LOCUS       BBa_I750012             2468 bp    DNA     linear       22-Dec-2024
DEFINITION  RBS + ComPalt + ter
ACCESSION   BBa_I750012
VERSION     BBa_I750012.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..2331
                     /label=BBa_I750011
     stem_loop       2351..2394
                     /label=stem_loop
     misc_feature    2340..2419
                     /label=BBa_B0010
     terminator      2340..2419
                     /label=BBa_B0010
     stem_loop       2435..2454
                     /label=T7 TE
     misc_feature    2461..2461
                     /label=stop
     polyA_site      2455..2468
                     /label=polya
     misc_feature    2428..2468
                     /label=BBa_B0012
     terminator      2428..2468
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gaaaaacctg atcaaaaaat tcaccattgc ggtgattgtt
       61 ctgagcattc tgtatatcag ctacaccacc tatattagca tgaacggcat tattatcggc
      121 accaaaatcc acaaaaacga taaaagccag ttcatgatcg aagaaattag cgaaagcagc
      181 tatggccagt ttgtgggcct gcgtcaggcg gatattattc tgaaaatcaa taaagaaaaa
      241 ccgagcgata aacatctgaa atggggctat ctgagccata ttaacagcct ggatattctg
      301 cgtagcggca aaaaaatcca tctgaaagat ttcgatctgg tgaccctgaa ccgtccgtat
      361 agcttttttc tgtttgtgct gccgctgttt ttttatttcc tgagcatcat ctgcatcttc
      421 tacatcctga aagtgaacaa aaaacgtcgt agctttgcgg cgtatattct gattctgctg
      481 ctgctggata ttagcattgc gtatattagc gcgggtggcc cgtttcgtgg ccatattatc
      541 aaccgctata ttaacctgtt tacctttatt agcagcccga ttctgtatct gcaatttatt
      601 cagcgttatc tgggcgaaat tggcaaaacc tttctgaacc gcatcagctt tctgtatatt
      661 atcccgatct ttaacctggg catcgaattt tttcaggatt acctgcaagt ggatattgat
      721 tttctggcca ccctgaacct ggtgagcttt gcgaccctga ccctgtttag ctttagcgcg
      781 atttatctgc acctgaacaa atacaaatat gcggaacaca gctttattct gaaactgctg
      841 attctgacca acaccctgag ctttgcgccg tttctgattt ttttcgtgct gccgattatt
      901 tttaccggca actatatttt tccggccctg gccagcgcga gcctgctggt gctgattccg
      961 tttggcctgg tgtatcagtt tgtggcgaac aaaatgttcg atatcgaatt tattctgggc
     1021 cgtatgcgct attatgcgct gctggccatg attccgaccc tgctgattgt gggcgcgctg
     1081 gtgctgtttg atgtgatgga tatccagatg aatccggtgc gtcagaccgt gttttttttc
     1141 gtggtgatgt ttgcggtgtt ctacttcaaa gaagtgatgg atttcaaatt tcgcctgaaa
     1201 cgcttcagcg aaaaattcaa ctaccaggat agcatcttca agtacaccca gctgatgcgt
     1261 ggcgtgacca gcctgcaaca ggtgtttaaa gaactgaaaa acaccattct ggatgtgctg
     1321 ctggtgagca aagcgtatac ctttgaagtg accccggatc ataaagtgat ctttctggat
     1381 aaacatgaag tgggcccgga ttggaacttt tatcaggaag aatttgaaaa cgttaccagc
     1441 gaaatcggta aaatcatcga agtgaaccag ggctttctga tgaaagtggg cgaacgtggc
     1501 ggcagcagct atgtgctgct gtgcctgagc aacattaaca ccccgcgtct gacccgtgat
     1561 gaaattagct ggctgaaaac cctgagcttc tataccagcg tgagcatgga aaacgtgctg
     1621 catattgaag aactgatgga acacctgaaa gatctgaaac aggaaggcac caatccgatt
     1681 tggctgaaaa aactgatgtt cgcgatcgaa gaaaaacagc gtagcggcct ggcccgtgat
     1741 ctgcatgata gcgtgctgca agatctgatt agcctgaaac gtcagtgcga actgtttctg
     1801 ggcgatttca aaaaagatga taatccgagc cgtgaagaag tgcaggataa actggtgcag
     1861 atgaacgagc agatgagcga tgtgattagc atgacccgtg aaacctgcca tgaactgcgt
     1921 ccgcagctgc tgtatgatct gggcctggtg aaagccctga gcaaactggt ggcgcatgaa
     1981 caggaacgtg ttccgtttca tattcgtctg aacaccggcc gttttaccgc gagcctggat
     2041 ctggatagcc agctgaacct gtatcgtatc atccaggaat ttctgagcaa cgcggtgaaa
     2101 catagccagg cgaccgatgt gctgattatg ctgatcagta tccagaacaa aatcgtgctg
     2161 cactacgaag atgatggcgt gggctttgat caggaaaaaa acaccgaaca tagcatgagc
     2221 atgggcctga gcggcattaa agaacgtgtg cgtgcgctgg atggccgtct gcgtattgaa
     2281 accagcgaag gcaaaggctt taaagcggat atcgaaatcg aactgtaata atactagagc
     2341 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     2401 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     2461 cgtttata
//