BBa_I751410
1
BBa_I751410
BioBrick to Tokyo Standard adapter, for SpeI, forward
2007-10-24T11:00:00Z
2015-08-31T04:08:06Z
This part is oligonucleotide you can buy from oligo house.
Annealed with rev oligo(BBa_I751411), this part can be incorporated into the space cut by SpeI.
This makes Biobrick part into Tokyo Standard part.
false
false
_151_
0
2188
9
Not in stock
false
*[[BBtoTSD adapter]]
1. Cut Biobrick part with SpeI.
2. Incorporate adapter oligo. (don't forget to anneal with rev, and to make kination)
3. Now you can cut out this part by BglII or MluI.
This makes Biobrick scar-like sequence, "actaga" in the left and "tctagt" in the right. Once oligo is ligated, you can't cut with SpeI anymore.
false
Kenichiro Iwasaki
BBa_I751410_sequence
1
ctagaagatctatcgtaacgcgtt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z