BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_I752001
1
BBa_I752001
CheZ coding sequence (Chemotaxis protein)
2007-08-26T11:00:00Z
2016-10-08T03:28:13Z
blah
CheZ is one of the 6 Che proteins used in chemeotaxis. CheZ is a phosphatase which dephosphorylates CheY which in turn then allows the cell to run and tumble, wheres if CheZ is absence, CheY is phosphorylated, and the cell tumbles exclusively. (Topp and Gallivan J. Am. Chem. Soc. 129, p6865-6873)
false
false
_129_
25212
1631
9
It's complicated
false
blah
false
David Franz
annotation1944568
1
CheZ
range1944568
1
1
639
BBa_I752000
1
BBa_I752000
Riboswitch(theophylline)
2007-08-26T11:00:00Z
2015-08-31T04:08:06Z
The part was publish by Topp and Gallivan (J. Am. Chem. Soc. 129, p 6865-6873)
The riboswitch changes conformation depending on the presence of theophylline. In the absence of theophylline, the switch prevents the binding of the mRNA to the ribosome whilst in the presence of said chemical, the conformation changes and the mRNA behind the switch can then be expressed.
false
true
_129_
0
1631
9
It's complicated
false
After sequence information was acquired for the switch from Topp and Gallivan (J. Am. Chem. Soc. 129, p 6865-6873), part was created with required prefix and suffix sequences by gene synthesis (by company XX)
true
David Franz
annotation1944567
1
Riboswitch
range1944567
1
1
56
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_I752003
1
BBa_I752003
full RS+CheZ
2007-09-24T11:00:00Z
2015-08-31T04:08:06Z
The switch was published by Topp and Gallivan
CheZ encoding regulation by theophyline riboswithc with stop codons
false
false
_129_
0
1631
9
Not in stock
false
blank
false
David Franz
component1944652
1
BBa_B0012
component1944650
1
BBa_B0010
component1944647
1
BBa_I752000
component1944649
1
BBa_I752001
component1944645
1
BBa_J23119
annotation1944649
1
BBa_I752001
range1944649
1
106
744
annotation1944647
1
BBa_I752000
range1944647
1
44
99
annotation1944650
1
BBa_B0010
range1944650
1
753
832
annotation1944652
1
BBa_B0012
range1944652
1
841
881
annotation1944645
1
BBa_J23119
range1944645
1
1
35
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_I752000_sequence
1
ggtgataccagcatcgtcttgatgcccttggcagcaccccgctgcaagacaacaag
BBa_I752001_sequence
1
atgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctgccagatgtacccgcgcataccagctttactaacgggcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtcttggattt
BBa_I752003_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagggtgataccagcatcgtcttgatgcccttggcagcaccccgctgcaagacaacaagtactagatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctgccagatgtacccgcgcataccagctttactaacgggcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtcttggattttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z