LOCUS BBa_I754002 4421 bp DNA linear 02-May-2025 DEFINITION Trial ACCESSION BBa_I754002 VERSION BBa_I754002.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..77 /label=RBS-1\Strong RBS 70..74 /label=RBS misc_feature 63..77 /label=BBa_B0030 RBS 63..77 /label=BBa_B0030 misc_feature 84..149 /label=PelB misc_feature 84..149 /label=BBa_J32015 CDS 156..875 /label=GFP protein CDS 156..875 /label=BBa_E0040 CDS 882..1601 /label=GFP protein CDS 882..1601 /label=BBa_E0040 CDS 1608..2327 /label=GFP protein CDS 1608..2327 /label=BBa_E0040 CDS 2334..3053 /label=GFP protein CDS 2334..3053 /label=BBa_E0040 misc_feature 3062..3107 /label=S-Tag misc_feature 3062..3107 /label=BBa_J32017 CDS 3161..3952 /label=SpR Spectinomycin Resistance misc_feature 3116..4003 /label=BBa_J23012 misc_feature 4358..4390 /label=AAV CDS 4010..4357 /label=Xis p22 misc_feature 4391..4396 /label=stop misc_feature 4397..4421 /label=Help:Barcodes CDS 4010..4396 /label=BBa_I11031 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agattaaaga ggagaaatac tagatgaaat acctgctgcc gaccgctgct gctggtctgc 121 tgctcctcgc tgcccagccg gcgatggcct actagatgcg taaaggagaa gaacttttca 181 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg 241 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca 301 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc 361 aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc 421 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac 481 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg 541 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca 601 atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac 661 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg 721 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga 781 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga 841 ttacacatgg catggatgaa ctatacaaat aataatacta gatgcgtaaa ggagaagaac 901 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat 961 tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta 1021 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg 1081 gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg 1141 ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca 1201 agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag 1261 gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac aactataact 1321 cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa 1381 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc 1441 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc 1501 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg 1561 ctgggattac acatggcatg gatgaactat acaaataata atactagatg cgtaaaggag 1621 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc 1681 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta 1741 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg 1801 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca 1861 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga 1921 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt 1981 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact 2041 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact 2101 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa 2161 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat 2221 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa 2281 cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac tagatgcgta 2341 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta 2401 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta 2461 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta 2521 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact 2581 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg 2641 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa 2701 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat 2761 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag 2821 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc 2881 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca 2941 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt 3001 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa taatactaga 3061 gaaaagaaac cgctgctgct aaattcgaac gccagcacat ggacagctac tagagtccaa 3121 gcgagctcga tatccgtcgg cttgaacgaa ttgttagaca ttatttgccg actaccttgg 3181 tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc 3241 gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg 3301 ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta 3361 ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt 3421 cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag 3481 gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg 3541 cttttgtcag caagatagcc agatcaatgt cgtacgtggc tggctcgaag atacctgcaa 3601 gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg 3661 gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc 3721 caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa 3781 gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca 3841 ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc 3901 caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc atgatgttta 3961 actttgtttt agggcgactg ccctgctgcg taacatcgtt gcttactaga tggaatcaca 4021 cagcctcaca cttgatgagg cctgtgcatt tctcaagata tccagaccta ccgccaccaa 4081 ctggattcgc acaggccgac tacaggcaac acgtaaagac cccaccaaac cgaaatcccc 4141 ttacctcacc acacgacaag cctgcattgc ggcacttcaa tctccgctgc atactgtcca 4201 ggtgagcgcg ggtgatgaca taacagagga actgaaatgt cactattccg cagaggtgaa 4261 acctggtacg ccagtttcac attgccgaac ggcaaaagat ttaagcagtc tcttgggaca 4321 aaggacaaaa ggcaggccac agagcttcat gacaagcgca gcaaacgacg aaaactacgc 4381 tgctgctgtt taataacgct gatagtgcta gtgtagatcg c //