LOCUS BBa_I751111 2964 bp DNA linear 26-Dec-2024 DEFINITION J54140dP + hyprom + cI ACCESSION BBa_I751111 VERSION BBa_I751111.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 158..159 /label=prefix misc_feature 331..331 /label=T misc_feature 649..649 /label=A CDS 158..907 /label=luxR misc_feature 158..913 /label=BBa_C0062 misc_feature 914..938 /label=Help:Barcodes CDS 158..913 /label=BBa_C0062 stem_loop 958..1001 /label=stem_loop misc_feature 947..1026 /label=BBa_B0010 terminator 947..1026 /label=BBa_B0010 stem_loop 1042..1061 /label=T7 TE misc_feature 1068..1068 /label=stop polyA_site 1062..1075 /label=polya misc_feature 1035..1075 /label=BBa_B0012 terminator 1035..1075 /label=BBa_B0012 misc_feature 1084..1088 /label=SalI misc_feature 1098..1102 /label=BamHI misc_feature 1084..1102 /label=BBa_J54033 promoter 1130..1184 /label=plac misc_feature 1130..1136 /label=-35 misc_feature 1152..1158 /label=-10 misc_feature 1137..1151 /label=O1 misc_feature 1159..1184 /label=O1 misc_feature 1111..1129 /label=luxR binding site promoter 1111..1184 /label=BBa_I751502 misc_feature 1193..1218 /label=BBa_I751400 misc_feature 1231..1234 /label=conserved RBS 1227..1238 /label=BBa_B0034 CDS 1248..1955 /label=cI lambda misc_feature 1956..1988 /label=LVA misc_feature 1995..2019 /label=Help:Barcodes CDS 1245..1994 /label=BBa_C0051 misc_feature 1227..2019 /label=BBa_S0103 misc_feature 2028..2051 /label=BBa_I751410 misc_feature 2060..2064 /label=BglII misc_feature 2073..2078 /label=MulI misc_feature 2060..2078 /label=BBa_J54025 RBS 2087..2101 /label=RBS-1\Strong RBS 2094..2098 /label=RBS misc_feature 2087..2101 /label=BBa_B0030 RBS 2087..2101 /label=BBa_B0030 CDS 2108..2827 /label=GFP protein CDS 2108..2827 /label=BBa_E0040 stem_loop 2847..2890 /label=stem_loop misc_feature 2836..2915 /label=BBa_B0010 terminator 2836..2915 /label=BBa_B0010 stem_loop 2931..2950 /label=T7 TE misc_feature 2957..2957 /label=stop polyA_site 2951..2964 /label=polya misc_feature 2924..2964 /label=BBa_B0012 terminator 2924..2964 /label=BBa_B0012 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagatg aaaaacataa atgccgacga 181 cacatacaga ataattaata aaattaaagc ttgtagaagc aataatgata ttaatcaatg 241 cttatctgat atgactaaaa tggtacattg tgaatattat ttactcgcga tcatttatcc 301 tcattctatg gttaaatctg atatttcaat cctagataat taccctaaaa aatggaggca 361 atattatgat gacgctaatt taataaaata tgatcctata gtagattatt ctaactccaa 421 tcattcacca attaattgga atatatttga aaacaatgct gtaaataaaa aatctccaaa 481 tgtaattaaa gaagcgaaaa catcaggtct tatcactggg tttagtttcc ctattcatac 541 ggctaacaat ggcttcggaa tgcttagttt tgcacattca gaaaaagaca actatataga 601 tagtttattt ttacatgcgt gtatgaacat accattaatt gttccttctc tagttgataa 661 ttatcgaaaa ataaatatag caaataataa atcaaacaac gatttaacca aaagagaaaa 721 agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg gatatttcaa aaatattagg 781 ttgcagtgag cgtactgtca ctttccattt aaccaatgcg caaatgaaac tcaatacaac 841 aaaccgctgc caaagtattt ctaaagcaat tttaacagga gcaattgatt gcccatactt 901 taaaaattaa taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata 961 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 1021 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 1081 gaggtcgact gacgactgga tctactagag acctgtagga tcgtacaggt ttacttgtga 1141 gcggataaca atatagtgtg tggaattgtg agcggataac aatttactag agctagtgtc 1201 gactgacgac tggatccata ctagagaaag aggagaaata ctagatgagc acaaaaaaga 1261 aaccattaac acaagagcag cttgaggacg cacgtcgcct taaagcaatt tatgaaaaaa 1321 agaaaaatga acttggctta tcccaggaat ctgtcgcaga caagatgggg atggggcagt 1381 caggcgttgg tgctttattt aatggcatca atgcattaaa tgcttataac gccgcattgc 1441 ttgcaaaaat tctcaaagtt agcgttgaag aatttagccc ttcaatcgcc agagaaatct 1501 acgagatgta tgaagcggtt agtatgcagc cgtcacttag aagtgagtat gagtaccctg 1561 ttttttctca tgttcaggca gggatgttct cacctgagct tagaaccttt accaaaggtg 1621 atgcggagag atgggtaagc acaaccaaaa aagccagtga ttctgcattc tggcttgagg 1681 ttgaaggtaa ttccatgacc gcaccaacag gctccaagcc aagctttcct gacggaatgt 1741 taattctcgt tgaccctgag caggctgttg agccaggtga tttctgcata gccagacttg 1801 ggggtgatga gtttaccttc aagaaactga tcagggatag cggtcaggtg tttttacaac 1861 cactaaaccc acagtaccca atgatcccat gcaatgagag ttgttccgtt gtggggaaag 1921 ttatcgctag tcagtggcct gaagagacgt ttggcgctgc aaacgacgaa aactacgctt 1981 tagtagctta ataacgctga tagtgctagt gtagatcgct actagagcta gaagatctat 2041 cgtaacgcgt ttactagagg atctgtcgga taacgcgtta ctagagatta aagaggagaa 2101 atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt 2161 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac 2221 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc 2281 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat 2341 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat 2401 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac 2461 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg 2521 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa 2581 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact 2641 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa 2701 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat 2761 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa 2821 ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 2881 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 2941 cgggtgggcc tttctgcgtt tata //