LOCUS BBa_I751112 3334 bp DNA linear 22-Nov-2024 DEFINITION J54140dP + hyprom + lac ACCESSION BBa_I751112 VERSION BBa_I751112.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 158..159 /label=prefix misc_feature 331..331 /label=T misc_feature 649..649 /label=A CDS 158..907 /label=luxR misc_feature 158..913 /label=BBa_C0062 misc_feature 914..938 /label=Help:Barcodes CDS 158..913 /label=BBa_C0062 stem_loop 958..1001 /label=stem_loop misc_feature 947..1026 /label=BBa_B0010 terminator 947..1026 /label=BBa_B0010 stem_loop 1042..1061 /label=T7 TE misc_feature 1068..1068 /label=stop polyA_site 1062..1075 /label=polya misc_feature 1035..1075 /label=BBa_B0012 terminator 1035..1075 /label=BBa_B0012 misc_feature 1084..1088 /label=SalI misc_feature 1098..1102 /label=BamHI misc_feature 1084..1102 /label=BBa_J54033 misc_feature 1158..1176 /label=OR1 promoter 1129..1176 /label=plux-cI hybrid promoter misc_feature 1129..1135 /label=-35 misc_feature 1152..1157 /label=-10 misc_feature 1136..1151 /label=OR2 misc_feature 1111..1128 /label=luxR binding promoter 1111..1176 /label=BBa_I751501 misc_feature 1185..1210 /label=BBa_I751400 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 misc_feature 2326..2364 /label=LVA CDS 1237..2364 /label=lacI-LVA misc_feature 1237..2364 /label=BBa_C0012 misc_feature 2365..2389 /label=Help:Barcodes CDS 1237..2364 /label=BBa_C0012 misc_feature 1219..2389 /label=BBa_S0100 misc_feature 2398..2421 /label=BBa_I751410 misc_feature 2430..2434 /label=BglII misc_feature 2443..2448 /label=MulI misc_feature 2430..2448 /label=BBa_J54025 RBS 2457..2471 /label=RBS-1\Strong RBS 2464..2468 /label=RBS misc_feature 2457..2471 /label=BBa_B0030 RBS 2457..2471 /label=BBa_B0030 CDS 2478..3197 /label=GFP protein CDS 2478..3197 /label=BBa_E0040 stem_loop 3217..3260 /label=stem_loop misc_feature 3206..3285 /label=BBa_B0010 terminator 3206..3285 /label=BBa_B0010 stem_loop 3301..3320 /label=T7 TE misc_feature 3327..3327 /label=stop polyA_site 3321..3334 /label=polya misc_feature 3294..3334 /label=BBa_B0012 terminator 3294..3334 /label=BBa_B0012 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagatg aaaaacataa atgccgacga 181 cacatacaga ataattaata aaattaaagc ttgtagaagc aataatgata ttaatcaatg 241 cttatctgat atgactaaaa tggtacattg tgaatattat ttactcgcga tcatttatcc 301 tcattctatg gttaaatctg atatttcaat cctagataat taccctaaaa aatggaggca 361 atattatgat gacgctaatt taataaaata tgatcctata gtagattatt ctaactccaa 421 tcattcacca attaattgga atatatttga aaacaatgct gtaaataaaa aatctccaaa 481 tgtaattaaa gaagcgaaaa catcaggtct tatcactggg tttagtttcc ctattcatac 541 ggctaacaat ggcttcggaa tgcttagttt tgcacattca gaaaaagaca actatataga 601 tagtttattt ttacatgcgt gtatgaacat accattaatt gttccttctc tagttgataa 661 ttatcgaaaa ataaatatag caaataataa atcaaacaac gatttaacca aaagagaaaa 721 agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg gatatttcaa aaatattagg 781 ttgcagtgag cgtactgtca ctttccattt aaccaatgcg caaatgaaac tcaatacaac 841 aaaccgctgc caaagtattt ctaaagcaat tttaacagga gcaattgatt gcccatactt 901 taaaaattaa taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata 961 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 1021 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 1081 gaggtcgact gacgactgga tctactagag acctgtagga tcgtacaggt ttacgtaaca 1141 ccgtgcgtgt tgatgctttt atcaccgcca gtggtatact agagctagtg tcgactgacg 1201 actggatcca tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg 1261 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 1321 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 1381 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc 1441 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 1501 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 1561 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 1621 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 1681 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 1741 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 1801 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 1861 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 1921 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 1981 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt 2041 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 2101 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 2161 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 2221 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 2281 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa 2341 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagcta 2401 gaagatctat cgtaacgcgt ttactagagg atctgtcgga taacgcgtta ctagagatta 2461 aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc 2521 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag 2581 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg 2641 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc 2701 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg 2761 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg 2821 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa 2881 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag 2941 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa 3001 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt 3061 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga 3121 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg 3181 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 3241 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 3301 ggctcacctt cgggtgggcc tttctgcgtt tata //