LOCUS       BBa_I746914             2111 bp    DNA     linear       23-Nov-2024
DEFINITION  pbad promoter driving 6-his tagged superfolder GFP
ACCESSION   BBa_I746914
VERSION     BBa_I746914.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     misc_feature    1237..1239
                     /label=start
     misc_feature    1969..1974
                     /label=stop
     misc_feature    1951..1968
                     /label=6-his tag
     CDS             1237..1950
                     /label=superfolder GFP coding region
     CDS             1237..1974
                     /label=BBa_I746919
     stem_loop       1994..2037
                     /label=stem_loop
     misc_feature    1983..2062
                     /label=BBa_B0010
     terminator      1983..2062
                     /label=BBa_B0010
     stem_loop       2078..2097
                     /label=T7 TE
     misc_feature    2104..2104
                     /label=stop
     polyA_site      2098..2111
                     /label=polya
     misc_feature    2071..2111
                     /label=BBa_B0012
     terminator      2071..2111
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgc gtaaaggcga agagctgttc
     1261 actggtgtcg tccctattct ggtggaactg gatggtgatg tcaacggtca taagttttcc
     1321 gtgcgtggcg agggtgaagg tgacgcaact aatggtaaac tgacgctgaa gttcatctgt
     1381 actactggta aactgccggt accttggccg actctggtaa cgacgctgac ttatggtgtt
     1441 cagtgctttg ctcgttatcc ggaccatatg aagcagcatg acttcttcaa gtccgccatg
     1501 ccggaaggct atgtgcagga acgcacgatt tcctttaagg atgacggcac gtacaaaacg
     1561 cgtgcggaag tgaaatttga aggcgatacc ctggtaaacc gcattgagct gaaaggcatt
     1621 gactttaaag aagacggcaa tatcctgggc cataagctgg aatacaattt taacagccac
     1681 aatgtttaca tcaccgccga taaacaaaaa aatggcatta aagcgaattt taaaattcgc
     1741 cacaacgtgg aggatggcag cgtgcagctg gctgatcact accagcaaaa cactccaatc
     1801 ggtgatggtc ctgttctgct gccagacaat cactatctga gcacgcaaag cgttctgtct
     1861 aaagatccga acgagaaacg cgatcatatg gttctgctgg agttcgtaac cgcagcgggc
     1921 atcacgcatg gcatggatga actatacaaa catcatcacc atcaccacta ataatactag
     1981 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     2041 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     2101 ctgcgtttat a
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