##gff-version 3
##sequence-region BBa_I750017 1 4648
BBa_I750017	.	stem_loop	22	93	.	+	0	ID=annotation1975970;Name=E. coli his operon terminator
BBa_I750017	.	sequence_feature	3624	3631	.	+	0	ID=annotation1975965;Name=NotI
BBa_I750017	.	primer_binding_site	157	176	.	+	0	ID=annotation1975973;Name=Verification reverse (VR) primer binding site
BBa_I750017	.	CDS	1097	1912	.	+	0	ID=annotation1952594;Name=kanamycin resistance
BBa_I750017	.	sequence_feature	3168	3173	.	+	0	ID=annotation1975964;Name=EcoRI
BBa_I750017	.	sequence_feature	3201	3584	.	+	0	ID=annotation1952592;Name=R6Kg origin
BBa_I750017	.	stem_loop	3139	3158	.	+	0	ID=annotation1975968;Name=Stem loop
BBa_I750017	.	sequence_alteration	34	35	.	+	0	ID=annotation1975972;Name=Deletion of AC
BBa_I750017	.	CDS	2067	2927	.	+	0	ID=annotation1952593;Name=beta-lactamase ampicillin resistance marker
BBa_I750017	.	stem_loop	3126	3129	.	+	0	ID=annotation1975969;Name=End of stem loop
BBa_I750017	.	CDS	3645	4178	.	+	0	ID=annotation1952591;Name=gentamycin resistance marker
BBa_I750017	.	sequence_feature	1	21	.	+	0	ID=annotation1975963;Name=BioBrick suffix
BBa_I750017	.	stem_loop	30	64	.	+	0	ID=annotation1975971;Name=Stem loop
BBa_I750017	.	sequence_feature	4643	4648	.	+	0	ID=annotation1975966;Name=XbaI
BBa_I750017	.	primer_binding_site	3050	3069	.	+	0	ID=annotation1975967;Name=Verfication forward (VF2) primer binding site
BBa_I750017	.	promoter	2969	2997	.	+	0	ID=annotation1952590;Name=beta-lactamase (ampR) promoter
BBa_I750017	.	sequence_feature	261	875	.	+	0	ID=annotation1975962;Name=pMB1 origin
>BBa_I750017
tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaa
gattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaa
aaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatc
gacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcg
ctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagc
tcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccg
accgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccac
tggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacact
agaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggca
aacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaaga
tcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatca
aaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggt
ctgacagctcgagtcccgtcaagtcagcgtaatgctctgccagtgttacaaccaattaaccaattctgattagaaaaac
tcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgta
atgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaac
atcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatcc
ggtgagaatggcaaaagcttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcac
tcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaatt
acaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattct
tctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgct
tgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacc
tttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgaca
ttatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctggagcaagacgtttccc
gttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttcatgatgatatattttt
atcttgtgcaatgtaacatcagagattttgagacacaacgtggctttgttgaataaatcgaacttttgctgagttgaag
gatcagctcgagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcc
tgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacc
cacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaacttt
atccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgtt
gttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaa
ggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagtt
ggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttct
gtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatac
gggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaag
gatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcacc
agcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatac
tcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtat
ttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatc
atgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatg
atttctggaattctgattcgcacgggcccatggctaattcccatgtcagccgttaagtgttcctgtgtcactcaaaatt
gctttgagaggctctaagggcttctcagtgcgttacatccctggcttgttgtccacaaccgttaaaccttaaaagcttt
aaaagccttatatattcttttttttcttataaaacttaaaaccttagaggctatttaagttgctgatttatattaattt
tattgttcaaacatgagagcttagtacgtgaaacatgagagcttagtacgttagccatgagagcttagtacgttagcca
tgagggtttagttcgttaaacatgagagcttagtacgttaaacatgagagcttagtacgtgaaacatgagagcttagta
cgtactatcaacaggttgaactgctgatcttcagatcctctacgccggacgcatcgtggccggatcttgcggccgcctt
gaacgaattgttaggtggcggtacttgggtcgatatcaaagtgcatcacttcttcccgtatgcccaactttgtatagag
agccactgcgggatcgtcaccgtaatctgcttgcacgtagatcacataagcaccaagcgcgttggcctcatgcttgagg
agattgatgagcgcggtggcaatgccctgcctccggtgctctccggagactgcgagatcatagatatagatctcactac
gcggctgctcaaacttgggcagaacgtaagccgcgagagcgccaacaaccgcttcttggtcgaaggcagcaagcgcgat
gaatgtcttactacggagcaagttcccgaggtaatcggagtccggctgatgttgggagtaggtggctacgtctccgaac
tcacgaccgaaaagatcaagagcagcccgcatggatttgacttggtcagggccgagcctacatgtgcgaatgatgccca
tacttgagccacctaactttgttttagggcgactgccctgctgcgtaacatcgttgctgctccataacatcaaacatcg
acccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtacaaaaaaacagtcataacaagccatgaaa
accgccactgcgccgttaccaccgctgcgttcggtcaaggttctggaccagttgcgtgagcgcatacgctacttgcatt
acagtttacgaaccgaacaggcttatgtcaactgggttcgtgggcgcctgagcttatgatgtggtctgtccttttacag
ccagtagtgctcgccgcagtcgagcgacagggcgaagccctcgagtgagcgaggaagcaccagggaacagcacttatat
attctgcttacacacgatgcctgaaaaaacttcccttggggttatccacttatccacggggatatttttataattattt
tttttatagtttttagatcttcttttttagagcgccttgtaggcctttatccatgctggttctagag