BBa_I751502
1
BBa_I751502
plux-lac hybrid promoter
2007-10-24T11:00:00Z
2015-08-31T04:08:06Z
sense TWO INPUTS, activation by AHL and repression by LacI.
false
false
_151_
0
2188
9
Not in stock
false
Compared with existing BioBrick hybrid promoter R0065, this promoter is different in that its sequence is much shorter with lux box and -35 region overlapping, and that it is repressed by LacI, not cI.
Also, the output is clearly detected as shown in Fig. 3.
false
Kenichiro Iwasaki
annotation1955563
1
O1
range1955563
1
49
74
annotation1955564
1
luxR binding site
range1955564
1
1
19
annotation1955559
1
plac
range1955559
1
20
74
annotation1955562
1
O1
range1955562
1
27
41
annotation1955561
1
-10
range1955561
1
42
48
annotation1955560
1
-35
range1955560
1
20
26
BBa_I751900
1
BBa_I751900
plux-lac + GFP
2007-10-25T11:00:00Z
2015-08-31T04:08:06Z
This part is made for AHL assay, producing GFP as a reporter.
false
false
_151_
0
2188
9
Not in stock
false
false
Kenichiro Iwasaki
component1957074
1
BBa_J54100
component1957069
1
BBa_I751502
component1957076
1
BBa_E0040
annotation1957069
1
BBa_I751502
range1957069
1
1
74
annotation1957074
1
BBa_J54100
range1957074
1
83
169
annotation1957076
1
BBa_E0040
range1957076
1
176
895
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_J54100
1
BBa_J54100
Prefix-Suffix(deltaP)
2006-10-14T11:00:00Z
2015-08-31T03:48:11Z
DNA synthesis
Restriction Enzyme site
false
false
_76_
0
762
76
Not in stock
false
Promoter-less
false
Yusaku Nakashima
annotation1903204
1
MluI
range1903204
1
57
62
annotation1903202
1
BamHI
range1903202
1
32
37
annotation1903201
1
SalI
range1903201
1
18
23
annotation1903203
1
BglII
range1903203
1
44
49
BBa_J54100_sequence
1
cgcggccgcttctagaggtcgactgacgactggatcctgtcggagatctgtcggataacgcgtgagattaaagaggagaaatactag
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_I751900_sequence
1
acctgtaggatcgtacaggtttacttgtgagcggataacaatatagtgtgtggaattgtgagcggataacaatttactagagcgcggccgcttctagaggtcgactgacgactggatcctgtcggagatctgtcggataacgcgtgagattaaagaggagaaatactagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_I751502_sequence
1
acctgtaggatcgtacaggtttacttgtgagcggataacaatatagtgtgtggaattgtgagcggataacaatt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z