LOCUS       BBa_I746915              924 bp    DNA     linear       26-Dec-2024
DEFINITION  T7 promoter driving 6-his tagged superfolder GFP
ACCESSION   BBa_I746915
VERSION     BBa_I746915.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     misc_feature    50..52
                     /label=start
     misc_feature    782..787
                     /label=stop
     misc_feature    764..781
                     /label=6-his tag
     CDS             50..763
                     /label=superfolder GFP coding region
     CDS             50..787
                     /label=BBa_I746919
     stem_loop       807..850
                     /label=stem_loop
     misc_feature    796..875
                     /label=BBa_B0010
     terminator      796..875
                     /label=BBa_B0010
     stem_loop       891..910
                     /label=T7 TE
     misc_feature    917..917
                     /label=stop
     polyA_site      911..924
                     /label=polya
     misc_feature    884..924
                     /label=BBa_B0012
     terminator      884..924
                     /label=BBa_B0012
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tgcgtaaagg
       61 cgaagagctg ttcactggtg tcgtccctat tctggtggaa ctggatggtg atgtcaacgg
      121 tcataagttt tccgtgcgtg gcgagggtga aggtgacgca actaatggta aactgacgct
      181 gaagttcatc tgtactactg gtaaactgcc ggtaccttgg ccgactctgg taacgacgct
      241 gacttatggt gttcagtgct ttgctcgtta tccggaccat atgaagcagc atgacttctt
      301 caagtccgcc atgccggaag gctatgtgca ggaacgcacg atttccttta aggatgacgg
      361 cacgtacaaa acgcgtgcgg aagtgaaatt tgaaggcgat accctggtaa accgcattga
      421 gctgaaaggc attgacttta aagaagacgg caatatcctg ggccataagc tggaatacaa
      481 ttttaacagc cacaatgttt acatcaccgc cgataaacaa aaaaatggca ttaaagcgaa
      541 ttttaaaatt cgccacaacg tggaggatgg cagcgtgcag ctggctgatc actaccagca
      601 aaacactcca atcggtgatg gtcctgttct gctgccagac aatcactatc tgagcacgca
      661 aagcgttctg tctaaagatc cgaacgagaa acgcgatcat atggttctgc tggagttcgt
      721 aaccgcagcg ggcatcacgc atggcatgga tgaactatac aaacatcatc accatcacca
      781 ctaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      841 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      901 cgggtgggcc tttctgcgtt tata
//