BBa_I751112
type: ComponentDefinition
persistent identity: BBa I751112
display id: BBa_I751112
version: 1
was generated by: iGEM to SBOL conversion
description: J54140dP + hyprom + lac
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:08:06Z
owned by: james
owned by: myers
mutable description: A4HPcI + lac
discontinued: false
dominant: false
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Kenichiro Iwasaki
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0010
component: BBa_E0040
component: BBa_B0030
component: BBa_J54025
component: BBa_B0012
component: BBa_J54033
component: BBa_C0062
component: BBa_I751410
component: BBa_S0100
component: BBa_I751400
component: BBa_I751501
component: BBa_R0063
sequence annotation: BBa_R0063
sequence annotation: BBa_C0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_J54033
sequence annotation: BBa_I751501
sequence annotation: BBa_I751400
sequence annotation: BBa_S0100
sequence annotation: BBa_I751410
sequence annotation: BBa_J54025
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_I751112_sequence/1

BBa_B0010
type: Component
persistent identity: component2305056
display id: component2305056
version: 1
top level: BBa_I751112
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component2305055
display id: component2305055
version: 1
top level: BBa_I751112
definition: GFP
access: public

BBa_B0030
type: Component
persistent identity: component2305052
display id: component2305052
version: 1
top level: BBa_I751112
definition: BBa_B0030
access: public

BBa_J54025
type: Component
persistent identity: component2305050
display id: component2305050
version: 1
top level: BBa_I751112
definition: BBa_J54025
access: public

BBa_B0012
type: Component
persistent identity: component2305058
display id: component2305058
version: 1
top level: BBa_I751112
definition: BBa_B0012
access: public

BBa_J54033
type: Component
persistent identity: component2305031
display id: component2305031
version: 1
top level: BBa_I751112
definition: BBa_J54033
access: public

BBa_B0012
type: Component
persistent identity: component2305025
display id: component2305025
version: 1
top level: BBa_I751112
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2305023
display id: component2305023
version: 1
top level: BBa_I751112
definition: BBa_B0010
access: public

BBa_C0062
type: Component
persistent identity: component2305019
display id: component2305019
version: 1
top level: BBa_I751112
definition: luxr
access: public

BBa_I751410
type: Component
persistent identity: component2305047
display id: component2305047
version: 1
top level: BBa_I751112
definition: BBa_I751410
access: public

BBa_S0100
type: Component
persistent identity: component2305046
display id: component2305046
version: 1
top level: BBa_I751112
definition: BBa_S0100
access: public

BBa_I751400
type: Component
persistent identity: component2305039
display id: component2305039
version: 1
top level: BBa_I751112
definition: BBa_I751400
access: public

BBa_I751501
type: Component
persistent identity: component2305038
display id: component2305038
version: 1
top level: BBa_I751112
definition: BBa_I751501
access: public

BBa_R0063
type: Component
persistent identity: component2305012
display id: component2305012
version: 1
top level: BBa_I751112
definition: lux pL
access: public

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation2305012
display id: annotation2305012
version: 1
top level: BBa_I751112
location: https://synbiohub.org/public/igem/BBa_I751112/annotation2305012/range2305012/1
component: BBa_R0063

https://synbiohub.org/public/igem/BBa_I751112/annotation2305012/range2305012/1
type: Range
persistent identity: range2305012
display id: range2305012
version: 1
top level: BBa_I751112
direction: direction/forward
start: 1
end: 151
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2305019
display id: annotation2305019
version: 1
top level: BBa_I751112
location: https://synbiohub.org/public/igem/BBa_I751112/annotation2305019/range2305019/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I751112/annotation2305019/range2305019/1
type: Range
persistent identity: range2305019
display id: range2305019
version: 1
top level: BBa_I751112
direction: direction/forward
start: 158
end: 913
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2305023
display id: annotation2305023
version: 1
top level: BBa_I751112
location: https://synbiohub.org/public/igem/BBa_I751112/annotation2305023/range2305023/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I751112/annotation2305023/range2305023/1
type: Range
persistent identity: range2305023
display id: range2305023
version: 1
top level: BBa_I751112
direction: direction/forward
start: 947
end: 1026
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2305025
display id: annotation2305025
version: 1
top level: BBa_I751112
location: https://synbiohub.org/public/igem/BBa_I751112/annotation2305025/range2305025/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I751112/annotation2305025/range2305025/1
type: Range
persistent identity: range2305025
display id: range2305025
version: 1
top level: BBa_I751112
direction: direction/forward
start: 1035
end: 1075
orientation: inline

BBa_J54033
type: SequenceAnnotation
persistent identity: annotation2305031
display id: annotation2305031
version: 1
top level: BBa_I751112
location: https://synbiohub.org/public/igem/BBa_I751112/annotation2305031/range2305031/1
component: BBa_J54033

https://synbiohub.org/public/igem/BBa_I751112/annotation2305031/range2305031/1
type: Range
persistent identity: range2305031
display id: range2305031
version: 1
top level: BBa_I751112
direction: direction/reverse
start: 1084
end: 1102
orientation: inline

BBa_I751501
type: SequenceAnnotation
persistent identity: annotation2305038
display id: annotation2305038
version: 1
top level: BBa_I751112
location: https://synbiohub.org/public/igem/BBa_I751112/annotation2305038/range2305038/1
component: BBa_I751501

https://synbiohub.org/public/igem/BBa_I751112/annotation2305038/range2305038/1
type: Range
persistent identity: range2305038
display id: range2305038
version: 1
top level: BBa_I751112
direction: direction/reverse
start: 1111
end: 1176
orientation: inline

BBa_I751400
type: SequenceAnnotation
persistent identity: annotation2305039
display id: annotation2305039
version: 1
top level: BBa_I751112
location: https://synbiohub.org/public/igem/BBa_I751112/annotation2305039/range2305039/1
component: BBa_I751400

https://synbiohub.org/public/igem/BBa_I751112/annotation2305039/range2305039/1
type: Range
persistent identity: range2305039
display id: range2305039
version: 1
top level: BBa_I751112
direction: direction/reverse
start: 1185
end: 1210
orientation: inline

BBa_S0100
type: SequenceAnnotation
persistent identity: annotation2305046
display id: annotation2305046
version: 1
top level: BBa_I751112
location: https://synbiohub.org/public/igem/BBa_I751112/annotation2305046/range2305046/1
component: BBa_S0100

https://synbiohub.org/public/igem/BBa_I751112/annotation2305046/range2305046/1
type: Range
persistent identity: range2305046
display id: range2305046
version: 1
top level: BBa_I751112
direction: direction/reverse
start: 1219
end: 2389
orientation: inline

BBa_I751410
type: SequenceAnnotation
persistent identity: annotation2305047
display id: annotation2305047
version: 1
top level: BBa_I751112
location: https://synbiohub.org/public/igem/BBa_I751112/annotation2305047/range2305047/1
component: BBa_I751410

https://synbiohub.org/public/igem/BBa_I751112/annotation2305047/range2305047/1
type: Range
persistent identity: range2305047
display id: range2305047
version: 1
top level: BBa_I751112
direction: direction/reverse
start: 2398
end: 2421
orientation: inline

BBa_J54025
type: SequenceAnnotation
persistent identity: annotation2305050
display id: annotation2305050
version: 1
top level: BBa_I751112
location: https://synbiohub.org/public/igem/BBa_I751112/annotation2305050/range2305050/1
component: BBa_J54025

https://synbiohub.org/public/igem/BBa_I751112/annotation2305050/range2305050/1
type: Range
persistent identity: range2305050
display id: range2305050
version: 1
top level: BBa_I751112
direction: direction/reverse
start: 2430
end: 2448
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2305052
display id: annotation2305052
version: 1
top level: BBa_I751112
location: https://synbiohub.org/public/igem/BBa_I751112/annotation2305052/range2305052/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I751112/annotation2305052/range2305052/1
type: Range
persistent identity: range2305052
display id: range2305052
version: 1
top level: BBa_I751112
direction: direction/forward
start: 2457
end: 2471
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2305055
display id: annotation2305055
version: 1
top level: BBa_I751112
location: https://synbiohub.org/public/igem/BBa_I751112/annotation2305055/range2305055/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I751112/annotation2305055/range2305055/1
type: Range
persistent identity: range2305055
display id: range2305055
version: 1
top level: BBa_I751112
direction: direction/reverse
start: 2478
end: 3197
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2305056
display id: annotation2305056
version: 1
top level: BBa_I751112
location: https://synbiohub.org/public/igem/BBa_I751112/annotation2305056/range2305056/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I751112/annotation2305056/range2305056/1
type: Range
persistent identity: range2305056
display id: range2305056
version: 1
top level: BBa_I751112
direction: direction/forward
start: 3206
end: 3285
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2305058
display id: annotation2305058
version: 1
top level: BBa_I751112
location: https://synbiohub.org/public/igem/BBa_I751112/annotation2305058/range2305058/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I751112/annotation2305058/range2305058/1
type: Range
persistent identity: range2305058
display id: range2305058
version: 1
top level: BBa_I751112
direction: direction/forward
start: 3294
end: 3334
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

BBa_J54033
type: ComponentDefinition
persistent identity: BBa J54033
display id: BBa_J54033
version: 1
was generated by: iGEM to SBOL conversion
description: PrefixII_SalI,BamHI
created: 2006-10-15T11:00:00Z
modified: 2015-08-31T03:48:11Z
mutable provenance: DNA synthesis
owned by: james
owned by: myers
mutable description: Restriction Enzyme site
discontinued: false
dominant: false
group u list: _76_
m user id: 0
owner id: 762
owning group id: 76
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: As prefix
star: false
creator: Yusaku Nakashima
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: SalI
sequence annotation: BamHI
sequence: https://synbiohub.org/public/igem/BBa_J54033_sequence/1

SalI
type: SequenceAnnotation
persistent identity: annotation1903323
display id: annotation1903323
version: 1
top level: BBa_J54033
location: https://synbiohub.org/public/igem/BBa_J54033/annotation1903323/range1903323/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J54033/annotation1903323/range1903323/1
type: Range
persistent identity: range1903323
display id: range1903323
version: 1
top level: BBa_J54033
direction: direction/forward
start: 1
end: 5
orientation: inline

BamHI
type: SequenceAnnotation
persistent identity: annotation1903324
display id: annotation1903324
version: 1
top level: BBa_J54033
location: https://synbiohub.org/public/igem/BBa_J54033/annotation1903324/range1903324/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J54033/annotation1903324/range1903324/1
type: Range
persistent identity: range1903324
display id: range1903324
version: 1
top level: BBa_J54033
direction: direction/forward
start: 15
end: 19
orientation: inline

lux pL
type: ComponentDefinition
persistent identity: BBa R0063
display id: BBa_R0063
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pL)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below.Includes most of Lux reulatory region, including the LuxR binding site which activates the right promoter. A putative LuxR autorepression binding site is also identified adjacent to the -10 site of the right promoter. This 2nd site has 55% identity with the first site. Putative inverted repeats (of size 18-27 bp) also exist between these two sites (not marked above), which may represent binding sites for other regulatory proteins. <img src="<bb_file>Image01.gif</bb_file>" width="614" height="362"> Unspecified.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: LuxR/HSL
sequence annotation: BBa_R0063
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence annotation: Putative LuxR/HSL
sequence: https://synbiohub.org/public/igem/BBa_R0063_sequence/1

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2051
display id: annotation2051
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0063/annotation2051/range2051/1
type: Range
persistent identity: range2051
display id: range2051
version: 1
top level: lux pL
direction: direction/forward
start: 1
end: 20
orientation: inline

BBa_R0063
type: SequenceAnnotation
persistent identity: annotation7071
display id: annotation7071
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0063/annotation7071/range7071/1
type: Range
persistent identity: range7071
display id: range7071
version: 1
top level: lux pL
direction: direction/forward
start: 1
end: 151
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2053
display id: annotation2053
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0063/annotation2053/range2053/1
type: Range
persistent identity: range2053
display id: range2053
version: 1
top level: lux pL
direction: direction/forward
start: 89
end: 94
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2052
display id: annotation2052
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0063/annotation2052/range2052/1
type: Range
persistent identity: range2052
display id: range2052
version: 1
top level: lux pL
direction: direction/forward
start: 115
end: 122
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2054
display id: annotation2054
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_R0063/annotation2054/range2054/1
type: Range
persistent identity: range2054
display id: range2054
version: 1
top level: lux pL
direction: direction/forward
start: 128
end: 128
orientation: inline

Putative LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2055
display id: annotation2055
version: 1
top level: lux pL
location: https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0063/annotation2055/range2055/1
type: Range
persistent identity: range2055
display id: range2055
version: 1
top level: lux pL
direction: direction/forward
start: 130
end: 149
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_S0100
type: ComponentDefinition
persistent identity: BBa S0100
display id: BBa_S0100
version: 1
was generated by: iGEM to SBOL conversion
description: B0034.C0012
created: 2003-12-04T12:00:00Z
modified: 2015-05-08T01:14:16Z
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: true
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_C0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0012
sequence: https://synbiohub.org/public/igem/BBa_S0100_sequence/1

BBa_B0034
type: Component
persistent identity: component939698
display id: component939698
version: 1
top level: BBa_S0100
definition: BBa_B0034
access: public

BBa_C0012
type: Component
persistent identity: component939708
display id: component939708
version: 1
top level: BBa_S0100
definition: lacI
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation939698
display id: annotation939698
version: 1
top level: BBa_S0100
location: https://synbiohub.org/public/igem/BBa_S0100/annotation939698/range939698/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S0100/annotation939698/range939698/1
type: Range
persistent identity: range939698
display id: range939698
version: 1
top level: BBa_S0100
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation939708
display id: annotation939708
version: 1
top level: BBa_S0100
location: https://synbiohub.org/public/igem/BBa_S0100/annotation939708/range939708/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_S0100/annotation939708/range939708/1
type: Range
persistent identity: range939708
display id: range939708
version: 1
top level: BBa_S0100
direction: direction/forward
start: 19
end: 1146
orientation: inline

BBa_I751400
type: ComponentDefinition
persistent identity: BBa I751400
display id: BBa_I751400
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick to Tokyo Standard adapter, for XbaI, forward
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:06Z
owned by: james
owned by: myers
mutable description: This part is oligonucleotide you can buy from oligo house. Annealed with rev oligo, this part can be incorporated into the space cut by XbaI. This makes Biobrick part into Tokyo Standard part.
discontinued: false
dominant: false
experience: experience/None
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: *BBtoTSD adapter 1. Cut Biobrick part with XbaI. 2. Incorporate adapter oligo. (don't forget to anneal with rev, and to make kination) 3. Now you can cut out this part by SalI or BamHI. This makes Biobrick scar-like sequence, "tctagt" in the left and "actaga" in the right. Once oligo is ligated, you can't cut with XbaI anymore.
star: false
creator: Kenichiro Iwasaki
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_I751400_sequence/1

BBa_I751410
type: ComponentDefinition
persistent identity: BBa I751410
display id: BBa_I751410
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick to Tokyo Standard adapter, for SpeI, forward
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:06Z
owned by: james
owned by: myers
mutable description: This part is oligonucleotide you can buy from oligo house. Annealed with rev oligo(BBa_I751411), this part can be incorporated into the space cut by SpeI. This makes Biobrick part into Tokyo Standard part.
discontinued: false
dominant: false
experience: experience/None
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: *BBtoTSD adapter 1. Cut Biobrick part with SpeI. 2. Incorporate adapter oligo. (don't forget to anneal with rev, and to make kination) 3. Now you can cut out this part by BglII or MluI. This makes Biobrick scar-like sequence, "actaga" in the left and "tctagt" in the right. Once oligo is ligated, you can't cut with SpeI anymore.
star: false
creator: Kenichiro Iwasaki
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_I751410_sequence/1

BBa_J54025
type: ComponentDefinition
persistent identity: BBa J54025
display id: BBa_J54025
version: 1
was generated by: iGEM to SBOL conversion
description: SuffixI_BglII,MluI
created: 2006-10-15T11:00:00Z
modified: 2015-08-31T03:48:11Z
mutable provenance: DNA synthesis
owned by: james
owned by: myers
mutable description: Restriction Enzyme site
discontinued: false
dominant: false
group u list: _76_
m user id: 0
owner id: 762
owning group id: 76
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: As Suffix
star: false
creator: Yusaku Nakashima
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence annotation: BglII
sequence annotation: MulI
sequence: https://synbiohub.org/public/igem/BBa_J54025_sequence/1

BglII
type: SequenceAnnotation
persistent identity: annotation1903321
display id: annotation1903321
version: 1
top level: BBa_J54025
location: https://synbiohub.org/public/igem/BBa_J54025/annotation1903321/range1903321/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J54025/annotation1903321/range1903321/1
type: Range
persistent identity: range1903321
display id: range1903321
version: 1
top level: BBa_J54025
direction: direction/forward
start: 1
end: 5
orientation: inline

MulI
type: SequenceAnnotation
persistent identity: annotation1903322
display id: annotation1903322
version: 1
top level: BBa_J54025
location: https://synbiohub.org/public/igem/BBa_J54025/annotation1903322/range1903322/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J54025/annotation1903322/range1903322/1
type: Range
persistent identity: range1903322
display id: range1903322
version: 1
top level: BBa_J54025
direction: direction/forward
start: 14
end: 19
orientation: inline

BBa_I751501
type: ComponentDefinition
persistent identity: BBa I751501
display id: BBa_I751501
version: 1
was generated by: iGEM to SBOL conversion
description: plux-cI hybrid promoter
created: 2007-10-24T11:00:00Z
modified: 2015-08-31T04:08:06Z
owned by: james
owned by: myers
mutable description: sense TWO INPUTS, activation by AHL and repression by LacI.
discontinued: false
dominant: false
experience: experience/Works
group u list: _151_
m user id: 0
owner id: 2188
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Based on the AHL regulated BioBrick part R0062 and the previous work on LacI regulated promoter (ref. 1), we designed a new promoter regulated by AHL and LacI. Since this promoter is a kind of a "HYBRID" of the two, we call it HYBRID PROMOTER. Compared with existing BioBrick hybrid promoter R0065, this promoter is different in that its sequence is much shorter with lux box and -35 region overlapping, and that it is repressed by LacI, not cI. Also, the output is clearly detected as shown in Fig. 3.
star: false
creator: Kenichiro Iwasaki
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: luxR binding
sequence annotation: plux-cI hybrid promoter
sequence annotation: -35
sequence annotation: OR2
sequence annotation: -10
sequence annotation: OR1
sequence: https://synbiohub.org/public/igem/BBa_I751501_sequence/1

luxR binding
type: SequenceAnnotation
persistent identity: annotation1955482
display id: annotation1955482
version: 1
top level: BBa_I751501
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955482/range1955482/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I751501/annotation1955482/range1955482/1
type: Range
persistent identity: range1955482
display id: range1955482
version: 1
top level: BBa_I751501
direction: direction/forward
start: 1
end: 18
orientation: inline

plux-cI hybrid promoter
type: SequenceAnnotation
persistent identity: annotation1955478
display id: annotation1955478
version: 1
top level: BBa_I751501
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955478/range1955478/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I751501/annotation1955478/range1955478/1
type: Range
persistent identity: range1955478
display id: range1955478
version: 1
top level: BBa_I751501
direction: direction/forward
start: 19
end: 66
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1955479
display id: annotation1955479
version: 1
top level: BBa_I751501
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955479/range1955479/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I751501/annotation1955479/range1955479/1
type: Range
persistent identity: range1955479
display id: range1955479
version: 1
top level: BBa_I751501
direction: direction/forward
start: 19
end: 25
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation1955481
display id: annotation1955481
version: 1
top level: BBa_I751501
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955481/range1955481/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I751501/annotation1955481/range1955481/1
type: Range
persistent identity: range1955481
display id: range1955481
version: 1
top level: BBa_I751501
direction: direction/forward
start: 26
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1955480
display id: annotation1955480
version: 1
top level: BBa_I751501
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955480/range1955480/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I751501/annotation1955480/range1955480/1
type: Range
persistent identity: range1955480
display id: range1955480
version: 1
top level: BBa_I751501
direction: direction/forward
start: 42
end: 47
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation1955477
display id: annotation1955477
version: 1
top level: BBa_I751501
location: https://synbiohub.org/public/igem/BBa_I751501/annotation1955477/range1955477/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I751501/annotation1955477/range1955477/1
type: Range
persistent identity: range1955477
display id: range1955477
version: 1
top level: BBa_I751501
direction: direction/forward
start: 48
end: 66
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

https://synbiohub.org/public/igem/BBa_I751112_sequence/1
type: Sequence
persistent identity: BBa I751112 sequence
display id: BBa_I751112_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggtcgactgacgactggatctactagagacctgtaggatcgtacaggtttacgtaacaccgtgcgtgttgatgcttttatcaccgccagtggtatactagagctagtgtcgactgacgactggatccatactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctggg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J54033_sequence/1
type: Sequence
persistent identity: BBa J54033 sequence
display id: BBa_J54033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtcgactgacgactggatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S0100_sequence/1
type: Sequence
persistent identity: BBa S0100 sequence
display id: BBa_S0100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I751400_sequence/1
type: Sequence
persistent identity: BBa I751400 sequence
display id: BBa_I751400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagtgtcgactgacgactggatcca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I751410_sequence/1
type: Sequence
persistent identity: BBa I751410 sequence
display id: BBa_I751410_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagaagatctatcgtaacgcgtt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0063_sequence/1
type: Sequence
persistent identity: BBa R0063 sequence
display id: BBa_R0063_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J54025_sequence/1
type: Sequence
persistent identity: BBa J54025 sequence
display id: BBa_J54025_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatctgtcggataacgcgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I751501_sequence/1
type: Sequence
persistent identity: BBa I751501 sequence
display id: BBa_I751501_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgtaacaccgtgcgtgttgatgcttttatcaccgccagtggta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z