LOCUS BBa_I750017 4649 bp DNA linear 20-Apr-2025 DEFINITION EcoRI-GenR-XbaI on pSB1AK3 backbone ACCESSION BBa_I750017 VERSION BBa_I750017.1 FEATURES Location/Qualifiers promoter 2969..2997 /label=beta-lactamase (ampR) promoter CDS 3645..4178 /label=gentamycin resistance marker misc_feature 3201..3584 /label=R6Kg origin CDS 2067..2927 /label=beta-lactamase ampicillin resistance marker CDS 1097..1912 /label=kanamycin resistance misc_feature 261..875 /label=pMB1 origin misc_feature 1..21 /label=BioBrick suffix misc_feature 3168..3173 /label=EcoRI misc_feature 3624..3631 /label=NotI misc_feature 4643..4648 /label=XbaI primer_bind 3050..3069 /label=Verfication forward (VF2) primer binding site stem_loop 3139..3158 /label=Stem loop stem_loop 3126..3129 /label=End of stem loop stem_loop 22..93 /label=E. coli his operon terminator stem_loop 30..64 /label=Stem loop misc_feature 34..35 /label=Deletion of AC primer_bind 157..176 /label=Verification reverse (VR) primer binding site ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct 61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 961 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 1021 acttggtctg acagctcgag tcccgtcaag tcagcgtaat gctctgccag tgttacaacc 1081 aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca 1141 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact 1201 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc 1261 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat 1321 caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga 1381 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt 1441 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat 1501 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt 1561 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg 1621 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa 1681 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt 1741 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg 1801 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt 1861 tggaatttaa tcgcggcctg gagcaagacg tttcccgttg aatatggctc ataacacccc 1921 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt 1981 gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata aatcgaactt 2041 ttgctgagtt gaaggatcag ctcgagttac caatgcttaa tcagtgaggc acctatctca 2101 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 2161 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 2221 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 2281 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 2341 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 2401 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 2461 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 2521 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 2581 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 2641 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 2701 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 2761 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 2821 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 2881 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 2941 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 3001 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 3061 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag 3121 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttctgattcg 3181 cacgggccca tggctaattc ccatgtcagc cgttaagtgt tcctgtgtca ctcaaaattg 3241 ctttgagagg ctctaagggc ttctcagtgc gttacatccc tggcttgttg tccacaaccg 3301 ttaaacctta aaagctttaa aagccttata tattcttttt tttcttataa aacttaaaac 3361 cttagaggct atttaagttg ctgatttata ttaattttat tgttcaaaca tgagagctta 3421 gtacgtgaaa catgagagct tagtacgtta gccatgagag cttagtacgt tagccatgag 3481 ggtttagttc gttaaacatg agagcttagt acgttaaaca tgagagctta gtacgtgaaa 3541 catgagagct tagtacgtac tatcaacagg ttgaactgct gatcttcaga tcctctacgc 3601 cggacgcatc gtggccggat cttgcggccg ccttgaacga attgttaggt ggcggtactt 3661 gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact 3721 gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc 3781 tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg ctctccggag 3841 actgcgagat catagatata gatctcacta cgcggctgct caaacttggg cagaacgtaa 3901 gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta 3961 ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctacg 4021 tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtcaggg 4081 ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt tttagggcga 4141 ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa 4201 cgcgcttgct gcttggatgc ccgaggcata gactgtacaa aaaaacagtc ataacaagcc 4261 atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg 4321 cgtgagcgca tacgctactt gcattacagt ttacgaaccg aacaggctta tgtcaactgg 4381 gttcgtgggc gcctgagctt atgatgtggt ctgtcctttt acagccagta gtgctcgccg 4441 cagtcgagcg acagggcgaa gccctcgagt gagcgaggaa gcaccaggga acagcactta 4501 tatattctgc ttacacacga tgcctgaaaa aacttccctt ggggttatcc acttatccac 4561 ggggatattt ttataattat tttttttata gtttttagat cttctttttt agagcgcctt 4621 gtaggccttt atccatgctg gttctagag //