##gff-version 3
##sequence-region BBa_I750010 1 590
BBa_I750010	.	promoter	1	108	.	+	0	ID=BBa_R0084;Name=BBa_R0084
BBa_I750010	.	non_covalent_binding_site	31	48	.	+	0	ID=annotation301309;Name=F2 OmpR;Parent=BBa_R0084
BBa_I750010	.	promoter	88	93	.	+	0	ID=annotation301318;Name=-10;Parent=BBa_R0084
BBa_I750010	.	promoter	73	78	.	+	0	ID=annotation301317;Name=-35;Parent=BBa_R0084
BBa_I750010	.	non_covalent_binding_site	51	68	.	+	0	ID=annotation301313;Name=F3 OmpR;Parent=BBa_R0084
BBa_I750010	.	non_covalent_binding_site	11	28	.	+	0	ID=annotation301308;Name=F1 OmpR;Parent=BBa_R0084
BBa_I750010	.	terminator	496	536	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I750010	.	engineered_region	496	536	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I750010	.	stem_loop	503	522	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I750010	.	polyA_site	523	536	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I750010	.	stop_codon	529	529	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I750010	.	engineered_region	135	487	.	+	0	ID=BBa_E0033;Name=BBa_E0033
BBa_I750010	.	sequence_alteration	215	215	.	+	0	ID=annotation308375;Name=T;Parent=BBa_E0033
BBa_I750010	.	CDS	135	482	.	+	0	ID=annotation308320;Name=lacZ alpha;Parent=BBa_E0033
BBa_I750010	.	sequence_alteration	245	245	.	+	0	ID=annotation308388;Name=G;Parent=BBa_E0033
BBa_I750010	.	sequence_alteration	212	212	.	+	0	ID=annotation308387;Name=C;Parent=BBa_E0033
BBa_I750010	.	promoter	160	169	.	+	0	ID=annotation1938939;Name=T3 promoter;Parent=BBa_E0033
BBa_I750010	.	sequence_feature	135	149	.	+	0	ID=annotation1938942;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_I750010	.	promoter	307	325	.	+	0	ID=annotation1938940;Name=T7 promoter;Parent=BBa_E0033
BBa_I750010	.	sequence_feature	333	482	.	+	0	ID=annotation1938941;Name=lacZ gene fragment;Parent=BBa_E0033
BBa_I750010	.	sequence_alteration	221	221	.	+	0	ID=annotation308381;Name=A;Parent=BBa_E0033
BBa_I750010	.	sequence_alteration	242	242	.	+	0	ID=annotation308383;Name=C;Parent=BBa_E0033
BBa_I750010	.	ribosome_entry_site	117	128	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I750010	.	conserved_region	121	124	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I750010	.	terminator	545	590	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I750010	.	stem_loop	557	579	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I750010	.	engineered_region	545	590	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
>BBa_I750010
cttaaattttacttttggttacatattttttctttttgaaaccaaatctttatctttgtagcactttcacggtagcgaa
acgttagtttgaatggaaagatgcctgcatactagagaaagaggagaaatactagatgaccatgattacgccaagcgcg
caattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgg
gctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtga
gtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgc
cttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgca
gcctgaataataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatat
aaaaagccagattattaatccggcttttttattattt