BBa_I754002
type: ComponentDefinition
persistent identity: BBa I754002
display id: BBa_I754002
version: 1
was generated by: iGEM to SBOL conversion
description: Trial
created: 2007-08-25T11:00:00Z
modified: 2015-08-31T04:08:06Z
mutable provenance: trial
owned by: james
owned by: myers
mutable description: trial
discontinued: true
dominant: false
experience: experience/None
group u list: _154_
m user id: 0
owner id: 1490
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: trial
star: false
creator: KORAY KIRLI
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_R0040
component: BBa_J32015
component: BBa_B0030
component: BBa_E0040
component: BBa_J23012
component: BBa_J32017
component: BBa_I11031
sequence annotation: BBa_R0040
sequence annotation: BBa_B0030
sequence annotation: BBa_J32015
sequence annotation: BBa_E0040
sequence annotation: BBa_J32017
sequence annotation: BBa_J23012
sequence annotation: BBa_I11031
sequence: https://synbiohub.org/public/igem/BBa_I754002_sequence/1

BBa_R0040
type: Component
persistent identity: component1942493
display id: component1942493
version: 1
top level: BBa_I754002
definition: p(tetR)
access: public

BBa_J32015
type: Component
persistent identity: component1942501
display id: component1942501
version: 1
top level: BBa_I754002
definition: PelB
access: public

BBa_B0030
type: Component
persistent identity: component1942498
display id: component1942498
version: 1
top level: BBa_I754002
definition: BBa_B0030
access: public

BBa_E0040
type: Component
persistent identity: component1942505
display id: component1942505
version: 1
top level: BBa_I754002
definition: GFP
access: public

BBa_E0040
type: Component
persistent identity: component1942503
display id: component1942503
version: 1
top level: BBa_I754002
definition: GFP
access: public

BBa_E0040
type: Component
persistent identity: component1942509
display id: component1942509
version: 1
top level: BBa_I754002
definition: GFP
access: public

BBa_E0040
type: Component
persistent identity: component1942507
display id: component1942507
version: 1
top level: BBa_I754002
definition: GFP
access: public

BBa_J23012
type: Component
persistent identity: component1942513
display id: component1942513
version: 1
top level: BBa_I754002
definition: BBa_J23012
access: public

BBa_J32017
type: Component
persistent identity: component1942511
display id: component1942511
version: 1
top level: BBa_I754002
definition: S*Tag
access: public

BBa_I11031
type: Component
persistent identity: component1942517
display id: component1942517
version: 1
top level: BBa_I754002
definition: BBa_I11031
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1942493
display id: annotation1942493
version: 1
top level: BBa_I754002
location: https://synbiohub.org/public/igem/BBa_I754002/annotation1942493/range1942493/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I754002/annotation1942493/range1942493/1
type: Range
persistent identity: range1942493
display id: range1942493
version: 1
top level: BBa_I754002
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1942498
display id: annotation1942498
version: 1
top level: BBa_I754002
location: https://synbiohub.org/public/igem/BBa_I754002/annotation1942498/range1942498/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I754002/annotation1942498/range1942498/1
type: Range
persistent identity: range1942498
display id: range1942498
version: 1
top level: BBa_I754002
direction: direction/forward
start: 63
end: 77
orientation: inline

BBa_J32015
type: SequenceAnnotation
persistent identity: annotation1942501
display id: annotation1942501
version: 1
top level: BBa_I754002
location: https://synbiohub.org/public/igem/BBa_I754002/annotation1942501/range1942501/1
component: BBa_J32015

https://synbiohub.org/public/igem/BBa_I754002/annotation1942501/range1942501/1
type: Range
persistent identity: range1942501
display id: range1942501
version: 1
top level: BBa_I754002
direction: direction/reverse
start: 84
end: 149
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1942503
display id: annotation1942503
version: 1
top level: BBa_I754002
location: https://synbiohub.org/public/igem/BBa_I754002/annotation1942503/range1942503/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I754002/annotation1942503/range1942503/1
type: Range
persistent identity: range1942503
display id: range1942503
version: 1
top level: BBa_I754002
direction: direction/reverse
start: 156
end: 875
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1942505
display id: annotation1942505
version: 1
top level: BBa_I754002
location: https://synbiohub.org/public/igem/BBa_I754002/annotation1942505/range1942505/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I754002/annotation1942505/range1942505/1
type: Range
persistent identity: range1942505
display id: range1942505
version: 1
top level: BBa_I754002
direction: direction/reverse
start: 882
end: 1601
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1942507
display id: annotation1942507
version: 1
top level: BBa_I754002
location: https://synbiohub.org/public/igem/BBa_I754002/annotation1942507/range1942507/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I754002/annotation1942507/range1942507/1
type: Range
persistent identity: range1942507
display id: range1942507
version: 1
top level: BBa_I754002
direction: direction/reverse
start: 1608
end: 2327
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1942509
display id: annotation1942509
version: 1
top level: BBa_I754002
location: https://synbiohub.org/public/igem/BBa_I754002/annotation1942509/range1942509/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I754002/annotation1942509/range1942509/1
type: Range
persistent identity: range1942509
display id: range1942509
version: 1
top level: BBa_I754002
direction: direction/reverse
start: 2334
end: 3053
orientation: inline

BBa_J32017
type: SequenceAnnotation
persistent identity: annotation1942511
display id: annotation1942511
version: 1
top level: BBa_I754002
location: https://synbiohub.org/public/igem/BBa_I754002/annotation1942511/range1942511/1
component: BBa_J32017

https://synbiohub.org/public/igem/BBa_I754002/annotation1942511/range1942511/1
type: Range
persistent identity: range1942511
display id: range1942511
version: 1
top level: BBa_I754002
direction: direction/reverse
start: 3062
end: 3107
orientation: inline

BBa_J23012
type: SequenceAnnotation
persistent identity: annotation1942513
display id: annotation1942513
version: 1
top level: BBa_I754002
location: https://synbiohub.org/public/igem/BBa_I754002/annotation1942513/range1942513/1
component: BBa_J23012

https://synbiohub.org/public/igem/BBa_I754002/annotation1942513/range1942513/1
type: Range
persistent identity: range1942513
display id: range1942513
version: 1
top level: BBa_I754002
direction: direction/reverse
start: 3116
end: 4003
orientation: inline

BBa_I11031
type: SequenceAnnotation
persistent identity: annotation1942517
display id: annotation1942517
version: 1
top level: BBa_I754002
location: https://synbiohub.org/public/igem/BBa_I754002/annotation1942517/range1942517/1
component: BBa_I11031

https://synbiohub.org/public/igem/BBa_I754002/annotation1942517/range1942517/1
type: Range
persistent identity: range1942517
display id: range1942517
version: 1
top level: BBa_I754002
direction: direction/reverse
start: 4010
end: 4396
orientation: inline

BBa_I11031
type: ComponentDefinition
persistent identity: BBa I11031
display id: BBa_I11031
version: 1
was generated by: iGEM to SBOL conversion
description: excisionase from E. coli phage P22 (removes prophage from host genome)
created: 2004-09-02T11:00:00Z
modified: 2015-08-31T04:07:30Z
mutable provenance: Nuc Acid Res, 2001; 29(11):2205-2216
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: P22 Excisionase with AAV tag
discontinued: false
dominant: false
experience: experience/None
group u list: _2_
m user id: 0
owner id: 102
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: mschomp
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Xis p22
sequence annotation: AAV
sequence annotation: stop
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I11031_sequence/1

Xis p22
type: SequenceAnnotation
persistent identity: annotation1198186
display id: annotation1198186
version: 1
top level: BBa_I11031
location: https://synbiohub.org/public/igem/BBa_I11031/annotation1198186/range1198186/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I11031/annotation1198186/range1198186/1
type: Range
persistent identity: range1198186
display id: range1198186
version: 1
top level: BBa_I11031
direction: direction/forward
start: 1
end: 348
orientation: inline

AAV
type: SequenceAnnotation
persistent identity: annotation1064115
display id: annotation1064115
version: 1
top level: BBa_I11031
location: https://synbiohub.org/public/igem/BBa_I11031/annotation1064115/range1064115/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_I11031/annotation1064115/range1064115/1
type: Range
persistent identity: range1064115
display id: range1064115
version: 1
top level: BBa_I11031
direction: direction/forward
start: 349
end: 381
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1198221
display id: annotation1198221
version: 1
top level: BBa_I11031
location: https://synbiohub.org/public/igem/BBa_I11031/annotation1198221/range1198221/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I11031/annotation1198221/range1198221/1
type: Range
persistent identity: range1198221
display id: range1198221
version: 1
top level: BBa_I11031
direction: direction/forward
start: 382
end: 387
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214030
display id: annotation2214030
version: 1
top level: BBa_I11031
location: https://synbiohub.org/public/igem/BBa_I11031/annotation2214030/range2214030/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_I11031/annotation2214030/range2214030/1
type: Range
persistent identity: range2214030
display id: range2214030
version: 1
top level: BBa_I11031
direction: direction/forward
start: 388
end: 412
orientation: inline

S*Tag
type: ComponentDefinition
persistent identity: BBa J32017
display id: BBa_J32017
version: 1
was generated by: iGEM to SBOL conversion
description: S-Tag
created: 2006-08-24T11:00:00Z
modified: 2015-08-31T04:08:46Z
mutable provenance: Novagen
owned by: james
owned by: myers
mutable description: The S???Tag sequence is a novel fusion peptide tag for recombinant proteins that allows detection by a rapid, sensitive homogeneous assay or by colorimetric detection in Western blots. Proteins can also be rapidly purified from crude extracts using a one step affinity separation method. The system is based on the strong interaction between the 15aa S???Tag and 103aa S-protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: .
star: false
creator: Austen Heinz
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: S-Tag
sequence: https://synbiohub.org/public/igem/BBa_J32017_sequence/1

S-Tag
type: SequenceAnnotation
persistent identity: annotation1898184
display id: annotation1898184
version: 1
top level: S*Tag
location: https://synbiohub.org/public/igem/BBa_J32017/annotation1898184/range1898184/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J32017/annotation1898184/range1898184/1
type: Range
persistent identity: range1898184
display id: range1898184
version: 1
top level: S*Tag
direction: direction/forward
start: 1
end: 46
orientation: inline

BBa_J23012
type: ComponentDefinition
persistent identity: BBa J23012
display id: BBa_J23012
version: 1
was generated by: iGEM to SBOL conversion
description: Spectinomycin R open-reading-frame basic part
created: 2006-07-23T11:00:00Z
modified: 2015-08-31T04:08:38Z
mutable provenance: PCR JL1/JL2 on wt. pSPlam (917bp, EcoRI/SpeI, Neb2) Paste into pSB1A2-I13521 (EcoRI/SpeI, Neb2, Large 2062)
owned by: james
owned by: myers
mutable description: it is a Spec resistant biobrick
discontinued: false
dominant: false
experience: experience/Issues
group u list: _52_
m user id: 0
owner id: 363
owning group id: 52
sample status: In stock
status: status/Available
bookmark: false
mutable notes: JL1 Forward EcoRI oligo to bioBrick SpecR: gacttgaattcgcggccgcttctagagtccaagcgagctcgatatc JL2 Reverse SpeI oligo to bioBrick SpecR: ccgctactagtCAGCAACGATGTTACGCAGC
star: false
creator: Will Bosworth
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence annotation: SpR Spectinomycin Resistance
sequence: https://synbiohub.org/public/igem/BBa_J23012_sequence/1

SpR Spectinomycin Resistance
type: SequenceAnnotation
persistent identity: annotation1966465
display id: annotation1966465
version: 1
top level: BBa_J23012
location: https://synbiohub.org/public/igem/BBa_J23012/annotation1966465/range1966465/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J23012/annotation1966465/range1966465/1
type: Range
persistent identity: range1966465
display id: range1966465
version: 1
top level: BBa_J23012
direction: direction/reverse
start: 46
end: 837
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

PelB
type: ComponentDefinition
persistent identity: BBa J32015
display id: BBa_J32015
version: 1
was generated by: iGEM to SBOL conversion
description: PelB leader sequence; directs protein to E. coli periplasmic membrane
created: 2006-08-24T11:00:00Z
modified: 2015-08-31T04:08:46Z
mutable provenance: Novagen
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The pelB leader sequence is a sequence of amino acids which when attached to a protein, directs the protein to the periplasmic membrane of E. coli, where the sequence is removed by pelB peptidase. It is used to direct coat protein-antigen fusions to the cell surface.
discontinued: false
dominant: true
experience: experience/None
group u list: _50_
m user id: 0
owner id: 495
owning group id: 50
sample status: In stock
status: status/Available
bookmark: true
mutable notes: .
star: true
creator: Austen Heinz
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence annotation: PelB
sequence: https://synbiohub.org/public/igem/BBa_J32015_sequence/1

PelB
type: SequenceAnnotation
persistent identity: annotation1898182
display id: annotation1898182
version: 1
top level: PelB
location: https://synbiohub.org/public/igem/BBa_J32015/annotation1898182/range1898182/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_J32015/annotation1898182/range1898182/1
type: Range
persistent identity: range1898182
display id: range1898182
version: 1
top level: PelB
direction: direction/forward
start: 1
end: 66
orientation: inline

https://synbiohub.org/public/igem/BBa_I754002_sequence/1
type: Sequence
persistent identity: BBa I754002 sequence
display id: BBa_I754002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcctactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I11031_sequence/1
type: Sequence
persistent identity: BBa I11031 sequence
display id: BBa_I11031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaatcacacagcctcacacttgatgaggcctgtgcatttctcaagatatccagacctaccgccaccaactggattcgcacaggccgactacaggcaacacgtaaagaccccaccaaaccgaaatccccttacctcaccacacgacaagcctgcattgcggcacttcaatctccgctgcatactgtccaggtgagcgcgggtgatgacataacagaggaactgaaatgtcactattccgcagaggtgaaacctggtacgccagtttcacattgccgaacggcaaaagatttaagcagtctcttgggacaaaggacaaaaggcaggccacagagcttcatgacaagcgcagcaaacgacgaaaactacgctgctgctgtttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J32017_sequence/1
type: Sequence
persistent identity: BBa J32017 sequence
display id: BBa_J32017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaagaaaccgctgctgctaaattcgaacgccagcacatggacagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23012_sequence/1
type: Sequence
persistent identity: BBa J23012 sequence
display id: BBa_J23012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccaagcgagctcgatatccgtcggcttgaacgaattgttagacattatttgccgactaccttggtgatctcgcctttcacgtagtggacaaattcttccaactgatctgcgcgcgaggccaagcgatcttcttcttgtccaagataagcctgtctagcttcaagtatgacgggctgatactgggccggcaggcgctccattgcccagtcggcagcgacatccttcggcgcgattttgccggttactgcgctgtaccaaatgcgggacaacgtaagcactacatttcgctcatcgccagcccagtcgggcggcgagttccatagcgttaaggtttcatttagcgcctcaaatagatcctgttcaggaaccggatcaaagagttcctccgccgctggacctaccaaggcaacgctatgttctcttgcttttgtcagcaagatagccagatcaatgtcgtacgtggctggctcgaagatacctgcaagaatgtcattgcgctgccattctccaaattgcagttcgcgcttagctggataacgccacggaatgatgtcgtcgtgcacaacaatggtgacttctacagcgcggagaatctcgctctctccaggggaagccgaagtttccaaaaggtcgttgatcaaagctcgccgcgttgtttcatcaagccttacggtcaccgtaaccagcaaatcaatatcactgtgtggcttcaggccgccatccactgcggagccgtacaaatgtacggccagcaacgtcggttcgagatggcgctcgatgacgccaactacctctgatagttgagtcgatacttcggcgatcaccgcttccctcatgatgtttaactttgttttagggcgactgccctgctgcgtaacatcgttgct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J32015_sequence/1
type: Sequence
persistent identity: BBa J32015 sequence
display id: BBa_J32015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z