BBa_I750004
type: ComponentDefinition
persistent identity: BBa I750004
display id: BBa_I750004
version: 1
was generated by: iGEM to SBOL conversion
description: red light receptor GFP test harness
created: 2007-06-29T11:00:00Z
modified: 2015-08-31T04:08:05Z
mutable provenance: All parts are from the standard library.
owned by: myers
owned by: james
mutable description: BBa_M30109 used as the receptor to phosphorylate endogenous OmpR OmpR then binds to OmpC promotor (BBa_R0084) to drive GFP expression using Pops GFP converter BBa_E0241. BBa_E0241 Ribosome binding site : BBa_B0032
discontinued: false
dominant: false
group u list: _132_
m user id: 0
owner id: 1721
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: abcd
star: false
creator: Phillip Dodson
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_E0241
component: BBa_R0084
component: BBa_M30109
sequence annotation: BBa_M30109
sequence annotation: BBa_R0084
sequence annotation: BBa_E0241
sequence: https://synbiohub.org/public/igem/BBa_I750004_sequence/1

BBa_E0241
type: Component
persistent identity: component2313392
display id: component2313392
version: 1
top level: BBa_I750004
definition: BBa_E0241
access: public

BBa_R0084
type: Component
persistent identity: component2313386
display id: component2313386
version: 1
top level: BBa_I750004
definition: OmpR
access: public

BBa_M30109
type: Component
persistent identity: component2313380
display id: component2313380
version: 1
top level: BBa_I750004
definition: BBa_M30109
access: public

BBa_M30109
type: SequenceAnnotation
persistent identity: annotation2313380
display id: annotation2313380
version: 1
top level: BBa_I750004
location: https://synbiohub.org/public/igem/BBa_I750004/annotation2313380/range2313380/1
component: BBa_M30109

https://synbiohub.org/public/igem/BBa_I750004/annotation2313380/range2313380/1
type: Range
persistent identity: range2313380
display id: range2313380
version: 1
top level: BBa_I750004
direction: direction/reverse
start: 1
end: 4333
orientation: inline

BBa_R0084
type: SequenceAnnotation
persistent identity: annotation2313386
display id: annotation2313386
version: 1
top level: BBa_I750004
location: https://synbiohub.org/public/igem/BBa_I750004/annotation2313386/range2313386/1
component: BBa_R0084

https://synbiohub.org/public/igem/BBa_I750004/annotation2313386/range2313386/1
type: Range
persistent identity: range2313386
display id: range2313386
version: 1
top level: BBa_I750004
direction: direction/reverse
start: 4342
end: 4449
orientation: inline

BBa_E0241
type: SequenceAnnotation
persistent identity: annotation2313392
display id: annotation2313392
version: 1
top level: BBa_I750004
location: https://synbiohub.org/public/igem/BBa_I750004/annotation2313392/range2313392/1
component: BBa_E0241

https://synbiohub.org/public/igem/BBa_I750004/annotation2313392/range2313392/1
type: Range
persistent identity: range2313392
display id: range2313392
version: 1
top level: BBa_I750004
direction: direction/reverse
start: 4458
end: 5252
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0084
display id: BBa_R0084
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000913 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompF. Phosphorylated OmpR binds to the three operator sites and activates transcription.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter includes three OmpR binding sites: F1, F2, and F3. The promoter starts at -107 and goes up to the transcriptional start site at +1. A distant fourth binding site, F4, has been deleted. It is involved in negative regulation of ompF (Head, Tardy and Kenney, 1998), so its deletion is intended to make the promoter solely activating. The efficacy of this promoter versus the ompC upstream region (BBa_R0082) or the truncated ompC upstream region (BBa_R0083) is currently unknown.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: F1 OmpR
sequence annotation: F2 OmpR
sequence annotation: F3 OmpR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0084_sequence/1

F1 OmpR
type: SequenceAnnotation
persistent identity: annotation301308
display id: annotation301308
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301308/range301308/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0084/annotation301308/range301308/1
type: Range
persistent identity: range301308
display id: range301308
version: 1
top level: OmpR
direction: direction/forward
start: 11
end: 28
orientation: inline

F2 OmpR
type: SequenceAnnotation
persistent identity: annotation301309
display id: annotation301309
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301309/range301309/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0084/annotation301309/range301309/1
type: Range
persistent identity: range301309
display id: range301309
version: 1
top level: OmpR
direction: direction/forward
start: 31
end: 48
orientation: inline

F3 OmpR
type: SequenceAnnotation
persistent identity: annotation301313
display id: annotation301313
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301313/range301313/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0084/annotation301313/range301313/1
type: Range
persistent identity: range301313
display id: range301313
version: 1
top level: OmpR
direction: direction/forward
start: 51
end: 68
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301317
display id: annotation301317
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301317/range301317/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0084/annotation301317/range301317/1
type: Range
persistent identity: range301317
display id: range301317
version: 1
top level: OmpR
direction: direction/forward
start: 73
end: 78
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301318
display id: annotation301318
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0084/annotation301318/range301318/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0084/annotation301318/range301318/1
type: Range
persistent identity: range301318
display id: range301318
version: 1
top level: OmpR
direction: direction/forward
start: 88
end: 93
orientation: inline

BBa_M30109
type: ComponentDefinition
persistent identity: BBa M30109
display id: BBa_M30109
version: 1
was generated by: iGEM to SBOL conversion
description: Light responsive system, dual regulation
created: 2005-11-14T12:00:00Z
modified: 2015-05-08T01:13:59Z
owned by: james
owned by: myers
mutable description: Pbad regulated phycobilins (ho1 and pcyA) followed by Ptet regulated light sensor (Cph8). This should allow repressed Pbad to give low levels of accessory proteins and derepressed Ptet to crank out lots of light sensor protein
discontinued: false
dominant: true
experience: experience/None
group u list: _45_1_
m user id: 0
owner id: 314
owning group id: 1
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: natalie kuldell
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_R0040
component: BBa_B0012
component: BBa_B0010
component: BBa_I13453
component: BBa_I15010
component: BBa_I15009
component: BBa_I15008
sequence annotation: BBa_I13453
sequence annotation: BBa_B0034
sequence annotation: BBa_I15008
sequence annotation: BBa_I15009
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_I15010
sequence: https://synbiohub.org/public/igem/BBa_M30109_sequence/1

BBa_B0034
type: Component
persistent identity: component1747795
display id: component1747795
version: 1
top level: BBa_M30109
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component1747787
display id: component1747787
version: 1
top level: BBa_M30109
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component1747772
display id: component1747772
version: 1
top level: BBa_M30109
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1747762
display id: component1747762
version: 1
top level: BBa_M30109
definition: BBa_B0010
access: public

BBa_I13453
type: Component
persistent identity: component1747737
display id: component1747737
version: 1
top level: BBa_M30109
definition: BBa_I13453
access: public

BBa_B0012
type: Component
persistent identity: component1747815
display id: component1747815
version: 1
top level: BBa_M30109
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1747805
display id: component1747805
version: 1
top level: BBa_M30109
definition: BBa_B0010
access: public

BBa_I15010
type: Component
persistent identity: component1747799
display id: component1747799
version: 1
top level: BBa_M30109
definition: BBa_I15010
access: public

BBa_I15009
type: Component
persistent identity: component1747756
display id: component1747756
version: 1
top level: BBa_M30109
definition: BBa_I15009
access: public

BBa_B0034
type: Component
persistent identity: component1747752
display id: component1747752
version: 1
top level: BBa_M30109
definition: BBa_B0034
access: public

BBa_I15008
type: Component
persistent identity: component1747746
display id: component1747746
version: 1
top level: BBa_M30109
definition: BBa_I15008
access: public

BBa_B0034
type: Component
persistent identity: component1747742
display id: component1747742
version: 1
top level: BBa_M30109
definition: BBa_B0034
access: public

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation1747737
display id: annotation1747737
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747737/range1747737/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_M30109/annotation1747737/range1747737/1
type: Range
persistent identity: range1747737
display id: range1747737
version: 1
top level: BBa_M30109
direction: direction/forward
start: 1
end: 130
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1747742
display id: annotation1747742
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747742/range1747742/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M30109/annotation1747742/range1747742/1
type: Range
persistent identity: range1747742
display id: range1747742
version: 1
top level: BBa_M30109
direction: direction/forward
start: 139
end: 150
orientation: inline

BBa_I15008
type: SequenceAnnotation
persistent identity: annotation1747746
display id: annotation1747746
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747746/range1747746/1
component: BBa_I15008

https://synbiohub.org/public/igem/BBa_M30109/annotation1747746/range1747746/1
type: Range
persistent identity: range1747746
display id: range1747746
version: 1
top level: BBa_M30109
direction: direction/forward
start: 157
end: 882
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1747752
display id: annotation1747752
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747752/range1747752/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M30109/annotation1747752/range1747752/1
type: Range
persistent identity: range1747752
display id: range1747752
version: 1
top level: BBa_M30109
direction: direction/forward
start: 916
end: 927
orientation: inline

BBa_I15009
type: SequenceAnnotation
persistent identity: annotation1747756
display id: annotation1747756
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747756/range1747756/1
component: BBa_I15009

https://synbiohub.org/public/igem/BBa_M30109/annotation1747756/range1747756/1
type: Range
persistent identity: range1747756
display id: range1747756
version: 1
top level: BBa_M30109
direction: direction/forward
start: 934
end: 1683
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1747762
display id: annotation1747762
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747762/range1747762/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M30109/annotation1747762/range1747762/1
type: Range
persistent identity: range1747762
display id: range1747762
version: 1
top level: BBa_M30109
direction: direction/forward
start: 1717
end: 1796
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1747772
display id: annotation1747772
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747772/range1747772/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M30109/annotation1747772/range1747772/1
type: Range
persistent identity: range1747772
display id: range1747772
version: 1
top level: BBa_M30109
direction: direction/forward
start: 1805
end: 1845
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1747787
display id: annotation1747787
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747787/range1747787/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_M30109/annotation1747787/range1747787/1
type: Range
persistent identity: range1747787
display id: range1747787
version: 1
top level: BBa_M30109
direction: direction/forward
start: 1854
end: 1907
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1747795
display id: annotation1747795
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747795/range1747795/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M30109/annotation1747795/range1747795/1
type: Range
persistent identity: range1747795
display id: range1747795
version: 1
top level: BBa_M30109
direction: direction/forward
start: 1916
end: 1927
orientation: inline

BBa_I15010
type: SequenceAnnotation
persistent identity: annotation1747799
display id: annotation1747799
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747799/range1747799/1
component: BBa_I15010

https://synbiohub.org/public/igem/BBa_M30109/annotation1747799/range1747799/1
type: Range
persistent identity: range1747799
display id: range1747799
version: 1
top level: BBa_M30109
direction: direction/forward
start: 1934
end: 4171
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1747805
display id: annotation1747805
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747805/range1747805/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M30109/annotation1747805/range1747805/1
type: Range
persistent identity: range1747805
display id: range1747805
version: 1
top level: BBa_M30109
direction: direction/forward
start: 4205
end: 4284
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1747815
display id: annotation1747815
version: 1
top level: BBa_M30109
location: https://synbiohub.org/public/igem/BBa_M30109/annotation1747815/range1747815/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M30109/annotation1747815/range1747815/1
type: Range
persistent identity: range1747815
display id: range1747815
version: 1
top level: BBa_M30109
direction: direction/forward
start: 4293
end: 4333
orientation: inline

BBa_E0241
type: ComponentDefinition
persistent identity: BBa E0241
display id: BBa_E0241
version: 1
was generated by: iGEM to SBOL conversion
description: PoPS to GFP converter
created: 2005-09-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
mutable description: This part accepts input PoPS and outputs untagged GFP. The part is very similar to E0240 but uses a T7 RNAP transcription terminator rather than an E. coli RNAP transcription terminator. This part will be used in a reporter device for dedicated transcription systems.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 6
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0032
component: BBa_B0016
component: BBa_E0040
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0016
sequence: https://synbiohub.org/public/igem/BBa_E0241_sequence/1

BBa_B0032
type: Component
persistent identity: component1707881
display id: component1707881
version: 1
top level: BBa_E0241
definition: BBa_B0032
access: public

BBa_B0016
type: Component
persistent identity: component1707886
display id: component1707886
version: 1
top level: BBa_E0241
definition: BBa_B0016
access: public

BBa_E0040
type: Component
persistent identity: component1707884
display id: component1707884
version: 1
top level: BBa_E0241
definition: GFP
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1707881
display id: annotation1707881
version: 1
top level: BBa_E0241
location: https://synbiohub.org/public/igem/BBa_E0241/annotation1707881/range1707881/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_E0241/annotation1707881/range1707881/1
type: Range
persistent identity: range1707881
display id: range1707881
version: 1
top level: BBa_E0241
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1707884
display id: annotation1707884
version: 1
top level: BBa_E0241
location: https://synbiohub.org/public/igem/BBa_E0241/annotation1707884/range1707884/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0241/annotation1707884/range1707884/1
type: Range
persistent identity: range1707884
display id: range1707884
version: 1
top level: BBa_E0241
direction: direction/forward
start: 20
end: 739
orientation: inline

BBa_B0016
type: SequenceAnnotation
persistent identity: annotation1707886
display id: annotation1707886
version: 1
top level: BBa_E0241
location: https://synbiohub.org/public/igem/BBa_E0241/annotation1707886/range1707886/1
component: BBa_B0016

https://synbiohub.org/public/igem/BBa_E0241/annotation1707886/range1707886/1
type: Range
persistent identity: range1707886
display id: range1707886
version: 1
top level: BBa_E0241
direction: direction/forward
start: 748
end: 795
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_I15008
type: ComponentDefinition
persistent identity: BBa I15008
display id: BBa_I15008
version: 1
was generated by: iGEM to SBOL conversion
description: heme oxygenase (ho1) from Synechocystis
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: One of two requisite genes required for the biosynthesis of phycocyanobilin from heme.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jeff Tabor
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15008_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214026
display id: annotation2214026
version: 1
top level: BBa_I15008
location: https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1
type: Range
persistent identity: range2214026
display id: range2214026
version: 1
top level: BBa_I15008
direction: direction/forward
start: 727
end: 751
orientation: inline

BBa_I15009
type: ComponentDefinition
persistent identity: BBa I15009
display id: BBa_I15009
version: 1
was generated by: iGEM to SBOL conversion
description: phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Second of two required phycocyanobilin biosynthetic genes.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jeff Tabor
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PcyA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15009_sequence/1

PcyA
type: SequenceAnnotation
persistent identity: annotation1891612
display id: annotation1891612
version: 1
top level: BBa_I15009
location: https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1
type: Range
persistent identity: range1891612
display id: range1891612
version: 1
top level: BBa_I15009
direction: direction/forward
start: 1
end: 750
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214032
display id: annotation2214032
version: 1
top level: BBa_I15009
location: https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1
type: Range
persistent identity: range2214032
display id: range2214032
version: 1
top level: BBa_I15009
direction: direction/forward
start: 751
end: 775
orientation: inline

BBa_I15010
type: ComponentDefinition
persistent identity: BBa I15010
display id: BBa_I15010
version: 1
was generated by: iGEM to SBOL conversion
description: cph8 (Cph1/EnvZ fusion)
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
mutable description: Chimeric Cph1 light receptor/EnvZ protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Silent mutation at base 108 (G-A) to remove PstI site
star: false
creator: Jeff Tabor
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cph8
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15010_sequence/1

Cph8
type: SequenceAnnotation
persistent identity: annotation1891613
display id: annotation1891613
version: 1
top level: BBa_I15010
location: https://synbiohub.org/public/igem/BBa_I15010/annotation1891613/range1891613/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I15010/annotation1891613/range1891613/1
type: Range
persistent identity: range1891613
display id: range1891613
version: 1
top level: BBa_I15010
direction: direction/forward
start: 1
end: 2238
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214031
display id: annotation2214031
version: 1
top level: BBa_I15010
location: https://synbiohub.org/public/igem/BBa_I15010/annotation2214031/range2214031/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I15010/annotation2214031/range2214031/1
type: Range
persistent identity: range2214031
display id: range2214031
version: 1
top level: BBa_I15010
direction: direction/forward
start: 2239
end: 2263
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0016
type: ComponentDefinition
persistent identity: BBa B0016
display id: BBa_B0016
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (T7 RNAP specific, T_Phi)
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Sri Kosuri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: T7 gene 1 specific transcriptional terminator
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Sri Kosuri
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_B0016_sequence/1

https://synbiohub.org/public/igem/BBa_I750004_sequence/1
type: Sequence
persistent identity: BBa I750004 sequence
display id: BBa_I750004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0084_sequence/1
type: Sequence
persistent identity: BBa R0084 sequence
display id: BBa_R0084_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaaacgttagtttgaatggaaagatgcctgca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0241_sequence/1
type: Sequence
persistent identity: BBa E0241 sequence
display id: BBa_E0241_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagctagcataaccccttggggcctctaaacgggtcttgaggggttttttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M30109_sequence/1
type: Sequence
persistent identity: BBa M30109 sequence
display id: BBa_M30109_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15008_sequence/1
type: Sequence
persistent identity: BBa I15008 sequence
display id: BBa_I15008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15009_sequence/1
type: Sequence
persistent identity: BBa I15009 sequence
display id: BBa_I15009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15010_sequence/1
type: Sequence
persistent identity: BBa I15010 sequence
display id: BBa_I15010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0016_sequence/1
type: Sequence
persistent identity: BBa B0016 sequence
display id: BBa_B0016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagcataaccccttggggcctctaaacgggtcttgaggggttttttg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z