LOCUS       BBa_I751311              963 bp    DNA     linear       16-Feb-2025
DEFINITION  pBR322 pcI-GFP
ACCESSION   BBa_I751311
VERSION     BBa_I751311.1  
FEATURES             Location/Qualifiers
     promoter        1..77
                     /label=pcI
     misc_feature    21..26
                     /label=-35
     misc_feature    47..50
                     /label=-10
     misc_feature    1..20
                     /label=OR3
     misc_feature    27..46
                     /label=OR2
     misc_feature    51..77
                     /label=OR1
     promoter        1..77
                     /label=BBa_I751500
     RBS             86..100
                     /label=RBS-1\Strong
     RBS             93..97
                     /label=RBS
     misc_feature    86..100
                     /label=BBa_B0030
     RBS             86..100
                     /label=BBa_B0030
     CDS             107..826
                     /label=GFP protein
     CDS             107..826
                     /label=BBa_E0040
     stem_loop       846..889
                     /label=stem_loop
     misc_feature    835..914
                     /label=BBa_B0010
     terminator      835..914
                     /label=BBa_B0010
     stem_loop       930..949
                     /label=T7 TE
     misc_feature    956..956
                     /label=stop
     polyA_site      950..963
                     /label=polya
     misc_feature    923..963
                     /label=BBa_B0012
     terminator      923..963
                     /label=BBa_B0012
ORIGIN
        1 atctatcacc gcaagggata aatatctaac acaccgtgcg ttgactattt tacctctggc
       61 ggtgataatg gttgcagtac tagagattaa agaggagaaa tactagatgc gtaaaggaga
      121 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      181 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      241 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      301 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      361 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      421 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      481 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      541 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      601 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      661 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
      721 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
      781 agctgctggg attacacatg gcatggatga actatacaaa taataatact agagccaggc
      841 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      901 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      961 ata
//