc_feature    917..917
                     /label=stop
     polyA_site      911..924
                     /label=polya
     misc_feature    884..924
                     /label=BBa_B0012
     terminator      884..924
                     /label=BBa_B0012
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tgcgtaaagg
       61 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg acgttaatgg
      121 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      181 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactactct
      241 cgcgtatggt cttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
      301 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
      361 gtactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcgt
      421 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
      481 ctttaactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa
      541 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca
      601 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca
      661 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt
      721 aacagctgct ggaattacac atggcatgga tgaactatac aaacatcatc accatcacca
      781 ctaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      841 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      901 cgggtgggcc tttctgcgtt tata
//