BBa_I751501
1
BBa_I751501
plux-cI hybrid promoter
2007-10-24T11:00:00Z
2015-08-31T04:08:06Z
sense TWO INPUTS, activation by AHL and repression by LacI.
false
false
_151_
0
2188
9
Not in stock
false
Based on the AHL regulated BioBrick part R0062 and the previous work on LacI regulated promoter (ref. 1), we designed a new promoter regulated by AHL and LacI. Since this promoter is a kind of a "HYBRID" of the two, we call it HYBRID PROMOTER.
Compared with existing BioBrick hybrid promoter R0065, this promoter is different in that its sequence is much shorter with lux box and -35 region overlapping, and that it is repressed by LacI, not cI.
Also, the output is clearly detected as shown in Fig. 3.
false
Kenichiro Iwasaki
annotation1955478
1
plux-cI hybrid promoter
range1955478
1
19
66
annotation1955479
1
-35
range1955479
1
19
25
annotation1955482
1
luxR binding
range1955482
1
1
18
annotation1955477
1
OR1
range1955477
1
48
66
annotation1955480
1
-10
range1955480
1
42
47
annotation1955481
1
OR2
range1955481
1
26
41
BBa_I751501_sequence
1
acctgtaggatcgtacaggtttacgtaacaccgtgcgtgttgatgcttttatcaccgccagtggta
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z