##gff-version 3
##sequence-region BBa_I754002 1 4396
BBa_I754002	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I754002	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I754002	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I754002	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I754002	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I754002	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I754002	.	CDS	156	875	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_I754002	.	CDS	156	875	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I754002	.	engineered_region	3116	4003	.	+	0	ID=BBa_J23012;Name=BBa_J23012
BBa_I754002	.	CDS	3161	3952	.	+	0	ID=annotation1966465;Name=SpR Spectinomycin Resistance;Parent=BBa_J23012
BBa_I754002	.	CDS	4010	4396	.	+	0	ID=BBa_I11031;Name=BBa_I11031
BBa_I754002	.	CDS	4010	4357	.	+	0	ID=annotation1198186;Name=Xis p22;Parent=BBa_I11031
BBa_I754002	.	tag	4358	4390	.	+	0	ID=annotation1064115;Name=AAV;Parent=BBa_I11031
BBa_I754002	.	engineered_tag	4397	4421	.	+	0	ID=annotation2214030;Name=Help:Barcodes;Parent=BBa_I11031
BBa_I754002	.	stop_codon	4391	4396	.	+	0	ID=annotation1198221;Name=stop;Parent=BBa_I11031
BBa_I754002	.	CDS	2334	3053	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_I754002	.	CDS	2334	3053	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I754002	.	tag	84	149	.	+	0	ID=BBa_J32015;Name=BBa_J32015
BBa_I754002	.	tag	84	149	.	+	0	ID=annotation1898182;Name=PelB;Parent=BBa_J32015
BBa_I754002	.	ribosome_entry_site	63	77	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I754002	.	engineered_region	63	77	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I754002	.	ribosome_entry_site	70	74	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I754002	.	ribosome_entry_site	63	77	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I754002	.	CDS	882	1601	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_I754002	.	CDS	882	1601	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I754002	.	CDS	1608	2327	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_I754002	.	CDS	1608	2327	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_I754002	.	tag	3062	3107	.	+	0	ID=BBa_J32017;Name=BBa_J32017
BBa_I754002	.	tag	3062	3107	.	+	0	ID=annotation1898184;Name=S-Tag;Parent=BBa_J32017
>BBa_I754002
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaata
ctagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcctactagatg
cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaatttt
ctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacc
tgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacag
catgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactaca
agacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaaga
tggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaat
ggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaata
ctccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaa
cgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaa
taataatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgtta
atgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcac
tactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagataccca
gatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaag
atgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtat
tgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggca
gacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagacc
attatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccct
ttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatg
gatgaactatacaaataataatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaat
tagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccct
taaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgc
tttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaa
ctatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaat
cgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaat
gtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcg
ttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtc
cacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctggg
attacacatggcatggatgaactatacaaataataatactagatgcgtaaaggagaagaacttttcactggagttgtcc
caattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacata
cggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggt
tatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggtt
atgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatac
ccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaac
tataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaaca
ttgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccaga
caaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagttt
gtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagaaaagaaaccgctgctgcta
aattcgaacgccagcacatggacagctactagagtccaagcgagctcgatatccgtcggcttgaacgaattgttagaca
ttatttgccgactaccttggtgatctcgcctttcacgtagtggacaaattcttccaactgatctgcgcgcgaggccaag
cgatcttcttcttgtccaagataagcctgtctagcttcaagtatgacgggctgatactgggccggcaggcgctccattg
cccagtcggcagcgacatccttcggcgcgattttgccggttactgcgctgtaccaaatgcgggacaacgtaagcactac
atttcgctcatcgccagcccagtcgggcggcgagttccatagcgttaaggtttcatttagcgcctcaaatagatcctgt
tcaggaaccggatcaaagagttcctccgccgctggacctaccaaggcaacgctatgttctcttgcttttgtcagcaaga
tagccagatcaatgtcgtacgtggctggctcgaagatacctgcaagaatgtcattgcgctgccattctccaaattgcag
ttcgcgcttagctggataacgccacggaatgatgtcgtcgtgcacaacaatggtgacttctacagcgcggagaatctcg
ctctctccaggggaagccgaagtttccaaaaggtcgttgatcaaagctcgccgcgttgtttcatcaagccttacggtca
ccgtaaccagcaaatcaatatcactgtgtggcttcaggccgccatccactgcggagccgtacaaatgtacggccagcaa
cgtcggttcgagatggcgctcgatgacgccaactacctctgatagttgagtcgatacttcggcgatcaccgcttccctc
atgatgtttaactttgttttagggcgactgccctgctgcgtaacatcgttgcttactagatggaatcacacagcctcac
acttgatgaggcctgtgcatttctcaagatatccagacctaccgccaccaactggattcgcacaggccgactacaggca
acacgtaaagaccccaccaaaccgaaatccccttacctcaccacacgacaagcctgcattgcggcacttcaatctccgc
tgcatactgtccaggtgagcgcgggtgatgacataacagaggaactgaaatgtcactattccgcagaggtgaaacctgg
tacgccagtttcacattgccgaacggcaaaagatttaagcagtctcttgggacaaaggacaaaaggcaggccacagagc
ttcatgacaagcgcagcaaacgacgaaaactacgctgctgctgtttaataacgctgatagtgctagtgtagatcgc